Restriction map for
pF1KB9971
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# NO CUT ENZYMES ( 111 enzymes) #
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AatII Acc65I AccIII AclI AgeI
AhdI Alw44I AlwI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbrPI BcgI BciVI BclI BglII
BlnI BsaAI BsaWI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstYI BstZ17I
Cfr10I ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
MaeII MfeI MflI MluI MroI
MunI NaeI NdeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacI SalI SbfI ScaI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I Van91I XbaI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 307 GTAGAC GT^[AC][GT]AC
AflII 1630 CTTAAG C^TTAAG
AflIII 596 ACATGT A^C[GA][CT]GT
AlwNI 625 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1026 CCTGAGG CC^T[ACGT]AGG
ApaI 232 GGGCCC GGGCC^C
BbeI 1105 GGCGCC GGCGC^C
BfaI 320 CTAG C^TAG
BfrI 1630 CTTAAG C^TTAAG
BsaBI 1260 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 202 GAGGAG GAGGAG(10/8)
BspHI 1256 TCATGA T^CATGA
BspLU11I 596 ACATGT A^CATGT
BsrBI 1145 CCGCTC CCGCTC(-3/-3)
BsrDI 1329 CATTGC GCAATG(2/0)
BstAPI 1473 GCAAGCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1026 CCTGAGG CC^T[ACGT]AGG
DraI 948 TTTAAA TTT^AAA
Eco81I 1026 CCTGAGG CC^T[ACGT]AGG
HincII 553 GTCAAC GT[CT]^[GA]AC
HindII 553 GTCAAC GT[CT]^[GA]AC
KasI 1105 GGCGCC G^GCGCC
MaeI 320 CTAG C^TAG
MamI 1260 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1105 GGCGCC GG^CGCC
NcoI 1214 CCATGG C^CATGG
NheI 319 GCTAGC G^CTAGC
NspI 596 ACATGT [GA]CATG^[CT]
PpuMI 354 GGGTCCC [GA]G^G[AT]CC[CT]
RcaI 1256 TCATGA T^CATGA
SexAI 416 ACCTGGT A^CC[AT]GGT
SfoI 1105 GGCGCC GGC^GCC
SmaI 1161 CCCGGG CCC^GGG
SmlI 1630 CTTAAG C^T[CT][GA]AG
TfiI 1322 GAATC G^A[AT]TC
XmaCI 1161 CCCGGG C^CCGGG
XmaI 1161 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 32 enzymes) #
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AcsI 676 GAATTT [GA]^AATT[CT]
1227 AAATTT
AcyI 1105 GGCGCC G[GA]^CG[CT]C
1579 GACGCC
ApoI 676 GAATTT [GA]^AATT[CT]
1227 AAATTT
AspHI 571 GTGCTC G[AT]GC[AT]^C
1075 GTGCTC
BanI 1105 GGCGCC G^G[CT][GA]CC
1657 GGCACC
BbsI 118 GTCTTC GAAGAC(2/6)
1450 GTCTTC
BbvCI 297 CCTCAGC CCTCAGC(-5/-2)
1458 GCTGAGG
BglI 362 GCCAGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
774 GCCTCACTGGC
BpuAI 118 GTCTTC GAAGAC(2/6)
1450 GTCTTC
BsaHI 1105 GGCGCC G[GA]^CG[CT]C
1579 GACGCC
BsaI 1543 GAGACC GGTCTC(1/5)
1567 GAGACC
BsiHKAI 571 GTGCTC G[AT]GC[AT]^C
1075 GTGCTC
BsmFI 356 GTCCC GGGAC(10/14)
852 GTCCC
BsmI 465 GAATGC GAATGC(1/-1)
1356 GAATGC
DpnII 711 GATC ^GATC
1344 GATC
EcoNI 618 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
963 CCTCGAAGAGG
HgaI 1527 GACGC GACGC(5/10)
1579 GACGC
Hin1I 1105 GGCGCC G[GA]^CG[CT]C
1579 GACGCC
KspI 236 CCGCGG CCGC^GG
1517 CCGCGG
MboI 711 GATC ^GATC
1344 GATC
NdeII 711 GATC ^GATC
1344 GATC
PstI 622 CTGCAG CTGCA^G
790 CTGCAG
PvuII 300 CAGCTG CAG^CTG
793 CAGCTG
SacII 236 CCGCGG CCGC^GG
1517 CCGCGG
SapI 392 GCTCTTC GCTCTTC(1/4)
609 GCTCTTC
Sau3AI 711 GATC ^GATC
1344 GATC
SfcI 622 CTGCAG C^T[GA][CT]AG
790 CTGCAG
StuI 225 AGGCCT AGG^CCT
245 AGGCCT
TaqI 965 TCGA T^CGA
1154 TCGA
Tsp509I 677 AATT ^AATT
1228 AATT
TthHB8I 965 TCGA T^CGA
1154 TCGA
XcmI 695 CCAGAAGAACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1187 CCATTGACCGCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AfaI AluI AvaI
AvaII BalI BanII BbvI BcnI
BlpI BmyI BpmI Bpu1102I BsaJI
BsgI BsiYI BslI BsmAI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstXI Cac8I CelII
CfoI Cfr13I DdeI DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeIII MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIII NlaIV PleI RsaI
Sau96I ScrFI SfaNI StyI Tru9I
TseI Tsp45I TspRI