Restriction map for pF1KB0979
##################################
# NO CUT ENZYMES (  183 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AvaI        AvaII       AviII       
AvrII       BamHI       BanI        BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BcnI        BfrI        BglII       BlnI        BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiWI       BsiYI       
BslI        BsmAI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
CfoI        Cfr10I      Cfr13I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        DsaI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoT14I     EcoT22I     FbaI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       KasI        KpnI        Ksp632I     
KspI        MamI        MfeI        MflI        MluI        
MroI        MslI        MspI        MunI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RsrII       SacII       SalI        SapI        
Sau96I      SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        Tsp45I      
Tth111I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AciI          483  GCGG                 CCGC(-3/-1)
AflIII         66  ACATGT               A^C[GA][CT]GT
Asp700I       106  GAAGAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI          162  TGGCCA               TGG^CCA
BanII         360  GAGCTC               G[GA]GC[CT]^C
BfaI          416  CTAG                 C^TAG
BglI          678  GCCCCACAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          127  GCTGAGC              GC^T[ACGT]AGC
BpmI          587  CTGGAG               CTGGAG(16/14)
Bpu1102I      127  GCTGAGC              GC^T[ACGT]AGC
BsaJI         196  CCTGGG               C^C[ACGT][ACGT]GG
BsmFI           3  GGGAC                GGGAC(10/14)
BspHI         290  TCATGA               T^CATGA
BspLU11I       66  ACATGT               A^CATGT
CelII         127  GCTGAGC              GC^T[ACGT]AGC
DdeI          128  CTGAG                C^T[ACGT]AG
EaeI          162  TGGCCA               [CT]^GGCC[GA]
EcoRV         325  GATATC               GAT^ATC
FauI          483  GCGGG                CCCGC(4/6)
HphI          435  GGTGA                GGTGA(8/7)
MaeI          416  CTAG                 C^TAG
MaeII          73  ACGT                 A^CGT
MluNI         162  TGGCCA               TGG^CCA
MscI          162  TGGCCA               TGG^CCA
MspA1I        442  CAGCTG               C[AC]G^C[GT]G
NlaIV          91  GGCTCC               GG[ACGT]^[ACGT]CC
NspI           66  ACATGT               [GA]CATG^[CT]
PvuII         442  CAGCTG               CAG^CTG
RcaI          290  TCATGA               T^CATGA
SacI          360  GAGCTC               GAGCT^C
SfcI          577  CTACAG               C^T[GA][CT]AG
XmnI          106  GAAGAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AlwI           18  GGATC                GGATC(4/5)
              287  GGATC               
AspHI         360  GAGCTC               G[AT]GC[AT]^C
              506  GTGCTC              
BbvI          340  GCAGC                GCAGC(8/12)
              441  GCAGC               
BmyI          360  GAGCTC               G[AGT]GC[ACT]^C
              506  GTGCTC              
BseRI         454  GAGGAG               GAGGAG(10/8)
              457  GAGGAG              
BsiHKAI       360  GAGCTC               G[AT]GC[AT]^C
              506  GTGCTC              
Bsp1286I      360  GAGCTC               G[AGT]GC[ACT]^C
              506  GTGCTC              
BsrI          586  ACTGG                ACTGG(1/-1)
              303  CCAGT               
BssKI         165  CCAGG                ^CC[ACGT]GG
              196  CCTGG               
BstNI         165  CCAGG                CC^[AT]GG
              196  CCTGG               
EcoRII        165  CCAGG                ^CC[AT]GG
              196  CCTGG               
Fnu4HI        340  GCAGC                GC^[ACGT]GC
              441  GCAGC               
HinfI         205  GATTC                G^A[ACGT]TC
              598  GAATC               
ItaI          340  GCAGC                GC^[ACGT]GC
              441  GCAGC               
MvaI          165  CCAGG                CC^[AT]GG
              196  CCTGG               
ScrFI         165  CCAGG                CC^[ACGT]GG
              196  CCTGG               
SfaNI         616  GATGC                GCATC(5/9)
              280  GCATC               
TaqI           81  TCGA                 T^CGA
              549  TCGA                
TfiI          205  GATTC                G^A[AT]TC
              598  GAATC               
TseI          340  GCAGC                G^C[AT]GC
              441  GCAGC               
TspRI         264  CAGTG                CA[GC]TG[ACGT][ACGT]^
              624  CACTG               
TthHB8I        81  TCGA                 T^CGA
              549  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   21 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        BstF5I      
Cac8I       DpnII       FokI        HaeIII      MaeIII      
MboI        MboII       MnlI        MseI        MwoI        
NdeII       NlaIII      RsaI        Sau3AI      Tru9I       
Tsp509I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com