Restriction map for
pF1KB0975
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# NO CUT ENZYMES ( 152 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AfaI AflII AflIII AgeI AhdI
AlwI AlwNI AocI Aor51HI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BfrI BglI BglII BlpI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstXI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DraI DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FokI FseI FspI HaeII
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MflI
MluI MluNI MroI MscI MunI
NarI NcoI NdeI NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp45I Tsp509I Tth111I XcmI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AatII 201 GACGTC GACGT^C
AcyI 201 GACGTC G[GA]^CG[CT]C
ApaI 528 GGGCCC GGGCC^C
AvrII 196 CCTAGG C^CTAGG
BanI 84 GGTGCC G^G[CT][GA]CC
BanII 528 GGGCCC G[GA]GC[CT]^C
BbsI 572 GTCTTC GAAGAC(2/6)
BclI 260 TGATCA T^GATCA
BlnI 196 CCTAGG C^CTAGG
BpmI 412 CTGGAG CTGGAG(16/14)
BpuAI 572 GTCTTC GAAGAC(2/6)
BsaHI 201 GACGTC G[GA]^CG[CT]C
BsrFI 90 GCCGGC [GA]^CCGG[CT]
BstNI 498 CCAGG CC^[AT]GG
Cfr10I 90 GCCGGC [GA]^CCGG[CT]
DpnII 261 GATC ^GATC
DraII 116 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 431 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 625 GAAGAG CTCTTC(1/4)
EcoO109I 116 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRII 498 CCAGG ^CC[AT]GG
FbaI 260 TGATCA T^GATCA
Hin1I 201 GACGTC G[GA]^CG[CT]C
Ksp632I 625 GAAGAG CTCTTC(1/4)
MaeIII 28 GTTAC ^GT[ACGT]AC
MboI 261 GATC ^GATC
MslI 594 CATTAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 287 CCGCTG C[AC]G^C[GT]G
MvaI 498 CCAGG CC^[AT]GG
NaeI 90 GCCGGC GCC^GGC
NdeII 261 GATC ^GATC
NgoMIV 90 GCCGGC G^CCGGC
PflMI 40 CCAGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 53 GAGTC GAGTC(4/5)
PpuMI 116 GGGACCC [GA]G^G[AT]CC[CT]
SapI 625 GAAGAGC GCTCTTC(1/4)
Sau3AI 261 GATC ^GATC
SfcI 312 CTGTAG C^T[GA][CT]AG
SgrAI 89 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 508 CCCGGG CCC^GGG
TaqI 419 TCGA T^CGA
TfiI 272 GAATC G^A[AT]TC
TthHB8I 419 TCGA T^CGA
Van91I 40 CCAGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 339 TCTAGA T^CTAGA
XmaCI 508 CCCGGG C^CCGGG
XmaI 508 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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Alw44I 212 GTGCAC G^TGCAC
292 GTGCAC
ApaLI 212 GTGCAC G^TGCAC
292 GTGCAC
AvaI 229 CTCGGG C^[CT]CG[GA]G
508 CCCGGG
AvaII 117 GGACC G^G[AT]CC
178 GGACC
BseRI 147 CTCCTC GAGGAG(10/8)
519 GAGGAG
BsgI 77 GTGCAG GTGCAG(16/14)
379 GTGCAG
BsmAI 442 GAGAC GTCTC(1/5)
204 GTCTC
BsmBI 203 CGTCTC CGTCTC(1/5)
442 GAGACG
BsoBI 229 CTCGGG C^[CT]CG[GA]G
508 CCCGGG
BspMI 73 GCAGGT ACCTGC(4/8)
513 GCAGGT
DdeI 268 CTCAG C^T[ACGT]AG
440 CTGAG
EcoNI 191 CCTCACCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
337 CCTCTAGAAGG
EcoT14I 45 CCTTGG C^C[AT][AT]GG
196 CCTAGG
HgaI 143 GACGC GACGC(5/10)
444 GACGC
HinfI 53 GAGTC G^A[ACGT]TC
272 GAATC
MaeII 31 ACGT A^CGT
202 ACGT
MboII 625 GAAGA GAAGA(8/7)
573 TCTTC
MseI 361 TTAA T^TAA
596 TTAA
StyI 45 CCTTGG C^C[AT][AT]GG
196 CCTAGG
Tru9I 361 TTAA T^TAA
596 TTAA
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AccII AciI AluI AspHI BbvI
BcnI BfaI BmyI BsaJI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BssKI
BstUI Cac8I CfoI Cfr13I FauI
Fnu4HI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MaeI MnlI
MspI MvnI MwoI NciI NlaIV
Sau96I ScrFI TseI TspRI