Restriction map for
pF1KB7676
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AgeI AhdI AlwNI AocI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BalI BamHI
BanI BbeI BbvCI BcgI BciVI
BclI BfrI BlnI BsaBI BsaI
BsaWI BseAI BseRI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BsrI BssSI
Bst1107I BstBI BstEII BstF5I BstPI
BstZ17I Bsu36I ClaI DraI Eam1105I
EarI Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FokI FseI
HindIII HpaI KasI KpnI Ksp632I
MamI MfeI MluI MluNI MroI
MscI MunI NarI NcoI NdeI
NheI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PshBI Psp1406I PvuI PvuII RcaI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
TfiI Tsp509I Tth111I Van91I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AatII 1134 GACGTC GACGT^C
AfaI 528 GTAC GT^AC
AflIII 433 ACGTGT A^C[GA][CT]GT
AlwI 705 GGATC GGATC(4/5)
Aor51HI 338 AGCGCT AGC^GCT
AviII 806 TGCGCA TGC^GCA
BfaI 1112 CTAG C^TAG
BglII 176 AGATCT A^GATCT
BsmAI 115 GAGAC GTCTC(1/5)
BsmBI 115 GAGACG CGTCTC(1/5)
BspMI 194 ACCTGC ACCTGC(4/8)
BsrFI 160 GCCGGC [GA]^CCGG[CT]
BssHII 387 GCGCGC G^CGCGC
BstAPI 296 GCATCTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 617 CCAACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 160 GCCGGC [GA]^CCGG[CT]
CpoI 182 CGGACCG CG^G[AT]CCG
DraIII 429 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 338 AGCGCT AGC^GCT
EcoT14I 878 CCAAGG C^C[AT][AT]GG
FspI 806 TGCGCA TGC^GCA
HincII 1005 GTCGAC GT[CT]^[GA]AC
HindII 1005 GTCGAC GT[CT]^[GA]AC
MaeI 1112 CTAG C^TAG
MslI 990 CACGGGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 160 GCCGGC GCC^GGC
NgoMIV 160 GCCGGC G^CCGGC
NotI 565 GCGGCCGC GC^GGCCGC
PpuMI 923 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1134 GACGTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 528 GTAC GT^AC
RsrII 182 CGGACCG CG^G[AT]CCG
SalI 1005 GTCGAC G^TCGAC
SmaI 690 CCCGGG CCC^GGG
SmlI 232 CTCAAG C^T[CT][GA]AG
SphI 803 GCATGC GCATG^C
StyI 878 CCAAGG C^C[AT][AT]GG
XmaCI 690 CCCGGG C^CCGGG
XmaI 690 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 487 GTCTAC GT^[AC][GT]AC
1005 GTCGAC
Alw44I 31 GTGCAC G^TGCAC
426 GTGCAC
ApaLI 31 GTGCAC G^TGCAC
426 GTGCAC
BbrPI 423 CACGTG CAC^GTG
432 CACGTG
BlpI 729 GCTAAGC GC^T[ACGT]AGC
1071 GCTCAGC
Bpu1102I 729 GCTAAGC GC^T[ACGT]AGC
1071 GCTCAGC
BsaAI 423 CACGTG [CT]AC^GT[GA]
432 CACGTG
BsgI 334 GTGCAG GTGCAG(16/14)
658 GTGCAG
BsmFI 395 GGGAC GGGAC(10/14)
1030 GGGAC
BstYI 176 AGATCT [GA]^GATC[CT]
705 GGATCT
CelII 729 GCTAAGC GC^T[ACGT]AGC
1071 GCTCAGC
DdeI 730 CTAAG C^T[ACGT]AG
1072 CTCAG
DpnII 177 GATC ^GATC
706 GATC
EagI 566 CGGCCG C^GGCCG
670 CGGCCG
EclXI 566 CGGCCG C^GGCCG
670 CGGCCG
Eco52I 566 CGGCCG C^GGCCG
670 CGGCCG
HinfI 262 GAGTC G^A[ACGT]TC
1200 GACTC
KspI 102 CCGCGG CCGC^GG
578 CCGCGG
MboI 177 GATC ^GATC
706 GATC
MflI 176 AGATCT [GA]^GATC[CT]
705 GGATCT
MseI 860 TTAA T^TAA
1144 TTAA
NdeII 177 GATC ^GATC
706 GATC
NspI 221 ACATGC [GA]CATG^[CT]
803 GCATGC
PleI 1200 GACTC GAGTC(4/5)
262 GAGTC
PmaCI 423 CACGTG CAC^GTG
432 CACGTG
PmlI 423 CACGTG CAC^GTG
432 CACGTG
SacI 268 GAGCTC GAGCT^C
1054 GAGCTC
SacII 102 CCGCGG CCGC^GG
578 CCGCGG
Sau3AI 177 GATC ^GATC
706 GATC
Tru9I 860 TTAA T^TAA
1144 TTAA
XhoII 176 AGATCT [GA]^GATC[CT]
705 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AcyI AluI ApaI
AspHI AvaI AvaII BanII BbsI
BbvI BcnI BglI BmyI BpmI
BpuAI BsaHI BsaJI BsiEI BsiHKAI
BsiYI BslI BsoBI Bsp1286I BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DraII DrdI DsaI EaeI EcoO109I
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII HphI ItaI MaeII MaeIII
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NlaIII NlaIV
PstI Sau96I ScrFI SfaNI SfcI
TaqI TseI Tsp45I TspRI TthHB8I