Restriction map for
pF1KB6852
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# NO CUT ENZYMES ( 180 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiWI BsmAI
BsmBI BsmFI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII CfoI
ClaI CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI KasI KpnI KspI
MaeIII MamI MfeI MflI MluI
MluNI MroI MscI MspA1I MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacII SalI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tsp45I TspRI Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AcsI 160 GAATTC [GA]^AATT[CT]
AflII 213 CTTAAG C^TTAAG
ApoI 160 GAATTC [GA]^AATT[CT]
Asp700I 331 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 239 GAGCTC G[AT]GC[AT]^C
AvaII 372 GGACC G^G[AT]CC
BanII 239 GAGCTC G[GA]GC[CT]^C
BcnI 57 CCCGG CC^[GC]GG
BfaI 466 CTAG C^TAG
BfrI 213 CTTAAG C^TTAAG
BglI 508 GCCCTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 71 CTGCAC GTGCAG(16/14)
BsiHKAI 239 GAGCTC G[AT]GC[AT]^C
BsmI 309 GAATGC GAATGC(1/-1)
BsrFI 63 ACCGGC [GA]^CCGG[CT]
BstF5I 156 GGATG GGATG(2/0)
Cac8I 351 GCAAGC GC[ACGT]^[ACGT]GC
Cfr10I 63 ACCGGC [GA]^CCGG[CT]
Cfr13I 372 GGACC G^G[ACGT]CC
DdeI 143 CTAAG C^T[ACGT]AG
EarI 301 GAAGAG CTCTTC(1/4)
EcoRI 160 GAATTC G^AATTC
FokI 156 GGATG GGATG(9/13)
HaeIII 378 GGCC GG^CC
HindIII 554 AAGCTT A^AGCTT
Ksp632I 301 GAAGAG CTCTTC(1/4)
MaeI 466 CTAG C^TAG
MaeII 17 ACGT A^CGT
MslI 435 CACAGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 57 CCCGG CC^[GC]GG
PleI 529 GACTC GAGTC(4/5)
SacI 239 GAGCTC GAGCT^C
SapI 301 GAAGAGC GCTCTTC(1/4)
Sau96I 372 GGACC G^G[ACGT]CC
SmlI 213 CTTAAG C^T[CT][GA]AG
TfiI 199 GATTC G^A[AT]TC
XmnI 331 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
BbsI 388 GAAGAC GAAGAC(2/6)
526 GAAGAC
BmyI 239 GAGCTC G[AGT]GC[ACT]^C
246 GGGCAC
BpuAI 388 GAAGAC GAAGAC(2/6)
526 GAAGAC
BsiYI 51 CCCAGCCCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
57 CCCGGCACCGG
BslI 51 CCCAGCCCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
57 CCCGGCACCGG
Bsp1286I 239 GAGCTC G[AGT]GC[ACT]^C
246 GGGCAC
BsrDI 296 GCAATG GCAATG(2/0)
428 GCAATG
DpnII 132 GATC ^GATC
207 GATC
HapII 58 CCGG C^CGG
64 CCGG
HinfI 199 GATTC G^A[ACGT]TC
529 GACTC
HpaII 58 CCGG C^CGG
64 CCGG
MboI 132 GATC ^GATC
207 GATC
MseI 128 TTAA T^TAA
214 TTAA
MspI 58 CCGG C^CGG
64 CCGG
NdeII 132 GATC ^GATC
207 GATC
Sau3AI 132 GATC ^GATC
207 GATC
SfaNI 348 GATGC GCATC(5/9)
542 GCATC
TaqI 134 TCGA T^CGA
237 TCGA
Tru9I 128 TTAA T^TAA
214 TTAA
TthHB8I 134 TCGA T^CGA
237 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AciI AluI BanI BbvI BsaJI
BspMI BssKI BstNI EcoRII Fnu4HI
HphI ItaI MboII MnlI MvaI
MwoI NlaIII NlaIV ScrFI TseI
Tsp509I