##################################
# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspI AvaI AviII AvrII
BanI BbeI BbrPI BcgI BclI
BfrI BlnI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsoBI
BspDI BspEI BspLU11I BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI DraIII
DrdI EagI EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRV EcoT22I FbaI
FseI FspI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
NarI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccII 88 CGCG CG^CG
AflIII 20 ACACGT A^C[GA][CT]GT
AocI 775 CCTGAGG CC^T[ACGT]AGG
Asp700I 1062 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 120 TGGCCA TGG^CCA
BamHI 1206 GGATCC G^GATCC
BbvCI 1053 GCTGAGG CCTCAGC(-5/-2)
BciVI 24 GTATCC GTATCC(6/5)
BcnI 600 CCGGG CC^[GC]GG
BfaI 526 CTAG C^TAG
BglI 43 GCCTTCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1301 AGATCT A^GATCT
BsaAI 21 CACGTA [CT]AC^GT[GA]
Bsp1407I 17 TGTACA T^GTACA
BspHI 1007 TCATGA T^CATGA
BsrBI 739 GAGCGG CCGCTC(-3/-3)
BsrFI 163 GCCGGC [GA]^CCGG[CT]
BsrGI 17 TGTACA T^GTACA
BstUI 88 CGCG CG^CG
BstXI 922 CCATATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 775 CCTGAGG CC^T[ACGT]AGG
Cfr10I 163 GCCGGC [GA]^CCGG[CT]
DsaI 691 CCACGG C^C[GA][CT]GG
EaeI 120 TGGCCA [CT]^GGCC[GA]
EarI 715 GAAGAG CTCTTC(1/4)
Eco81I 775 CCTGAGG CC^T[ACGT]AGG
EcoRI 410 GAATTC G^AATTC
HaeII 804 GGCGCT [GA]GCGC^[CT]
HgaI 1230 GACGC GACGC(5/10)
Ksp632I 715 GAAGAG CTCTTC(1/4)
MaeI 526 CTAG C^TAG
MaeII 22 ACGT A^CGT
MaeIII 469 GTGAC ^GT[ACGT]AC
MluNI 120 TGGCCA TGG^CCA
MscI 120 TGGCCA TGG^CCA
MvnI 88 CGCG CG^CG
NaeI 163 GCCGGC GCC^GGC
NciI 600 CCGGG CC^[GC]GG
NgoMIV 163 GCCGGC G^CCGGC
NspI 1272 GCATGT [GA]CATG^[CT]
RcaI 1007 TCATGA T^CATGA
SspBI 17 TGTACA T^GTACA
StuI 545 AGGCCT AGG^CCT
TaqI 1239 TCGA T^CGA
Tsp45I 469 GTGAC ^GT[GC]AC
TthHB8I 1239 TCGA T^CGA
XbaI 525 TCTAGA T^CTAGA
XmnI 1062 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AhdI 949 GACGGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1177 GACTACGAGTC
AlwI 1207 GATCC GGATC(4/5)
1206 GGATC
AspEI 949 GACGGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1177 GACTACGAGTC
AspHI 91 GTGCTC G[AT]GC[AT]^C
934 GAGCAC
BanII 77 GGGCTC G[GA]GC[CT]^C
817 GAGCCC
BbsI 438 GAAGAC GAAGAC(2/6)
1126 GTCTTC
BlpI 736 GCTGAGC GC^T[ACGT]AGC
814 GCTGAGC
Bpu1102I 736 GCTGAGC GC^T[ACGT]AGC
814 GCTGAGC
BpuAI 438 GAAGAC GAAGAC(2/6)
1126 GTCTTC
BsaI 667 GAGACC GGTCTC(1/5)
1222 GAGACC
BsgI 495 CTGCAC GTGCAG(16/14)
286 GTGCAG
BsiHKAI 91 GTGCTC G[AT]GC[AT]^C
934 GAGCAC
BsmAI 667 GAGAC GTCTC(1/5)
1222 GAGAC
BsmI 387 GAATGC GAATGC(1/-1)
1062 GAATGC
BsrDI 222 CATTGC GCAATG(2/0)
239 GCAATG
BstYI 1206 GGATCC [GA]^GATC[CT]
1301 AGATCT
CelII 736 GCTGAGC GC^T[ACGT]AGC
814 GCTGAGC
CfoI 805 GCGC GCG^C
882 GCGC
DpnII 1207 GATC ^GATC
1302 GATC
Eam1105I 949 GACGGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1177 GACTACGAGTC
FauI 74 GCGGG CCCGC(4/6)
252 GCGGG
HapII 164 CCGG C^CGG
600 CCGG
HhaI 805 GCGC GCG^C
882 GCGC
HinP1I 805 GCGC G^CGC
882 GCGC
HpaII 164 CCGG C^CGG
600 CCGG
MboI 1207 GATC ^GATC
1302 GATC
MflI 1206 GGATCC [GA]^GATC[CT]
1301 AGATCT
MseI 584 TTAA T^TAA
878 TTAA
MslI 1026 CACTGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1187 CATACACATG
MspI 164 CCGG C^CGG
600 CCGG
NdeII 1207 GATC ^GATC
1302 GATC
Sau3AI 1207 GATC ^GATC
1302 GATC
TfiI 197 GATTC G^A[AT]TC
307 GATTC
Tru9I 584 TTAA T^TAA
878 TTAA
XcmI 108 CCACTTCCATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
325 CCAGCCAGTACCTGG
XhoII 1206 GGATCC [GA]^GATC[CT]
1301 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI AvaII BbvI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DraII
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI MboII
MnlI MspA1I MvaI MwoI NlaIII
NlaIV PflMI PleI PpuMI RsaI
Sau96I ScaI ScrFI SfaNI StyI
TseI Tsp509I TspRI Van91I