Restriction map for
pF1KB6882
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# NO CUT ENZYMES ( 160 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvrII
BamHI BanII BbeI BbrPI BbsI
BbvCI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaHI BsaWI BseAI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstXI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DdeI DraI
DraIII Eam1105I EarI Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FokI FseI HgaI
Hin1I HindIII HpaI HphI KasI
KpnI Ksp632I KspI MaeI MamI
MfeI MflI MluI MroI MseI
MslI MunI NaeI NarI NdeI
NgoMIV NheI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SapI SbfI
SfaNI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tsp45I Tsp509I Tth111I Van91I
XbaI XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccI 373 GTCGAC GT^[AC][GT]AC
AlwI 368 GGATC GGATC(4/5)
AviII 262 TGCGCA TGC^GCA
BmyI 276 GGGCAC G[AGT]GC[ACT]^C
BpmI 343 CTCCAG CTGGAG(16/14)
BsaAI 390 CACGTA [CT]AC^GT[GA]
BsaI 199 GAGACC GGTCTC(1/5)
BseRI 523 GAGGAG GAGGAG(10/8)
BsgI 400 GTGCAG GTGCAG(16/14)
BsiEI 45 CGGCCG CG[GA][CT]^CG
BsiYI 81 CCGAGTCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 81 CCGAGTCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 199 GAGAC GTCTC(1/5)
Bsp1286I 276 GGGCAC G[AGT]GC[ACT]^C
BsrFI 202 ACCGGC [GA]^CCGG[CT]
Cfr10I 202 ACCGGC [GA]^CCGG[CT]
DrdI 169 GACAACATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 45 CGGCCG C^GGCCG
EclXI 45 CGGCCG C^GGCCG
Eco52I 45 CGGCCG C^GGCCG
FspI 262 TGCGCA TGC^GCA
HincII 373 GTCGAC GT[CT]^[GA]AC
HindII 373 GTCGAC GT[CT]^[GA]AC
MaeIII 569 GTTAC ^GT[ACGT]AC
MboII 501 GAAGA GAAGA(8/7)
NcoI 131 CCATGG C^CATGG
NotI 44 GCGGCCGC GC^GGCCGC
PstI 163 CTGCAG CTGCA^G
SalI 373 GTCGAC G^TCGAC
SexAI 121 ACCTGGT A^CC[AT]GGT
SfcI 163 CTGCAG C^T[GA][CT]AG
SmaI 467 CCCGGG CCC^GGG
TspRI 364 CACTG CA[GC]TG[ACGT][ACGT]^
XmaCI 467 CCCGGG C^CCGGG
XmaI 467 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 257 ACGTGT A^C[GA][CT]GT
389 ACACGT
AvaI 148 CCCGAG C^[CT]CG[GA]G
467 CCCGGG
BalI 134 TGGCCA TGG^CCA
536 TGGCCA
BanI 277 GGCACC G^G[CT][GA]CC
433 GGCACC
BcgI 294 CGAGGACCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
387 CGACACGTATGC
BcnI 467 CCCGG CC^[GC]GG
468 CCGGG
BsoBI 148 CCCGAG C^[CT]CG[GA]G
467 CCCGGG
Cac8I 106 GCTCGC GC[ACGT]^[ACGT]GC
482 GCCTGC
DsaI 131 CCATGG C^C[GA][CT]GG
191 CCGTGG
EcoT14I 131 CCATGG C^C[AT][AT]GG
224 CCAAGG
HapII 203 CCGG C^CGG
468 CCGG
HinfI 83 GAGTC G^A[ACGT]TC
233 GAGTC
HpaII 203 CCGG C^CGG
468 CCGG
MaeII 257 ACGT A^CGT
391 ACGT
MluNI 134 TGGCCA TGG^CCA
536 TGGCCA
MscI 134 TGGCCA TGG^CCA
536 TGGCCA
MspA1I 42 CAGCGG C[AC]G^C[GT]G
514 CAGCGG
MspI 203 CCGG C^CGG
468 CCGG
NciI 467 CCCGG CC^[GC]GG
468 CCGGG
NlaIII 132 CATG CATG^
270 CATG
PleI 83 GAGTC GAGTC(4/5)
233 GAGTC
ScaI 320 AGTACT AGT^ACT
404 AGTACT
StyI 131 CCATGG C^C[AT][AT]GG
224 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AccII AciI AfaI AluI AvaII
BbvI BsaJI BsmFI BsrI BssKI
BstNI BstUI CfoI Cfr13I DpnII
DraII EaeI EcoO109I EcoRII Fnu4HI
HaeII HaeIII HhaI HinP1I ItaI
MboI MnlI MvaI MvnI MwoI
NdeII NlaIV PpuMI RsaI Sau3AI
Sau96I ScrFI TaqI TseI TthHB8I