################################## # NO CUT ENZYMES ( 183 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AvaII AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglII BlnI BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsiYI BslI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoT14I EcoT22I FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MamI MfeI MflI MluI MroI MslI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AciI 483 GCGG CCGC(-3/-1) AflIII 66 ACATGT A^C[GA][CT]GT Asp700I 106 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 162 TGGCCA TGG^CCA BanII 360 GAGCTC G[GA]GC[CT]^C BfaI 416 CTAG C^TAG BglI 678 GCCCCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 127 GCTGAGC GC^T[ACGT]AGC BpmI 587 CTGGAG CTGGAG(16/14) Bpu1102I 127 GCTGAGC GC^T[ACGT]AGC BsaJI 196 CCTGGG C^C[ACGT][ACGT]GG BsmFI 3 GGGAC GGGAC(10/14) BspHI 290 TCATGA T^CATGA BspLU11I 66 ACATGT A^CATGT CelII 127 GCTGAGC GC^T[ACGT]AGC DdeI 128 CTGAG C^T[ACGT]AG EaeI 162 TGGCCA [CT]^GGCC[GA] EcoRV 325 GATATC GAT^ATC FauI 483 GCGGG CCCGC(4/6) HphI 435 GGTGA GGTGA(8/7) MaeI 416 CTAG C^TAG MaeII 73 ACGT A^CGT MluNI 162 TGGCCA TGG^CCA MscI 162 TGGCCA TGG^CCA MspA1I 442 CAGCTG C[AC]G^C[GT]G NlaIV 91 GGCTCC GG[ACGT]^[ACGT]CC NspI 66 ACATGT [GA]CATG^[CT] PvuII 442 CAGCTG CAG^CTG RcaI 290 TCATGA T^CATGA SacI 360 GAGCTC GAGCT^C SfcI 577 CTACAG C^T[GA][CT]AG XmnI 106 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AlwI 18 GGATC GGATC(4/5) 287 GGATC AspHI 360 GAGCTC G[AT]GC[AT]^C 506 GTGCTC BbvI 340 GCAGC GCAGC(8/12) 441 GCAGC BmyI 360 GAGCTC G[AGT]GC[ACT]^C 506 GTGCTC BseRI 454 GAGGAG GAGGAG(10/8) 457 GAGGAG BsiHKAI 360 GAGCTC G[AT]GC[AT]^C 506 GTGCTC Bsp1286I 360 GAGCTC G[AGT]GC[ACT]^C 506 GTGCTC BsrI 586 ACTGG ACTGG(1/-1) 303 CCAGT BssKI 165 CCAGG ^CC[ACGT]GG 196 CCTGG BstNI 165 CCAGG CC^[AT]GG 196 CCTGG EcoRII 165 CCAGG ^CC[AT]GG 196 CCTGG Fnu4HI 340 GCAGC GC^[ACGT]GC 441 GCAGC HinfI 205 GATTC G^A[ACGT]TC 598 GAATC ItaI 340 GCAGC GC^[ACGT]GC 441 GCAGC MvaI 165 CCAGG CC^[AT]GG 196 CCTGG ScrFI 165 CCAGG CC^[ACGT]GG 196 CCTGG SfaNI 616 GATGC GCATC(5/9) 280 GCATC TaqI 81 TCGA T^CGA 549 TCGA TfiI 205 GATTC G^A[AT]TC 598 GAATC TseI 340 GCAGC G^C[AT]GC 441 GCAGC TspRI 264 CAGTG CA[GC]TG[ACGT][ACGT]^ 624 CACTG TthHB8I 81 TCGA T^CGA 549 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AcsI AfaI AluI ApoI BstF5I Cac8I DpnII FokI HaeIII MaeIII MboI MboII MnlI MseI MwoI NdeII NlaIII RsaI Sau3AI Tru9I Tsp509I