Restriction map for
pF1KB0928
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI ApaI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BamHI BanI BbeI
BciVI BclI BfaI BfrI BglII
BlnI BlpI BpmI Bpu1102I BsaBI
BsaHI BseAI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstNI BstPI BstXI BstYI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco52I
EcoNI EcoO65I EcoRII EcoRV EcoT22I
FbaI FseI FspI Hin1I HincII
HindII HpaI KasI KpnI KspI
MaeI MamI MfeI MflI MroI
MunI MvaI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PleI PmeI
PpuMI PshAI PshBI Psp1406I RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Van91I XbaI XcmI XhoI
XhoII
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# ONE CUT ENZYMES ( 54 enzymes) #
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AcsI 1023 GAATTC [GA]^AATT[CT]
AflIII 638 ACGCGT A^C[GA][CT]GT
Alw44I 118 GTGCAC G^TGCAC
AocI 836 CCTCAGG CC^T[ACGT]AGG
ApaLI 118 GTGCAC G^TGCAC
ApoI 1023 GAATTC [GA]^AATT[CT]
Asp700I 6 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 118 GTGCAC G[AT]GC[AT]^C
AspI 741 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 779 TGGCCA TGG^CCA
BanII 924 GAGCCC G[GA]GC[CT]^C
BbrPI 424 CACGTG CAC^GTG
BbsI 127 GAAGAC GAAGAC(2/6)
BbvCI 142 GCTGAGG CCTCAGC(-5/-2)
BcgI 45 GCAGGAGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1066 GCCGATCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 127 GAAGAC GAAGAC(2/6)
BsaAI 424 CACGTG [CT]AC^GT[GA]
BsaI 470 GAGACC GGTCTC(1/5)
BsaWI 306 TCCGGT [AT]^CCGG[AT]
BseRI 295 GAGGAG GAGGAG(10/8)
BsiEI 1068 CGATCG CG[GA][CT]^CG
BsiHKAI 118 GTGCAC G[AT]GC[AT]^C
BsmAI 470 GAGAC GTCTC(1/5)
BsmI 419 GAATGC GAATGC(1/-1)
BsrFI 443 GCCGGC [GA]^CCGG[CT]
Bsu36I 836 CCTCAGG CC^T[ACGT]AGG
Cfr10I 443 GCCGGC [GA]^CCGG[CT]
DraII 386 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 776 CCATGG C^C[GA][CT]GG
EaeI 779 TGGCCA [CT]^GGCC[GA]
EarI 61 CTCTTC CTCTTC(1/4)
Eco81I 836 CCTCAGG CC^T[ACGT]AGG
EcoO109I 386 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 1023 GAATTC G^AATTC
EcoT14I 776 CCATGG C^C[AT][AT]GG
HgaI 933 GCGTC GACGC(5/10)
Ksp632I 61 CTCTTC CTCTTC(1/4)
MluI 638 ACGCGT A^CGCGT
MluNI 779 TGGCCA TGG^CCA
MscI 779 TGGCCA TGG^CCA
MseI 101 TTAA T^TAA
NaeI 443 GCCGGC GCC^GGC
NcoI 776 CCATGG C^CATGG
NgoMIV 443 GCCGGC G^CCGGC
NspI 240 GCATGT [GA]CATG^[CT]
PflFI 741 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 424 CACGTG CAC^GTG
PmlI 424 CACGTG CAC^GTG
PvuI 1068 CGATCG CGAT^CG
StyI 776 CCATGG C^C[AT][AT]GG
Tru9I 101 TTAA T^TAA
Tth111I 741 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 6 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwI 808 GATCC GGATC(4/5)
918 GGATC
Aor51HI 648 AGCGCT AGC^GCT
668 AGCGCT
AvaI 29 CCCGGG C^[CT]CG[GA]G
460 CCCGGG
BsiWI 592 CGTACG C^GTACG
641 CGTACG
BsoBI 29 CCCGGG C^[CT]CG[GA]G
460 CCCGGG
BstAPI 361 GCAGAGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
871 GCAGCCGCTGC
Eco47III 648 AGCGCT AGC^GCT
668 AGCGCT
HaeII 648 AGCGCT [GA]GCGC^[CT]
668 AGCGCT
HindIII 175 AAGCTT A^AGCTT
1004 AAGCTT
HinfI 51 GATTC G^A[ACGT]TC
619 GATTC
MaeII 197 ACGT A^CGT
425 ACGT
MboII 127 GAAGA GAAGA(8/7)
62 TCTTC
MslI 192 CATCAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
985 CATGAGAGTG
NlaIV 386 GGGGCC GG[ACGT]^[ACGT]CC
923 GGAGCC
SmaI 29 CCCGGG CCC^GGG
460 CCCGGG
TfiI 51 GATTC G^A[AT]TC
619 GATTC
XmaCI 29 CCCGGG C^CCGGG
460 CCCGGG
XmaI 29 CCCGGG C^CCGGG
460 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AvaII BbvI BcnI BmyI BsaJI
BsgI BsiYI BslI BsmFI Bsp1286I
BspMI BsrBI BsrI BssKI BstF5I
BstUI Cac8I CfoI Cfr13I DdeI
DpnII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MaeIII MboI MnlI MspA1I
MspI MvnI MwoI NciI NdeII
NlaIII PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I