Restriction map for
pF1KB0916
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII BamHI
BbrPI BbsI BbvCI BciVI BclI
BfrI BglI BglII BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BsgI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspHI BspMI BsrBI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoT22I FbaI FseI FspI
HindIII HpaI KpnI KspI MaeIII
MamI MluI MroI MseI MvnI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SapI ScaI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SphI SspBI SspI StuI SwaI
Tru9I Tsp45I Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 797 GTCGAC GT^[AC][GT]AC
AfaI 423 GTAC GT^AC
AlwNI 167 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 304 GGGCCC GGGCC^C
Asp700I 678 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 196 GGTCC G^G[AT]CC
AvrII 417 CCTAGG C^CTAGG
BalI 386 TGGCCA TGG^CCA
BbeI 292 GGCGCC GGCGC^C
BcgI 71 CGATGCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 417 CCTAGG C^CTAGG
BsmI 326 GCATTC GAATGC(1/-1)
BspLU11I 527 ACATGT A^CATGT
BsrFI 141 GCCGGC [GA]^CCGG[CT]
BstF5I 753 GGATG GGATG(2/0)
BstYI 158 GGATCT [GA]^GATC[CT]
CfoI 293 GCGC GCG^C
Cfr10I 141 GCCGGC [GA]^CCGG[CT]
DsaI 820 CCACGG C^C[GA][CT]GG
EarI 405 GAAGAG CTCTTC(1/4)
EcoNI 850 CCTTGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 211 GAATTC G^AATTC
EcoRV 901 GATATC GAT^ATC
FauI 81 GCGGG CCCGC(4/6)
FokI 753 GGATG GGATG(9/13)
HaeII 292 GGCGCC [GA]GCGC^[CT]
HhaI 293 GCGC GCG^C
HinP1I 293 GCGC G^CGC
KasI 292 GGCGCC G^GCGCC
Ksp632I 405 GAAGAG CTCTTC(1/4)
MfeI 382 CAATTG C^AATTG
MflI 158 GGATCT [GA]^GATC[CT]
MluNI 386 TGGCCA TGG^CCA
MscI 386 TGGCCA TGG^CCA
MslI 863 CACCAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 382 CAATTG C^AATTG
NaeI 141 GCCGGC GCC^GGC
NarI 292 GGCGCC GG^CGCC
NgoMIV 141 GCCGGC G^CCGGC
NlaIII 528 CATG CATG^
NspI 527 ACATGT [GA]CATG^[CT]
PpuMI 195 GGGTCCC [GA]G^G[AT]CC[CT]
PvuII 742 CAGCTG CAG^CTG
RsaI 423 GTAC GT^AC
SacI 455 GAGCTC GAGCT^C
SalI 797 GTCGAC G^TCGAC
SbfI 233 CCTGCAGG CCTGCA^GG
SexAI 97 ACCTGGT A^CC[AT]GGT
SfoI 292 GGCGCC GGC^GCC
SmaI 300 CCCGGG CCC^GGG
Sse8387I 233 CCTGCAGG CCTGCA^GG
XcmI 111 CCAGGCGGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 158 GGATCT [GA]^GATC[CT]
XmaCI 300 CCCGGG C^CCGGG
XmaI 300 CCCGGG C^CCGGG
XmnI 678 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcsI 211 GAATTC [GA]^AATT[CT]
679 AAATTC
AcyI 278 GACGCC G[GA]^CG[CT]C
292 GGCGCC
ApoI 211 GAATTC [GA]^AATT[CT]
679 AAATTC
AvaI 300 CCCGGG C^[CT]CG[GA]G
357 CTCGGG
BfaI 254 CTAG C^TAG
418 CTAG
BlpI 474 GCTTAGC GC^T[ACGT]AGC
513 GCTAAGC
BpmI 739 CTCCAG CTGGAG(16/14)
673 CTGGAG
Bpu1102I 474 GCTTAGC GC^T[ACGT]AGC
513 GCTAAGC
BsaHI 278 GACGCC G[GA]^CG[CT]C
292 GGCGCC
BseRI 945 CTCCTC GAGGAG(10/8)
973 CTCCTC
BsmAI 636 GAGAC GTCTC(1/5)
920 GTCTC
BsmBI 919 CGTCTC CGTCTC(1/5)
636 GAGACG
BsoBI 300 CCCGGG C^[CT]CG[GA]G
357 CTCGGG
BsrDI 312 CATTGC GCAATG(2/0)
726 CATTGC
BstXI 723 CCACATTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
959 CCACCACTCTGG
CelII 474 GCTTAGC GC^T[ACGT]AGC
513 GCTAAGC
EcoT14I 389 CCAAGG C^C[AT][AT]GG
417 CCTAGG
HgaI 278 GACGC GACGC(5/10)
518 GCGTC
Hin1I 278 GACGCC G[GA]^CG[CT]C
292 GGCGCC
HincII 85 GTTGAC GT[CT]^[GA]AC
797 GTCGAC
HindII 85 GTTGAC GT[CT]^[GA]AC
797 GTCGAC
HinfI 186 GATTC G^A[ACGT]TC
659 GAATC
HphI 129 GGTGA GGTGA(8/7)
876 TCACC
MaeI 254 CTAG C^TAG
418 CTAG
MaeII 176 ACGT A^CGT
918 ACGT
MboII 405 GAAGA GAAGA(8/7)
683 TCTTC
MspA1I 688 CAGCGG C[AC]G^C[GT]G
742 CAGCTG
PflMI 40 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
524 CCAACATGTGG
SfaNI 72 GATGC GCATC(5/9)
811 GATGC
StyI 389 CCAAGG C^C[AT][AT]GG
417 CCTAGG
TfiI 186 GATTC G^A[AT]TC
659 GAATC
Van91I 40 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
524 CCAACATGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AflIII AluI AlwI AspHI
BanI BanII BbvI BcnI BmyI
BsaJI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstNI Cac8I
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII Fnu4HI HaeIII HapII
HpaII ItaI MboI MnlI MspI
MvaI MwoI NciI NdeII NlaIV
PstI Sau3AI Sau96I ScrFI SfcI
TaqI TseI Tsp509I TspRI TthHB8I