Restriction map for
pF1KB0982
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# NO CUT ENZYMES ( 117 enzymes) #
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AatII Acc65I AclI AcsI AflII
AflIII AgeI AhdI AocI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AvrII BbsI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspLU11I BsrDI BsrFI BsrGI BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HindIII HpaI
KpnI KspI MaeI MaeIII MamI
MfeI MluI MseI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SbfI ScaI SexAI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tru9I Tsp45I Tsp509I Tth111I Van91I
XbaI XhoI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccIII 1301 TCCGGA T^CCGGA
Alw44I 301 GTGCAC G^TGCAC
AlwNI 139 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1091 AGCGCT AGC^GCT
ApaI 462 GGGCCC GGGCC^C
ApaLI 301 GTGCAC G^TGCAC
AviII 578 TGCGCA TGC^GCA
BamHI 1307 GGATCC G^GATCC
BbeI 479 GGCGCC GGCGC^C
BbrPI 246 CACGTG CAC^GTG
BsaI 778 GAGACC GGTCTC(1/5)
BsaWI 1301 TCCGGA [AT]^CCGG[AT]
BseAI 1301 TCCGGA T^CCGGA
BspEI 1301 TCCGGA T^CCGGA
BspHI 560 TCATGA T^CATGA
BspMI 575 ACCTGC ACCTGC(4/8)
BsrBI 120 CCGCTC CCGCTC(-3/-3)
Eco47III 1091 AGCGCT AGC^GCT
EcoNI 1298 CCTTCCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 578 TGCGCA TGC^GCA
HgaI 1062 GCGTC GACGC(5/10)
HinfI 649 GAGTC G^A[ACGT]TC
KasI 479 GGCGCC G^GCGCC
MroI 1301 TCCGGA T^CCGGA
NarI 479 GGCGCC GG^CGCC
PleI 649 GAGTC GAGTC(4/5)
PmaCI 246 CACGTG CAC^GTG
PmlI 246 CACGTG CAC^GTG
PstI 970 CTGCAG CTGCA^G
PvuII 1075 CAGCTG CAG^CTG
RcaI 560 TCATGA T^CATGA
SalI 1064 GTCGAC G^TCGAC
SapI 279 GCTCTTC GCTCTTC(1/4)
SfiI 30 GGCCACAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 479 GGCGCC GGC^GCC
StuI 506 AGGCCT AGG^CCT
XcmI 212 CCAACGAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
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AccI 571 GTCTAC GT^[AC][GT]AC
1064 GTCGAC
AcyI 479 GGCGCC G[GA]^CG[CT]C
1061 GGCGTC
Asp700I 141 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1089 GAAGCGCTTC
BanI 479 GGCGCC G^G[CT][GA]CC
1197 GGTGCC
BanII 462 GGGCCC G[GA]GC[CT]^C
984 GGGCTC
BbvCI 689 CCTCAGC CCTCAGC(-5/-2)
950 CCTCAGC
BsaAI 246 CACGTG [CT]AC^GT[GA]
1007 TACGTG
BsaHI 479 GGCGCC G[GA]^CG[CT]C
1061 GGCGTC
BsgI 285 CTGCAC GTGCAG(16/14)
25 GTGCAG
BsmAI 778 GAGAC GTCTC(1/5)
942 GTCTC
BsmFI 796 GGGAC GGGAC(10/14)
617 GTCCC
BssHII 129 GCGCGC G^CGCGC
347 GCGCGC
BstAPI 245 GCACGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
383 GCATCAAGTGC
BstXI 314 CCATCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
811 CCACCCAGTTGG
BstYI 535 GGATCT [GA]^GATC[CT]
1307 GGATCC
DraIII 246 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
402 CACTGTGTG
Hin1I 479 GGCGCC G[GA]^CG[CT]C
1061 GGCGTC
MflI 535 GGATCT [GA]^GATC[CT]
1307 GGATCC
MslI 174 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1098 CACCTTCGTG
NcoI 672 CCATGG C^CATGG
1203 CCATGG
XhoII 535 GGATCT [GA]^GATC[CT]
1307 GGATCC
XmnI 141 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1089 GAAGCGCTTC
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# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaI AvaII BalI BbvI
BcnI BglI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HincII HindII HpaII HphI
ItaI Ksp632I MaeII MboI MboII
MluNI MnlI MscI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
StyI TaqI TseI TspRI TthHB8I
XmaCI XmaI