Restriction map for
pF1KB0987
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AfaI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BalI BamHI BanII
BbeI BbrPI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstF5I BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FokI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeII
MamI MfeI MflI MluI MluNI
MroI MscI MseI MslI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Tth111I XbaI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 448 GTAGAC GT^[AC][GT]AC
AflIII 296 ACATGT A^C[GA][CT]GT
AlwI 3 GATCC GGATC(4/5)
Asp700I 745 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 514 GGTGCC G^G[CT][GA]CC
BbsI 148 GTCTTC GAAGAC(2/6)
BbvI 155 GCTGC GCAGC(8/12)
BfaI 193 CTAG C^TAG
BlpI 409 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 409 GCTCAGC GC^T[ACGT]AGC
BpuAI 148 GTCTTC GAAGAC(2/6)
BsgI 234 GTGCAG GTGCAG(16/14)
BsmAI 300 GTCTC GTCTC(1/5)
BspHI 306 TCATGA T^CATGA
BspLU11I 296 ACATGT A^CATGT
BspMI 236 GCAGGT ACCTGC(4/8)
BsrDI 384 CATTGC GCAATG(2/0)
BsrI 723 CCAGT ACTGG(1/-1)
CelII 409 GCTCAGC GC^T[ACGT]AGC
DsaI 453 CCACGG C^C[GA][CT]GG
EcoNI 60 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 638 CCAAGG C^C[AT][AT]GG
HphI 673 GGTGA GGTGA(8/7)
MaeI 193 CTAG C^TAG
PflMI 462 CCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 306 TCATGA T^CATGA
SmlI 403 CTCAAG C^T[CT][GA]AG
StuI 131 AGGCCT AGG^CCT
StyI 638 CCAAGG C^C[AT][AT]GG
TaqI 102 TCGA T^CGA
TfiI 572 GAATC G^A[AT]TC
TseI 155 GCTGC G^C[AT]GC
Tsp45I 674 GTGAC ^GT[GC]AC
TthHB8I 102 TCGA T^CGA
Van91I 462 CCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 500 CCAGGGAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 745 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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BsmFI 196 GGGAC GGGAC(10/14)
285 GTCCC
BsmI 274 GAATGC GAATGC(1/-1)
666 GAATGC
BstAPI 77 GCACCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
429 GCAGGCTCTGC
Cfr13I 478 GGGCC G^G[ACGT]CC
537 GGGCC
DpnII 3 GATC ^GATC
622 GATC
EaeI 566 CGGCCA [CT]^GGCC[GA]
635 CGGCCA
EarI 94 CTCTTC CTCTTC(1/4)
225 GAAGAG
Ksp632I 94 CTCTTC CTCTTC(1/4)
225 GAAGAG
MaeIII 595 GTAAC ^GT[ACGT]AC
674 GTGAC
MboI 3 GATC ^GATC
622 GATC
NdeII 3 GATC ^GATC
622 GATC
NlaIV 458 GGAACC GG[ACGT]^[ACGT]CC
514 GGTGCC
PleI 350 GACTC GAGTC(4/5)
737 GAGTC
PstI 427 CTGCAG CTGCA^G
436 CTGCAG
PvuII 508 CAGCTG CAG^CTG
541 CAGCTG
Sau3AI 3 GATC ^GATC
622 GATC
Sau96I 478 GGGCC G^G[ACGT]CC
537 GGGCC
SfcI 427 CTGCAG C^T[GA][CT]AG
436 CTGCAG
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AluI AlwNI AspHI BbvCI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI Bsp1286I BssKI BstNI
Cac8I DdeI EcoRII Fnu4HI HaeIII
HapII HinfI HpaII ItaI MboII
MnlI MspA1I MspI MvaI MwoI
NlaIII NspI ScrFI Tsp509I TspRI