Restriction map for
pF1KE0632
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# NO CUT ENZYMES ( 124 enzymes) #
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AccI AccIII AclI AflII AflIII
AgeI AhdI AlwI AocI Aor51HI
AseI AsnI Asp700I AspEI AviII
AvrII BalI BamHI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BsaAI BsaBI BsaWI
BseAI BsiWI BsmI Bsp1407I BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CpoI
DraI DraIII Eam1105I EarI Eco47III
Eco81I EcoNI EcoO65I EcoRV EcoT14I
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI Ksp632I MamI
MfeI MflI MluI MluNI MroI
MscI MseI MunI NaeI NcoI
NdeI NgoMIV NheI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PleI PmaCI PmeI PmlI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SalI SapI SbfI
ScaI SfiI SfuI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tru9I Van91I
XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 898 GACGTC GACGT^C
Acc65I 594 GGTACC G^GTACC
AcsI 82 GAATTC [GA]^AATT[CT]
AfaI 595 GTAC GT^AC
ApaI 1111 GGGCCC GGGCC^C
ApoI 82 GAATTC [GA]^AATT[CT]
AscI 949 GGCGCGCC GG^CGCGCC
Asp718I 594 GGTACC G^GTACC
BbeI 529 GGCGCC GGCGC^C
BfaI 254 CTAG C^TAG
BlpI 1044 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1044 GCTCAGC GC^T[ACGT]AGC
BsaI 206 GAGACC GGTCTC(1/5)
BsiEI 777 CGGCCG CG[GA][CT]^CG
BsmBI 460 GAGACG CGTCTC(1/5)
BspDI 439 ATCGAT AT^CGAT
BspMI 209 ACCTGC ACCTGC(4/8)
BsrI 918 ACTGG ACTGG(1/-1)
BstAPI 996 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1044 GCTCAGC GC^T[ACGT]AGC
ClaI 439 ATCGAT AT^CGAT
DpnII 708 GATC ^GATC
DrdI 892 GACAACGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 777 CGGCCG C^GGCCG
EclXI 777 CGGCCG C^GGCCG
Eco52I 777 CGGCCG C^GGCCG
EcoRI 82 GAATTC G^AATTC
HinfI 35 GATTC G^A[ACGT]TC
KasI 529 GGCGCC G^GCGCC
KpnI 594 GGTACC GGTAC^C
MaeI 254 CTAG C^TAG
MboI 708 GATC ^GATC
NarI 529 GGCGCC GG^CGCC
NdeII 708 GATC ^GATC
NotI 776 GCGGCCGC GC^GGCCGC
PshAI 1063 GACCCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 595 GTAC GT^AC
Sau3AI 708 GATC ^GATC
SfaNI 575 GCATC GCATC(5/9)
SfoI 529 GGCGCC GGC^GCC
SgrAI 466 CACCGGCG C[GA]^CCGG[CT]G
TfiI 35 GATTC G^A[AT]TC
Tsp509I 83 AATT ^AATT
XcmI 434 CCATCATCGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 529 GGCGCC G[GA]^CG[CT]C
898 GACGTC
AlwNI 997 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1036 CAGCGGCTG
BanII 324 GGGCTC G[GA]GC[CT]^C
1111 GGGCCC
BbsI 678 GAAGAC GAAGAC(2/6)
277 GTCTTC
BpuAI 678 GAAGAC GAAGAC(2/6)
277 GTCTTC
BsaHI 529 GGCGCC G[GA]^CG[CT]C
898 GACGTC
BseRI 295 GAGGAG GAGGAG(10/8)
1102 GAGGAG
BsrFI 467 ACCGGC [GA]^CCGG[CT]
485 ACCGGC
BssHII 356 GCGCGC G^CGCGC
950 GCGCGC
Cfr10I 467 ACCGGC [GA]^CCGG[CT]
485 ACCGGC
DsaI 818 CCGCGG C^C[GA][CT]GG
1115 CCGCGG
EaeI 671 TGGCCG [CT]^GGCC[GA]
777 CGGCCG
Hin1I 529 GGCGCC G[GA]^CG[CT]C
898 GACGTC
HphI 654 GGTGA GGTGA(8/7)
734 TCACC
KspI 818 CCGCGG CCGC^GG
1115 CCGCGG
NlaIII 591 CATG CATG^
702 CATG
PstI 853 CTGCAG CTGCA^G
1003 CTGCAG
SacII 818 CCGCGG CCGC^GG
1115 CCGCGG
SexAI 449 ACCTGGT A^CC[AT]GGT
689 ACCAGGT
SfcI 853 CTGCAG C^T[GA][CT]AG
1003 CTGCAG
TaqI 440 TCGA T^CGA
908 TCGA
TthHB8I 440 TCGA T^CGA
908 TCGA
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AluI Alw44I ApaLI
AspHI AspI AvaI AvaII BanI
BbvI BcnI BmyI BpmI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DraII EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HpaII ItaI
MaeII MaeIII MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIV PflFI PpuMI Sau96I
ScrFI SmaI TseI Tsp45I TspRI
Tth111I XmaCI XmaI