################################## # NO CUT ENZYMES ( 194 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsiEI BsiWI BsiYI BslI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DdeI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoT14I EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI ItaI KasI KpnI KspI MaeI MaeII MamI MfeI MflI MluI MluNI MroI MscI MseI MslI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacII SalI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tru9I TseI TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AciI 158 CCGC CCGC(-3/-1) AcsI 184 GAATTC [GA]^AATT[CT] AfaI 18 GTAC GT^AC AlwI 174 GATCC GGATC(4/5) ApoI 184 GAATTC [GA]^AATT[CT] Asp700I 135 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 79 GAGCTC G[AT]GC[AT]^C AspI 349 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 164 GGTCC G^G[AT]CC BanII 79 GAGCTC G[GA]GC[CT]^C BbsI 355 GTCTTC GAAGAC(2/6) BmyI 79 GAGCTC G[AGT]GC[ACT]^C BpmI 211 CTGGAG CTGGAG(16/14) BpuAI 355 GTCTTC GAAGAC(2/6) BsgI 124 GTGCAG GTGCAG(16/14) BsiHKAI 79 GAGCTC G[AT]GC[AT]^C BsmAI 214 GAGAC GTCTC(1/5) Bsp1286I 79 GAGCTC G[AGT]GC[ACT]^C BspHI 53 TCATGA T^CATGA BsrI 270 ACTGG ACTGG(1/-1) BssKI 33 CCAGG ^CC[ACGT]GG BstF5I 84 CATCC GGATG(2/0) BstNI 33 CCAGG CC^[AT]GG BstXI 335 CCATGACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr13I 164 GGTCC G^G[ACGT]CC DpnII 174 GATC ^GATC EarI 76 GAAGAG CTCTTC(1/4) EcoRI 184 GAATTC G^AATTC EcoRII 33 CCAGG ^CC[AT]GG EcoRV 100 GATATC GAT^ATC FokI 84 CATCC GGATG(9/13) HinfI 365 GAATC G^A[ACGT]TC Ksp632I 76 GAAGAG CTCTTC(1/4) MboI 174 GATC ^GATC MnlI 199 GAGG CCTC(7/6) MspA1I 158 CCGCTG C[AC]G^C[GT]G MvaI 33 CCAGG CC^[AT]GG MwoI 72 GCCGGAAGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeII 174 GATC ^GATC NlaIV 163 GGGTCC GG[ACGT]^[ACGT]CC PflFI 349 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 25 CTGCAG CTGCA^G RcaI 53 TCATGA T^CATGA RsaI 18 GTAC GT^AC SacI 79 GAGCTC GAGCT^C SapI 76 GAAGAGC GCTCTTC(1/4) Sau3AI 174 GATC ^GATC Sau96I 164 GGTCC G^G[ACGT]CC ScrFI 33 CCAGG CC^[ACGT]GG SfcI 25 CTGCAG C^T[GA][CT]AG TfiI 365 GAATC G^A[AT]TC TspRI 242 CAGTG CA[GC]TG[ACGT][ACGT]^ Tth111I 349 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 135 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 7 enzymes) # ################################### AluI 80 AGCT AG^CT 209 AGCT HapII 11 CCGG C^CGG 73 CCGG HpaII 11 CCGG C^CGG 73 CCGG MboII 76 GAAGA GAAGA(8/7) 356 TCTTC MspI 11 CCGG C^CGG 73 CCGG NlaIII 54 CATG CATG^ 336 CATG Tsp509I 185 AATT ^AATT 378 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 3 enzymes) # ######################################### HphI MaeIII Tsp45I