Restriction map for
pF1KB0996
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# NO CUT ENZYMES ( 148 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BbeI BbrPI BbvCI BcgI BclI
BcnI BfaI BfrI BglI BglII
BlnI BsaAI BsaBI BsaHI BsaI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrFI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Cfr10I ClaI CpoI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI Hin1I
HincII HindII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 629 GTAGAC GT^[AC][GT]AC
AciI 348 GCGG CCGC(-3/-1)
AcsI 1238 GAATTT [GA]^AATT[CT]
ApoI 1238 GAATTT [GA]^AATT[CT]
Asp700I 910 GAACTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 1148 GGACC G^G[AT]CC
BamHI 888 GGATCC G^GATCC
BanI 351 GGCACC G^G[CT][GA]CC
BlpI 716 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 716 GCTCAGC GC^T[ACGT]AGC
BsgI 1102 GTGCAG GTGCAG(16/14)
BsmBI 256 GAGACG CGTCTC(1/5)
BspMI 43 ACCTGC ACCTGC(4/8)
BsrBI 346 GAGCGG CCGCTC(-3/-3)
BssSI 944 CTCGTG CACGAG(-5/-1)
BstAPI 1221 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 888 GGATCC [GA]^GATC[CT]
CelII 716 GCTCAGC GC^T[ACGT]AGC
CfoI 339 GCGC GCG^C
DraI 130 TTTAAA TTT^AAA
EcoT14I 800 CCTTGG C^C[AT][AT]GG
FauI 348 GCGGG CCCGC(4/6)
HaeII 338 GGCGCT [GA]GCGC^[CT]
HhaI 339 GCGC GCG^C
HinP1I 339 GCGC G^CGC
HindIII 832 AAGCTT A^AGCTT
MflI 888 GGATCC [GA]^GATC[CT]
MspA1I 226 CAGCTG C[AC]G^C[GT]G
NdeI 199 CATATG CA^TATG
PflMI 684 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 733 CTGCAG CTGCA^G
PvuII 226 CAGCTG CAG^CTG
SapI 663 GAAGAGC GCTCTTC(1/4)
SfaNI 83 GCATC GCATC(5/9)
SfcI 733 CTGCAG C^T[GA][CT]AG
SmlI 676 CTCAAG C^T[CT][GA]AG
SspI 1361 AATATT AAT^ATT
StyI 800 CCTTGG C^C[AT][AT]GG
TaqI 842 TCGA T^CGA
TthHB8I 842 TCGA T^CGA
Van91I 684 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 888 GGATCC [GA]^GATC[CT]
XmnI 910 GAACTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 461 GTAC GT^AC
514 GTAC
AocI 1028 CCTCAGG CC^T[ACGT]AGG
1152 CCTCAGG
BanII 188 GGGCTC G[GA]GC[CT]^C
714 GGGCTC
BciVI 949 GGATAC GTATCC(6/5)
1267 GTATCC
BsaWI 536 TCCGGT [AT]^CCGG[AT]
1069 ACCGGA
BsmFI 638 GGGAC GGGAC(10/14)
176 GTCCC
Bsu36I 1028 CCTCAGG CC^T[ACGT]AGG
1152 CCTCAGG
Cac8I 335 GCTGGC GC[ACGT]^[ACGT]GC
348 GCGGGC
Cfr13I 1148 GGACC G^G[ACGT]CC
1340 GGCCC
EarI 577 GAAGAG CTCTTC(1/4)
663 GAAGAG
Eco81I 1028 CCTCAGG CC^T[ACGT]AGG
1152 CCTCAGG
EcoNI 567 CCTACATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1381 CCTCCCCCAGG
HphI 1390 GGTGA GGTGA(8/7)
1025 TCACC
Ksp632I 577 GAAGAG CTCTTC(1/4)
663 GAAGAG
MaeII 392 ACGT A^CGT
1038 ACGT
RsaI 461 GTAC GT^AC
514 GTAC
Sau96I 1148 GGACC G^G[ACGT]CC
1340 GGCCC
XcmI 20 CCAAGAAGATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
218 CCAACAAGCAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AluI AlwI AlwNI BbsI BbvI
BmyI BpmI BpuAI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII ItaI MaeIII MboI
MboII MnlI MseI MslI MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI Sau3AI ScrFI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI