##################################
# NO CUT ENZYMES (  195 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanII       BbeI        BbrPI       BbsI        BbvCI       
BbvI        BcgI        BciVI       BclI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
Cfr13I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        DsaI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FbaI        FseI        FspI        
HaeII       HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        KasI        Ksp632I     
KspI        MaeI        MaeII       MamI        MboII       
MfeI        MluI        MluNI       MroI        MscI        
MseI        MslI        MspA1I      MunI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NlaIII      NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        Sau96I      SbfI        
ScaI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StyI        SwaI        
Tru9I       TseI        Tth111I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

Acc65I        278  GGTACC               G^GTACC
AccII         262  CGCG                 CG^CG
AfaI          279  GTAC                 GT^AC
Asp718I       278  GGTACC               G^GTACC
BcnI          275  CCCGG                CC^[GC]GG
BsaI          119  GAGACC               GGTCTC(1/5)
BsmFI         349  GTCCC                GGGAC(10/14)
BstF5I        189  CATCC                GGATG(2/0)
BstUI         262  CGCG                 CG^CG
BstYI         308  AGATCC               [GA]^GATC[CT]
Cac8I          82  GCTCGC               GC[ACGT]^[ACGT]GC
DdeI          137  CTGAG                C^T[ACGT]AG
EaeI          258  TGGCCG               [CT]^GGCC[GA]
FauI          323  CCCGC                CCCGC(4/6)
Fnu4HI        260  GCCGC                GC^[ACGT]GC
FokI          189  CATCC                GGATG(9/13)
HapII         276  CCGG                 C^CGG
HgaI          347  GCGTC                GACGC(5/10)
HinfI         143  GAATC                G^A[ACGT]TC
HpaII         276  CCGG                 C^CGG
ItaI          260  GCCGC                GC^[ACGT]GC
KpnI          278  GGTACC               GGTAC^C
MaeIII        286  GTGAC                ^GT[ACGT]AC
MflI          308  AGATCC               [GA]^GATC[CT]
MspI          276  CCGG                 C^CGG
MvnI          262  CGCG                 CG^CG
NciI          275  CCCGG                CC^[GC]GG
RsaI          279  GTAC                 GT^AC
SexAI         281  ACCTGGT              A^CC[AT]GGT
SfaNI         165  GCATC                GCATC(5/9)
StuI           36  AGGCCT               AGG^CCT
TaqI           77  TCGA                 T^CGA
TfiI          143  GAATC                G^A[AT]TC
Tsp45I        286  GTGAC                ^GT[GC]AC
Tsp509I         9  AATT                 ^AATT
TthHB8I        77  TCGA                 T^CGA
XhoII         308  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AciI          261  CCGC                 CCGC(-3/-1)
              324  CCGC                
AlwI          309  GATCC                GGATC(4/5)
              237  GGATC               
BanI          271  GGCACC               G^G[CT][GA]CC
              278  GGTACC              
BpmI           32  CTGGAG               CTGGAG(16/14)
              196  CTGGAG              
BsaJI         114  CCTGGG               C^C[ACGT][ACGT]GG
              232  CCTGGG              
BsiYI          20  CCAGTGCGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              276  CCGGTACCTGG         
BslI           20  CCAGTGCGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              276  CCGGTACCTGG         
BsmAI         119  GAGAC                GTCTC(1/5)
              265  GAGAC               
HaeIII         37  GGCC                 GG^CC
              259  GGCC                
HphI          285  GGTGA                GGTGA(8/7)
              341  TCACC               
MwoI           54  GCTGATGGAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              263  GCGAGACTGGC         
NlaIV         271  GGCACC               GG[ACGT]^[ACGT]CC
              278  GGTACC              
SfcI           64  CTACAG               C^T[GA][CT]AG
               87  CTACAG              


#########################################
# 3 OR MORE CUT ENZYMES (   13 enzymes) #
#########################################

AluI        BsrI        BssKI       BstNI       DpnII       
EcoRII      MboI        MnlI        MvaI        NdeII       
Sau3AI      ScrFI       TspRI