Restriction map for
pF1KB7648
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI ApaI ApaLI
ApoI AscI AseI AsnI AspEI
AspHI AspI AviII AvrII BalI
BamHI BanII BbeI BbrPI BcgI
BciVI BclI BfrI BglII BlnI
BlpI BmyI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmAI BsmBI Bsp1286I
Bsp1407I BspDI BspEI BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstEII BstPI BstUI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoT22I FbaI FseI FspI
Hin1I HindIII HpaI KasI KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfcI SfiI SfoI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 1132 GGTACC G^GTACC
AflIII 1023 ACATGT A^C[GA][CT]GT
AlwI 8 GGATC GGATC(4/5)
Aor51HI 341 AGCGCT AGC^GCT
Asp700I 942 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1132 GGTACC G^GTACC
AvaI 683 CCCGAG C^[CT]CG[GA]G
BanI 1132 GGTACC G^G[CT][GA]CC
BbvCI 103 GCTGAGG CCTCAGC(-5/-2)
BbvI 739 GCTGC GCAGC(8/12)
BcnI 1087 CCGGG CC^[GC]GG
BglI 742 GCCAAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 628 CTGCAC GTGCAG(16/14)
BsmFI 1123 GGGAC GGGAC(10/14)
BsmI 335 GCATTC GAATGC(1/-1)
BsoBI 683 CCCGAG C^[CT]CG[GA]G
BspHI 711 TCATGA T^CATGA
BspLU11I 1023 ACATGT A^CATGT
BsrDI 490 CATTGC GCAATG(2/0)
BstAPI 751 GCAAATTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 329 TTCGAA TT^CGAA
BstYI 8 GGATCT [GA]^GATC[CT]
DraII 107 AGGACCT [GA]G^G[ACGT]CC[CT]
EarI 728 CTCTTC CTCTTC(1/4)
Eco47III 341 AGCGCT AGC^GCT
EcoO109I 107 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 595 GATATC GAT^ATC
HaeII 341 AGCGCT [GA]GCGC^[CT]
HapII 1087 CCGG C^CGG
HgaI 359 GACGC GACGC(5/10)
HincII 670 GTCAAC GT[CT]^[GA]AC
HindII 670 GTCAAC GT[CT]^[GA]AC
HpaII 1087 CCGG C^CGG
KpnI 1132 GGTACC GGTAC^C
Ksp632I 728 CTCTTC CTCTTC(1/4)
MaeIII 55 GTCAC ^GT[ACGT]AC
MflI 8 GGATCT [GA]^GATC[CT]
MslI 277 CATCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 719 CAGCTG C[AC]G^C[GT]G
MspI 1087 CCGG C^CGG
NciI 1087 CCGGG CC^[GC]GG
NcoI 1166 CCATGG C^CATGG
NdeI 665 CATATG CA^TATG
NspV 329 TTCGAA TT^CGAA
PpuMI 107 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 719 CAGCTG CAG^CTG
RcaI 711 TCATGA T^CATGA
SapI 727 GCTCTTC GCTCTTC(1/4)
SfuI 329 TTCGAA TT^CGAA
StuI 300 AGGCCT AGG^CCT
TseI 739 GCTGC G^C[AT]GC
Tsp45I 55 GTCAC ^GT[GC]AC
XcmI 236 CCAATGCCATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 8 GGATCT [GA]^GATC[CT]
XmnI 942 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BbsI 256 GTCTTC GAAGAC(2/6)
707 GTCTTC
BfaI 928 CTAG C^TAG
1058 CTAG
BpmI 550 CTGGAG CTGGAG(16/14)
733 CTGGAG
BpuAI 256 GTCTTC GAAGAC(2/6)
707 GTCTTC
BseRI 82 GAGGAG GAGGAG(10/8)
1069 GAGGAG
BstXI 308 CCAAGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
818 CCACACGACTGG
CfoI 188 GCGC GCG^C
342 GCGC
DsaI 803 CCGTGG C^C[GA][CT]GG
1166 CCATGG
EcoT14I 1031 CCAAGG C^C[AT][AT]GG
1166 CCATGG
FauI 45 CCCGC CCCGC(4/6)
96 CCCGC
Fnu4HI 153 GCGGC GC^[ACGT]GC
739 GCTGC
HhaI 188 GCGC GCG^C
342 GCGC
HinP1I 188 GCGC G^CGC
342 GCGC
HphI 56 TCACC GGTGA(8/7)
815 TCACC
ItaI 153 GCGGC GC^[ACGT]GC
739 GCTGC
MaeI 928 CTAG C^TAG
1058 CTAG
MseI 850 TTAA T^TAA
911 TTAA
NspI 404 GCATGT [GA]CATG^[CT]
1023 ACATGT
SfaNI 724 GATGC GCATC(5/9)
276 GCATC
StyI 1031 CCAAGG C^C[AT][AT]GG
1166 CCATGG
Tru9I 850 TTAA T^TAA
911 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AfaI AluI AvaII BsaJI
BsiYI BslI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII FokI HaeIII HinfI MboI
MboII MnlI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI TaqI TfiI Tsp509I
TspRI TthHB8I