Restriction map for
pF1KB0995
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# NO CUT ENZYMES ( 167 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AgeI AhdI AlwNI
AocI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsiEI BsiWI BsoBI Bsp1407I BspDI
BspHI BspMI BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeIII HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MaeII MamI MluI MluNI MscI
MspA1I MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccIII 832 TCCGGA T^CCGGA
AcsI 349 GAATTT [GA]^AATT[CT]
AfaI 61 GTAC GT^AC
AflIII 91 ACATGT A^C[GA][CT]GT
AluI 539 AGCT AG^CT
AlwI 159 GGATC GGATC(4/5)
ApoI 349 GAATTT [GA]^AATT[CT]
AvaII 320 GGACC G^G[AT]CC
BanI 700 GGTGCC G^G[CT][GA]CC
BbvI 244 GCTGC GCAGC(8/12)
BglII 582 AGATCT A^GATCT
BsaI 691 GGTCTC GGTCTC(1/5)
BsaWI 832 TCCGGA [AT]^CCGG[AT]
BseAI 832 TCCGGA T^CCGGA
BseRI 294 CTCCTC GAGGAG(10/8)
BsgI 30 CTGCAC GTGCAG(16/14)
BsmBI 611 GAGACG CGTCTC(1/5)
BsmFI 412 GGGAC GGGAC(10/14)
BspEI 832 TCCGGA T^CCGGA
BspLU11I 91 ACATGT A^CATGT
BsrFI 176 ACCGGC [GA]^CCGG[CT]
BssKI 331 CCTGG ^CC[ACGT]GG
BstNI 331 CCTGG CC^[AT]GG
BstXI 819 CCAAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 260 GCCTGC GC[ACGT]^[ACGT]GC
CfoI 258 GCGC GCG^C
Cfr10I 176 ACCGGC [GA]^CCGG[CT]
Cfr13I 320 GGACC G^G[ACGT]CC
DdeI 155 CTAAG C^T[ACGT]AG
DraIII 270 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRII 331 CCTGG ^CC[AT]GG
Fnu4HI 244 GCTGC GC^[ACGT]GC
HaeII 257 AGCGCC [GA]GCGC^[CT]
HhaI 258 GCGC GCG^C
HinP1I 258 GCGC G^CGC
HphI 504 GGTGA GGTGA(8/7)
ItaI 244 GCTGC GC^[ACGT]GC
MfeI 140 CAATTG C^AATTG
MroI 832 TCCGGA T^CCGGA
MunI 140 CAATTG C^AATTG
MvaI 331 CCTGG CC^[AT]GG
NspI 91 ACATGT [GA]CATG^[CT]
RsaI 61 GTAC GT^AC
Sau96I 320 GGACC G^G[ACGT]CC
ScrFI 331 CCTGG CC^[ACGT]GG
TseI 244 GCTGC G^C[AT]GC
Tsp45I 533 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AciI 339 CCGC CCGC(-3/-1)
755 GCGG
Alw44I 204 GTGCAC G^TGCAC
267 GTGCAC
ApaLI 204 GTGCAC G^TGCAC
267 GTGCAC
BsaJI 248 CCAAGG C^C[ACGT][ACGT]GG
773 CCTTGG
BsmI 127 GAATGC GAATGC(1/-1)
742 GAATGC
BstYI 159 GGATCT [GA]^GATC[CT]
582 AGATCT
EcoT14I 248 CCAAGG C^C[AT][AT]GG
773 CCTTGG
FauI 338 CCCGC CCCGC(4/6)
755 GCGGG
HapII 177 CCGG C^CGG
833 CCGG
HinfI 145 GAATC G^A[ACGT]TC
151 GATTC
HpaII 177 CCGG C^CGG
833 CCGG
MflI 159 GGATCT [GA]^GATC[CT]
582 AGATCT
MseI 353 TTAA T^TAA
749 TTAA
MslI 181 CACTGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
646 CATCTTTATG
MspI 177 CCGG C^CGG
833 CCGG
SfaNI 194 GATGC GCATC(5/9)
37 GCATC
SfcI 407 CTATAG C^T[GA][CT]AG
573 CTGTAG
StyI 248 CCAAGG C^C[AT][AT]GG
773 CCTTGG
TfiI 145 GAATC G^A[AT]TC
151 GATTC
Tru9I 353 TTAA T^TAA
749 TTAA
XhoII 159 GGATCT [GA]^GATC[CT]
582 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AspHI BmyI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrI BstAPI BstF5I
DpnII FokI MaeIII MboI MboII
MnlI MwoI NdeII NlaIII NlaIV
Sau3AI Tsp509I TspRI