Restriction map for
pF1KB8508
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII AccI AccIII AclI AcyI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I AspEI AviII AvrII BalI
BbeI BbrPI BciVI BclI BfrI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BseAI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII Bst1107I
BstBI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV EcoT22I FbaI FseI FspI
HaeII Hin1I HpaI KasI KspI
MaeII MamI MfeI MluNI MroI
MscI MspA1I MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SspBI SspI SwaI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AflIII 1369 ACGCGT A^C[GA][CT]GT
AocI 2556 CCTCAGG CC^T[ACGT]AGG
AspI 1629 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1960 GGATCC G^GATCC
BbsI 172 GAAGAC GAAGAC(2/6)
BbvCI 353 CCTCAGC CCTCAGC(-5/-2)
BcgI 541 CGAGGGTCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 191 GCCCTTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 576 AGATCT A^GATCT
BpuAI 172 GAAGAC GAAGAC(2/6)
BsgI 2396 GTGCAG GTGCAG(16/14)
BsmBI 844 GAGACG CGTCTC(1/5)
BspHI 1836 TCATGA T^CATGA
BstAPI 49 GCATGGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2556 CCTCAGG CC^T[ACGT]AGG
CfoI 426 GCGC GCG^C
DraII 2581 AGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 1716 CCGTGG C^C[GA][CT]GG
Eco81I 2556 CCTCAGG CC^T[ACGT]AGG
EcoO109I 2581 AGGCCCC [GA]G^G[ACGT]CC[CT]
FauI 2284 GCGGG CCCGC(4/6)
HgaI 1371 GCGTC GACGC(5/10)
HhaI 426 GCGC GCG^C
HinP1I 426 GCGC G^CGC
HincII 312 GTTGAC GT[CT]^[GA]AC
HindII 312 GTTGAC GT[CT]^[GA]AC
HindIII 2204 AAGCTT A^AGCTT
MluI 1369 ACGCGT A^CGCGT
MslI 1537 CACCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PaeR7I 809 CTCGAG C^TCGAG
PflFI 1629 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1192 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1836 TCATGA T^CATGA
SmlI 809 CTCGAG C^T[CT][GA]AG
SpeI 1750 ACTAGT A^CTAGT
StuI 1210 AGGCCT AGG^CCT
Tth111I 1629 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1192 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 643 TCTAGA T^CTAGA
XcmI 317 CCACTAATATAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 809 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 21 enzymes) #
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Acc65I 729 GGTACC G^GTACC
1924 GGTACC
Asp718I 729 GGTACC G^GTACC
1924 GGTACC
AvaI 809 CTCGAG C^[CT]CG[GA]G
1474 CCCGAG
BpmI 505 CTGGAG CTGGAG(16/14)
944 CTGGAG
BsaI 786 GAGACC GGTCTC(1/5)
1996 GAGACC
BsoBI 809 CTCGAG C^[CT]CG[GA]G
1474 CCCGAG
BssSI 1758 CACGAG CACGAG(-5/-1)
566 CTCGTG
BstEII 1344 GGTCACC G^GT[ACGT]ACC
1446 GGTGACC
BstPI 1344 GGTCACC G^GT[ACGT]ACC
1446 GGTGACC
BstXI 1038 CCAAAACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1436 CCAAAGAAGTGG
EarI 1240 GAAGAG CTCTTC(1/4)
2386 GAAGAG
EcoNI 1603 CCTATGTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2360 CCTTTGACAGG
EcoO65I 1344 GGTCACC G^GT[ACGT]ACC
1446 GGTGACC
EcoRI 1705 GAATTC G^AATTC
2116 GAATTC
EcoT14I 109 CCAAGG C^C[AT][AT]GG
2485 CCAAGG
KpnI 729 GGTACC GGTAC^C
1924 GGTACC
Ksp632I 1240 GAAGAG CTCTTC(1/4)
2386 GAAGAG
NspI 758 ACATGC [GA]CATG^[CT]
2345 ACATGC
PleI 1777 GACTC GAGTC(4/5)
1633 GAGTC
SexAI 789 ACCTGGT A^CC[AT]GGT
1059 ACCTGGT
StyI 109 CCAAGG C^C[AT][AT]GG
2485 CCAAGG
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# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
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AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BanI BanII BbvI BcnI BfaI
BmyI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MflI
MnlI MseI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PstI RsaI SacI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII