Restriction map for
pF1KB8471
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# NO CUT ENZYMES ( 93 enzymes) #
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AccI AccIII AclI AflII AgeI
AhdI AlwI Aor51HI AscI AseI
AsnI AspEI AspI AviII AvrII
BamHI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BsaBI BsaWI BseAI BsiWI BspDI
BspEI BssHII BssSI Bst1107I BstBI
BstYI BstZ17I ClaI CpoI DraI
DraIII DrdI Eam1105I Eco47III EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KspI MaeI MamI
MfeI MflI MluI MroI MunI
NdeI NheI NotI NsiI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI ScaI
SexAI SfuI SgrAI SnaBI SpeI
SphI SspI StuI SwaI Tth111I
Van91I XbaI XhoII
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# ONE CUT ENZYMES ( 41 enzymes) #
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Acc65I 1768 GGTACC G^GTACC
Alw44I 2580 GTGCAC G^TGCAC
ApaLI 2580 GTGCAC G^TGCAC
Asp700I 1143 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1768 GGTACC G^GTACC
BbeI 247 GGCGCC GGCGC^C
BlpI 1038 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1038 GCTGAGC GC^T[ACGT]AGC
BsaAI 1071 CACGTA [CT]AC^GT[GA]
BsiEI 858 CGGCCG CG[GA][CT]^CG
Bsp1407I 1304 TGTACA T^GTACA
BspHI 2552 TCATGA T^CATGA
BsrGI 1304 TGTACA T^GTACA
BstEII 2386 GGTAACC G^GT[ACGT]ACC
BstPI 2386 GGTAACC G^GT[ACGT]ACC
CelII 1038 GCTGAGC GC^T[ACGT]AGC
EagI 858 CGGCCG C^GGCCG
EclXI 858 CGGCCG C^GGCCG
Eco52I 858 CGGCCG C^GGCCG
EcoNI 2166 CCTCTACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2386 GGTAACC G^GT[ACGT]ACC
HgaI 2066 GCGTC GACGC(5/10)
HincII 2146 GTCAAC GT[CT]^[GA]AC
HindII 2146 GTCAAC GT[CT]^[GA]AC
KasI 247 GGCGCC G^GCGCC
KpnI 1768 GGTACC GGTAC^C
MseI 1727 TTAA T^TAA
NarI 247 GGCGCC GG^CGCC
NruI 2308 TCGCGA TCG^CGA
PaeR7I 2317 CTCGAG C^TCGAG
RcaI 2552 TCATGA T^CATGA
SacI 1041 GAGCTC GAGCT^C
SbfI 1116 CCTGCAGG CCTGCA^GG
SfiI 1252 GGCCCTAAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 247 GGCGCC GGC^GCC
Sse8387I 1116 CCTGCAGG CCTGCA^GG
SspBI 1304 TGTACA T^GTACA
Tru9I 1727 TTAA T^TAA
XcmI 266 CCAATGGGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2317 CTCGAG C^TCGAG
XmnI 1143 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AatII 2056 GACGTC GACGT^C
2626 GACGTC
AcsI 1003 AAATTC [GA]^AATT[CT]
1846 GAATTT
AflIII 1313 ACATGT A^C[GA][CT]GT
1751 ACATGT
AlwNI 2121 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
2656 CAGAGACTG
AocI 1255 CCTAAGG CC^T[ACGT]AGG
1327 CCTGAGG
ApoI 1003 AAATTC [GA]^AATT[CT]
1846 GAATTT
BbsI 1732 GAAGAC GAAGAC(2/6)
2548 GTCTTC
BglI 1253 GCCCTAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1803 GCCCTCCCGGC
BpuAI 1732 GAAGAC GAAGAC(2/6)
2548 GTCTTC
BsaI 367 GAGACC GGTCTC(1/5)
463 GAGACC
BsmBI 2628 CGTCTC CGTCTC(1/5)
995 GAGACG
BspLU11I 1313 ACATGT A^CATGT
1751 ACATGT
BspMI 1119 GCAGGT ACCTGC(4/8)
1664 GCAGGT
BsrBI 52 GAGCGG CCGCTC(-3/-3)
1429 GAGCGG
Bsu36I 1255 CCTAAGG CC^T[ACGT]AGG
1327 CCTGAGG
Eco81I 1255 CCTAAGG CC^T[ACGT]AGG
1327 CCTGAGG
HaeII 110 AGCGCC [GA]GCGC^[CT]
247 GGCGCC
NaeI 503 GCCGGC GCC^GGC
2183 GCCGGC
NcoI 230 CCATGG C^CATGG
2279 CCATGG
NgoMIV 503 GCCGGC G^CCGGC
2183 GCCGGC
NspI 1313 ACATGT [GA]CATG^[CT]
1751 ACATGT
SapI 1374 GAAGAGC GCTCTTC(1/4)
2187 GCTCTTC
SmlI 384 CTCAAG C^T[CT][GA]AG
2317 CTCGAG
Tsp509I 1004 AATT ^AATT
1847 AATT
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# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
ApaI AspHI AvaI AvaII BalI
BanI BanII BbvI BcnI BmyI
BpmI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BsrDI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI StyI TaqI TfiI TseI
Tsp45I TspRI TthHB8I XmaCI XmaI