################################## # NO CUT ENZYMES ( 210 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsiYI BslI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DpnII DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoT14I EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII HapII HhaI HinP1I HincII HindII HpaI HpaII HphI ItaI KasI KpnI KspI MaeII MamI MboI MfeI MflI MluI MluNI MroI MscI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI Sau96I SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TseI Tsp45I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### AciI 49 GCGG CCGC(-3/-1) AcyI 232 GGCGTC G[GA]^CG[CT]C AfaI 163 GTAC GT^AC AluI 86 AGCT AG^CT AspHI 33 GTGCTC G[AT]GC[AT]^C BmyI 33 GTGCTC G[AGT]GC[ACT]^C BsaHI 232 GGCGTC G[GA]^CG[CT]C BsiHKAI 33 GTGCTC G[AT]GC[AT]^C BsmBI 234 CGTCTC CGTCTC(1/5) Bsp1286I 33 GTGCTC G[AGT]GC[ACT]^C BssKI 339 CCAGG ^CC[ACGT]GG BstNI 339 CCAGG CC^[AT]GG DraI 214 TTTAAA TTT^AAA EcoRII 339 CCAGG ^CC[AT]GG EcoRV 481 GATATC GAT^ATC HaeIII 475 GGCC GG^CC HgaI 233 GCGTC GACGC(5/10) Hin1I 232 GGCGTC G[GA]^CG[CT]C HindIII 85 AAGCTT A^AGCTT HinfI 153 GAATC G^A[ACGT]TC MslI 40 CACTTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvaI 339 CCAGG CC^[AT]GG RsaI 163 GTAC GT^AC ScrFI 339 CCAGG CC^[ACGT]GG SfaNI 46 GATGC GCATC(5/9) TaqI 185 TCGA T^CGA TfiI 153 GAATC G^A[AT]TC TspRI 246 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 185 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AccI 208 GTAGAC GT^[AC][GT]AC 397 GTAGAC AcsI 220 GAATTC [GA]^AATT[CT] 454 GAATTC ApoI 220 GAATTC [GA]^AATT[CT] 454 GAATTC BfaI 67 CTAG C^TAG 478 CTAG BsmAI 132 GTCTC GTCTC(1/5) 235 GTCTC BstF5I 51 GGATG GGATG(2/0) 426 GGATG DdeI 140 CTGAG C^T[ACGT]AG 239 CTCAG EarI 334 CTCTTC CTCTTC(1/4) 121 GAAGAG EcoRI 220 GAATTC G^AATTC 454 GAATTC FokI 51 GGATG GGATG(9/13) 426 GGATG Ksp632I 334 CTCTTC CTCTTC(1/4) 121 GAAGAG MaeI 67 CTAG C^TAG 478 CTAG MaeIII 144 GTTAC ^GT[ACGT]AC 384 GTTAC NlaIII 129 CATG CATG^ 300 CATG ######################################### # 3 OR MORE CUT ENZYMES ( 5 enzymes) # ######################################### MboII MnlI MseI Tru9I Tsp509I