##################################
# NO CUT ENZYMES (  210 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BbvI        BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaI        BsaJI       BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiWI       BsiYI       BslI        
BsmFI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BsrI        BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      ClaI        CpoI        DpnII       DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoT14I     EcoT22I     
FauI        FbaI        Fnu4HI      FseI        FspI        
HaeII       HapII       HhaI        HinP1I      HincII      
HindII      HpaI        HpaII       HphI        ItaI        
KasI        KpnI        KspI        MaeII       MamI        
MboI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MspA1I      MspI        MunI        
MvnI        MwoI        NaeI        NarI        NciI        
NcoI        NdeI        NdeII       NgoMIV      NheI        
NlaIV       NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        Sau3AI      Sau96I      
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TseI        
Tsp45I      Tth111I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AciI           49  GCGG                 CCGC(-3/-1)
AcyI          232  GGCGTC               G[GA]^CG[CT]C
AfaI          163  GTAC                 GT^AC
AluI           86  AGCT                 AG^CT
AspHI          33  GTGCTC               G[AT]GC[AT]^C
BmyI           33  GTGCTC               G[AGT]GC[ACT]^C
BsaHI         232  GGCGTC               G[GA]^CG[CT]C
BsiHKAI        33  GTGCTC               G[AT]GC[AT]^C
BsmBI         234  CGTCTC               CGTCTC(1/5)
Bsp1286I       33  GTGCTC               G[AGT]GC[ACT]^C
BssKI         339  CCAGG                ^CC[ACGT]GG
BstNI         339  CCAGG                CC^[AT]GG
DraI          214  TTTAAA               TTT^AAA
EcoRII        339  CCAGG                ^CC[AT]GG
EcoRV         481  GATATC               GAT^ATC
HaeIII        475  GGCC                 GG^CC
HgaI          233  GCGTC                GACGC(5/10)
Hin1I         232  GGCGTC               G[GA]^CG[CT]C
HindIII        85  AAGCTT               A^AGCTT
HinfI         153  GAATC                G^A[ACGT]TC
MslI           40  CACTTTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI          339  CCAGG                CC^[AT]GG
RsaI          163  GTAC                 GT^AC
ScrFI         339  CCAGG                CC^[ACGT]GG
SfaNI          46  GATGC                GCATC(5/9)
TaqI          185  TCGA                 T^CGA
TfiI          153  GAATC                G^A[AT]TC
TspRI         246  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       185  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AccI          208  GTAGAC               GT^[AC][GT]AC
              397  GTAGAC              
AcsI          220  GAATTC               [GA]^AATT[CT]
              454  GAATTC              
ApoI          220  GAATTC               [GA]^AATT[CT]
              454  GAATTC              
BfaI           67  CTAG                 C^TAG
              478  CTAG                
BsmAI         132  GTCTC                GTCTC(1/5)
              235  GTCTC               
BstF5I         51  GGATG                GGATG(2/0)
              426  GGATG               
DdeI          140  CTGAG                C^T[ACGT]AG
              239  CTCAG               
EarI          334  CTCTTC               CTCTTC(1/4)
              121  GAAGAG              
EcoRI         220  GAATTC               G^AATTC
              454  GAATTC              
FokI           51  GGATG                GGATG(9/13)
              426  GGATG               
Ksp632I       334  CTCTTC               CTCTTC(1/4)
              121  GAAGAG              
MaeI           67  CTAG                 C^TAG
              478  CTAG                
MaeIII        144  GTTAC                ^GT[ACGT]AC
              384  GTTAC               
NlaIII        129  CATG                 CATG^
              300  CATG                


#########################################
# 3 OR MORE CUT ENZYMES (    5 enzymes) #
#########################################

MboII       MnlI        MseI        Tru9I       Tsp509I