################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspI AvaI AviII AvrII BanI BbeI BbrPI BcgI BclI BfrI BlnI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BspLU11I BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccII 88 CGCG CG^CG AflIII 20 ACACGT A^C[GA][CT]GT AocI 775 CCTGAGG CC^T[ACGT]AGG Asp700I 1062 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 120 TGGCCA TGG^CCA BamHI 1206 GGATCC G^GATCC BbvCI 1053 GCTGAGG CCTCAGC(-5/-2) BciVI 24 GTATCC GTATCC(6/5) BcnI 600 CCGGG CC^[GC]GG BfaI 526 CTAG C^TAG BglI 43 GCCTTCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1301 AGATCT A^GATCT BsaAI 21 CACGTA [CT]AC^GT[GA] Bsp1407I 17 TGTACA T^GTACA BspHI 1007 TCATGA T^CATGA BsrBI 739 GAGCGG CCGCTC(-3/-3) BsrFI 163 GCCGGC [GA]^CCGG[CT] BsrGI 17 TGTACA T^GTACA BstUI 88 CGCG CG^CG BstXI 922 CCATATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 775 CCTGAGG CC^T[ACGT]AGG Cfr10I 163 GCCGGC [GA]^CCGG[CT] DsaI 691 CCACGG C^C[GA][CT]GG EaeI 120 TGGCCA [CT]^GGCC[GA] EarI 715 GAAGAG CTCTTC(1/4) Eco81I 775 CCTGAGG CC^T[ACGT]AGG EcoRI 410 GAATTC G^AATTC HaeII 804 GGCGCT [GA]GCGC^[CT] HgaI 1230 GACGC GACGC(5/10) Ksp632I 715 GAAGAG CTCTTC(1/4) MaeI 526 CTAG C^TAG MaeII 22 ACGT A^CGT MaeIII 469 GTGAC ^GT[ACGT]AC MluNI 120 TGGCCA TGG^CCA MscI 120 TGGCCA TGG^CCA MvnI 88 CGCG CG^CG NaeI 163 GCCGGC GCC^GGC NciI 600 CCGGG CC^[GC]GG NgoMIV 163 GCCGGC G^CCGGC NspI 1272 GCATGT [GA]CATG^[CT] RcaI 1007 TCATGA T^CATGA SspBI 17 TGTACA T^GTACA StuI 545 AGGCCT AGG^CCT TaqI 1239 TCGA T^CGA Tsp45I 469 GTGAC ^GT[GC]AC TthHB8I 1239 TCGA T^CGA XbaI 525 TCTAGA T^CTAGA XmnI 1062 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AhdI 949 GACGGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1177 GACTACGAGTC AlwI 1207 GATCC GGATC(4/5) 1206 GGATC AspEI 949 GACGGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1177 GACTACGAGTC AspHI 91 GTGCTC G[AT]GC[AT]^C 934 GAGCAC BanII 77 GGGCTC G[GA]GC[CT]^C 817 GAGCCC BbsI 438 GAAGAC GAAGAC(2/6) 1126 GTCTTC BlpI 736 GCTGAGC GC^T[ACGT]AGC 814 GCTGAGC Bpu1102I 736 GCTGAGC GC^T[ACGT]AGC 814 GCTGAGC BpuAI 438 GAAGAC GAAGAC(2/6) 1126 GTCTTC BsaI 667 GAGACC GGTCTC(1/5) 1222 GAGACC BsgI 495 CTGCAC GTGCAG(16/14) 286 GTGCAG BsiHKAI 91 GTGCTC G[AT]GC[AT]^C 934 GAGCAC BsmAI 667 GAGAC GTCTC(1/5) 1222 GAGAC BsmI 387 GAATGC GAATGC(1/-1) 1062 GAATGC BsrDI 222 CATTGC GCAATG(2/0) 239 GCAATG BstYI 1206 GGATCC [GA]^GATC[CT] 1301 AGATCT CelII 736 GCTGAGC GC^T[ACGT]AGC 814 GCTGAGC CfoI 805 GCGC GCG^C 882 GCGC DpnII 1207 GATC ^GATC 1302 GATC Eam1105I 949 GACGGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1177 GACTACGAGTC FauI 74 GCGGG CCCGC(4/6) 252 GCGGG HapII 164 CCGG C^CGG 600 CCGG HhaI 805 GCGC GCG^C 882 GCGC HinP1I 805 GCGC G^CGC 882 GCGC HpaII 164 CCGG C^CGG 600 CCGG MboI 1207 GATC ^GATC 1302 GATC MflI 1206 GGATCC [GA]^GATC[CT] 1301 AGATCT MseI 584 TTAA T^TAA 878 TTAA MslI 1026 CACTGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1187 CATACACATG MspI 164 CCGG C^CGG 600 CCGG NdeII 1207 GATC ^GATC 1302 GATC Sau3AI 1207 GATC ^GATC 1302 GATC TfiI 197 GATTC G^A[AT]TC 307 GATTC Tru9I 584 TTAA T^TAA 878 TTAA XcmI 108 CCACTTCCATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 325 CCAGCCAGTACCTGG XhoII 1206 GGATCC [GA]^GATC[CT] 1301 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AciI AcsI AfaI AluI AlwNI ApoI AvaII BbvI BmyI BpmI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DraII EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MboII MnlI MspA1I MvaI MwoI NlaIII NlaIV PflMI PleI PpuMI RsaI Sau96I ScaI ScrFI SfaNI StyI TseI Tsp509I TspRI Van91I