Restriction map for pF1KB0972
##################################
# NO CUT ENZYMES (  120 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AgeI        Alw44I      Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspI        AvaI        AviII       AvrII       
BanI        BbeI        BbrPI       BcgI        BclI        
BfrI        BlnI        BsaBI       BsaHI       BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsoBI       
BspDI       BspEI       BspLU11I    BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     ClaI        CpoI        DraI        DraIII      
DrdI        EagI        EclXI       Eco47III    Eco52I      
EcoNI       EcoO65I     EcoRV       EcoT22I     FbaI        
FseI        FspI        Hin1I       HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MroI        MunI        
NarI        NcoI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RsrII       SacI        SacII       SalI        
SapI        SbfI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
SwaI        Tth111I     XhoI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccII          88  CGCG                 CG^CG
AflIII         20  ACACGT               A^C[GA][CT]GT
AocI          775  CCTGAGG              CC^T[ACGT]AGG
Asp700I      1062  GAATGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI          120  TGGCCA               TGG^CCA
BamHI        1206  GGATCC               G^GATCC
BbvCI        1053  GCTGAGG              CCTCAGC(-5/-2)
BciVI          24  GTATCC               GTATCC(6/5)
BcnI          600  CCGGG                CC^[GC]GG
BfaI          526  CTAG                 C^TAG
BglI           43  GCCTTCAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1301  AGATCT               A^GATCT
BsaAI          21  CACGTA               [CT]AC^GT[GA]
Bsp1407I       17  TGTACA               T^GTACA
BspHI        1007  TCATGA               T^CATGA
BsrBI         739  GAGCGG               CCGCTC(-3/-3)
BsrFI         163  GCCGGC               [GA]^CCGG[CT]
BsrGI          17  TGTACA               T^GTACA
BstUI          88  CGCG                 CG^CG
BstXI         922  CCATATTTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        775  CCTGAGG              CC^T[ACGT]AGG
Cfr10I        163  GCCGGC               [GA]^CCGG[CT]
DsaI          691  CCACGG               C^C[GA][CT]GG
EaeI          120  TGGCCA               [CT]^GGCC[GA]
EarI          715  GAAGAG               CTCTTC(1/4)
Eco81I        775  CCTGAGG              CC^T[ACGT]AGG
EcoRI         410  GAATTC               G^AATTC
HaeII         804  GGCGCT               [GA]GCGC^[CT]
HgaI         1230  GACGC                GACGC(5/10)
Ksp632I       715  GAAGAG               CTCTTC(1/4)
MaeI          526  CTAG                 C^TAG
MaeII          22  ACGT                 A^CGT
MaeIII        469  GTGAC                ^GT[ACGT]AC
MluNI         120  TGGCCA               TGG^CCA
MscI          120  TGGCCA               TGG^CCA
MvnI           88  CGCG                 CG^CG
NaeI          163  GCCGGC               GCC^GGC
NciI          600  CCGGG                CC^[GC]GG
NgoMIV        163  GCCGGC               G^CCGGC
NspI         1272  GCATGT               [GA]CATG^[CT]
RcaI         1007  TCATGA               T^CATGA
SspBI          17  TGTACA               T^GTACA
StuI          545  AGGCCT               AGG^CCT
TaqI         1239  TCGA                 T^CGA
Tsp45I        469  GTGAC                ^GT[GC]AC
TthHB8I      1239  TCGA                 T^CGA
XbaI          525  TCTAGA               T^CTAGA
XmnI         1062  GAATGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

AhdI          949  GACGGCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1177  GACTACGAGTC         
AlwI         1207  GATCC                GGATC(4/5)
             1206  GGATC               
AspEI         949  GACGGCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1177  GACTACGAGTC         
AspHI          91  GTGCTC               G[AT]GC[AT]^C
              934  GAGCAC              
BanII          77  GGGCTC               G[GA]GC[CT]^C
              817  GAGCCC              
BbsI          438  GAAGAC               GAAGAC(2/6)
             1126  GTCTTC              
BlpI          736  GCTGAGC              GC^T[ACGT]AGC
              814  GCTGAGC             
Bpu1102I      736  GCTGAGC              GC^T[ACGT]AGC
              814  GCTGAGC             
BpuAI         438  GAAGAC               GAAGAC(2/6)
             1126  GTCTTC              
BsaI          667  GAGACC               GGTCTC(1/5)
             1222  GAGACC              
BsgI          495  CTGCAC               GTGCAG(16/14)
              286  GTGCAG              
BsiHKAI        91  GTGCTC               G[AT]GC[AT]^C
              934  GAGCAC              
BsmAI         667  GAGAC                GTCTC(1/5)
             1222  GAGAC               
BsmI          387  GAATGC               GAATGC(1/-1)
             1062  GAATGC              
BsrDI         222  CATTGC               GCAATG(2/0)
              239  GCAATG              
BstYI        1206  GGATCC               [GA]^GATC[CT]
             1301  AGATCT              
CelII         736  GCTGAGC              GC^T[ACGT]AGC
              814  GCTGAGC             
CfoI          805  GCGC                 GCG^C
              882  GCGC                
DpnII        1207  GATC                 ^GATC
             1302  GATC                
Eam1105I      949  GACGGCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1177  GACTACGAGTC         
FauI           74  GCGGG                CCCGC(4/6)
              252  GCGGG               
HapII         164  CCGG                 C^CGG
              600  CCGG                
HhaI          805  GCGC                 GCG^C
              882  GCGC                
HinP1I        805  GCGC                 G^CGC
              882  GCGC                
HpaII         164  CCGG                 C^CGG
              600  CCGG                
MboI         1207  GATC                 ^GATC
             1302  GATC                
MflI         1206  GGATCC               [GA]^GATC[CT]
             1301  AGATCT              
MseI          584  TTAA                 T^TAA
              878  TTAA                
MslI         1026  CACTGGAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1187  CATACACATG          
MspI          164  CCGG                 C^CGG
              600  CCGG                
NdeII        1207  GATC                 ^GATC
             1302  GATC                
Sau3AI       1207  GATC                 ^GATC
             1302  GATC                
TfiI          197  GATTC                G^A[AT]TC
              307  GATTC               
Tru9I         584  TTAA                 T^TAA
              878  TTAA                
XcmI          108  CCACTTCCATTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              325  CCAGCCAGTACCTGG     
XhoII        1206  GGATCC               [GA]^GATC[CT]
             1301  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   54 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwNI       
ApoI        AvaII       BbvI        BmyI        BpmI        
BsaJI       BseRI       BsiYI       BslI        BsmFI       
Bsp1286I    BspMI       BsrI        BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        DraII       
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HinfI       HphI        ItaI        MboII       
MnlI        MspA1I      MvaI        MwoI        NlaIII      
NlaIV       PflMI       PleI        PpuMI       RsaI        
Sau96I      ScaI        ScrFI       SfaNI       StyI        
TseI        Tsp509I     TspRI       Van91I      
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