Restriction map for
pF1KB0993
##################################
# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BanI BbeI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstYI BstZ17I Bsu36I CelII CpoI
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HaeII
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MfeI
MflI MluI MluNI MroI MscI
MslI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccII 21 CGCG CG^CG
AcsI 571 GAATTC [GA]^AATT[CT]
ApaI 90 GGGCCC GGGCC^C
ApoI 571 GAATTC [GA]^AATT[CT]
AspHI 829 GAGCTC G[AT]GC[AT]^C
BbrPI 589 CACGTG CAC^GTG
BbvCI 109 GCTGAGG CCTCAGC(-5/-2)
BcgI 459 CGAGACTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 81 CCCGG CC^[GC]GG
BpmI 254 CTGGAG CTGGAG(16/14)
BsaAI 589 CACGTG [CT]AC^GT[GA]
BsaBI 432 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 334 CTGCAC GTGCAG(16/14)
BsiHKAI 829 GAGCTC G[AT]GC[AT]^C
BsmFI 24 GTCCC GGGAC(10/14)
BspDI 359 ATCGAT AT^CGAT
BstUI 21 CGCG CG^CG
BstXI 611 CCAGTTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 581 GCGC GCG^C
ClaI 359 ATCGAT AT^CGAT
DpnII 528 GATC ^GATC
EcoNI 333 CCTGCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 571 GAATTC G^AATTC
HgaI 22 GCGTC GACGC(5/10)
HhaI 581 GCGC GCG^C
HinP1I 581 GCGC G^CGC
MaeII 590 ACGT A^CGT
MaeIII 295 GTCAC ^GT[ACGT]AC
MamI 432 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 528 GATC ^GATC
MseI 286 TTAA T^TAA
MvnI 21 CGCG CG^CG
NaeI 75 GCCGGC GCC^GGC
NciI 81 CCCGG CC^[GC]GG
NdeII 528 GATC ^GATC
NgoMIV 75 GCCGGC G^CCGGC
NlaIII 375 CATG CATG^
NspI 374 GCATGT [GA]CATG^[CT]
PleI 257 GAGTC GAGTC(4/5)
PmaCI 589 CACGTG CAC^GTG
PmlI 589 CACGTG CAC^GTG
PstI 467 CTGCAG CTGCA^G
PvuII 653 CAGCTG CAG^CTG
SacI 829 GAGCTC GAGCT^C
SapI 684 GAAGAGC GCTCTTC(1/4)
Sau3AI 528 GATC ^GATC
SexAI 404 ACCTGGT A^CC[AT]GGT
SfcI 467 CTGCAG C^T[GA][CT]AG
StuI 248 AGGCCT AGG^CCT
TfiI 4 GATTC G^A[AT]TC
Tru9I 286 TTAA T^TAA
Tsp45I 295 GTCAC ^GT[GC]AC
Tsp509I 572 AATT ^AATT
XcmI 243 CCAGAAGGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AfaI 409 GTAC GT^AC
540 GTAC
AvaI 93 CCCGAG C^[CT]CG[GA]G
772 CCCGAG
BbsI 559 GAAGAC GAAGAC(2/6)
32 GTCTTC
BpuAI 559 GAAGAC GAAGAC(2/6)
32 GTCTTC
BsoBI 93 CCCGAG C^[CT]CG[GA]G
772 CCCGAG
EarI 511 GAAGAG CTCTTC(1/4)
684 GAAGAG
FauI 19 CCCGC CCCGC(4/6)
26 CCCGC
HinfI 4 GATTC G^A[ACGT]TC
257 GAGTC
Ksp632I 511 GAAGAG CTCTTC(1/4)
684 GAAGAG
MspA1I 27 CCGCTG C[AC]G^C[GT]G
653 CAGCTG
RsaI 409 GTAC GT^AC
540 GTAC
SmlI 484 CTCAAG C^T[CT][GA]AG
754 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AluI AvaII BanII BbvI
BmyI BsaI BsaJI BseRI BsiYI
BslI BsmAI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI Cac8I Cfr10I
Cfr13I DdeI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HpaII HphI
ItaI MboII MnlI MspI MvaI
MwoI NlaIV Sau96I ScrFI SfaNI
StyI TaqI TseI TspRI TthHB8I