Restriction map for
pF1KE0690
##################################
# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI AlwNI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BalI BbrPI
BbsI BcgI BciVI BclI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BseAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I CelII ClaI CpoI DraI
DraIII Eam1105I EarI Eco47III EcoNI
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI Ksp632I MamI MfeI MluI
MluNI MroI MscI MslI MunI
NdeI NheI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SapI
SbfI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TaqI TthHB8I Van91I XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AcsI 340 GAATTC [GA]^AATT[CT]
AflIII 1192 ACATGT A^C[GA][CT]GT
Alw44I 670 GTGCAC G^TGCAC
AocI 172 CCTGAGG CC^T[ACGT]AGG
ApaLI 670 GTGCAC G^TGCAC
ApoI 340 GAATTC [GA]^AATT[CT]
AspI 1183 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BfaI 1042 CTAG C^TAG
BsaWI 123 TCCGGT [AT]^CCGG[AT]
BspLU11I 1192 ACATGT A^CATGT
BspMI 464 ACCTGC ACCTGC(4/8)
Bsu36I 172 CCTGAGG CC^T[ACGT]AGG
DrdI 1086 GACTTCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 172 CCTGAGG CC^T[ACGT]AGG
EcoRI 340 GAATTC G^AATTC
KspI 70 CCGCGG CCGC^GG
MaeI 1042 CTAG C^TAG
MboII 294 GAAGA GAAGA(8/7)
MseI 1001 TTAA T^TAA
NaeI 96 GCCGGC GCC^GGC
NcoI 116 CCATGG C^CATGG
NgoMIV 96 GCCGGC G^CCGGC
NotI 195 GCGGCCGC GC^GGCCGC
NspI 1192 ACATGT [GA]CATG^[CT]
PflFI 1183 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 406 CTGCAG CTGCA^G
PvuII 628 CAGCTG CAG^CTG
SacII 70 CCGCGG CCGC^GG
ScaI 827 AGTACT AGT^ACT
SexAI 1184 ACCAGGT A^CC[AT]GGT
SfaNI 1156 GATGC GCATC(5/9)
SmlI 361 CTCAAG C^T[CT][GA]AG
StuI 149 AGGCCT AGG^CCT
Tru9I 1001 TTAA T^TAA
Tsp509I 341 AATT ^AATT
Tth111I 1183 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AfaI 32 GTAC GT^AC
828 GTAC
ApaI 275 GGGCCC GGGCC^C
347 GGGCCC
BamHI 120 GGATCC G^GATCC
500 GGATCC
BglI 151 GCCTTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
277 GCCCGGGTGGC
BseRI 394 GAGGAG GAGGAG(10/8)
517 GAGGAG
BsgI 652 CTGCAC GTGCAG(16/14)
385 GTGCAG
BsiEI 131 CGGCCG CG[GA][CT]^CG
196 CGGCCG
BsrBI 46 GAGCGG CCGCTC(-3/-3)
709 GAGCGG
BsrFI 96 GCCGGC [GA]^CCGG[CT]
1148 ACCGGC
BsrI 830 ACTGG ACTGG(1/-1)
64 CCAGT
Cfr10I 96 GCCGGC [GA]^CCGG[CT]
1148 ACCGGC
DsaI 70 CCGCGG C^C[GA][CT]GG
116 CCATGG
EagI 131 CGGCCG C^GGCCG
196 CGGCCG
EclXI 131 CGGCCG C^GGCCG
196 CGGCCG
Eco52I 131 CGGCCG C^GGCCG
196 CGGCCG
EcoT14I 116 CCATGG C^C[AT][AT]GG
899 CCAAGG
HgaI 307 GACGC GACGC(5/10)
401 GCGTC
HphI 1030 GGTGA GGTGA(8/7)
353 TCACC
MaeII 320 ACGT A^CGT
380 ACGT
PleI 964 GACTC GAGTC(4/5)
435 GAGTC
PpuMI 677 GGGACCT [GA]G^G[AT]CC[CT]
991 GGGACCT
RsaI 32 GTAC GT^AC
828 GTAC
SacI 450 GAGCTC GAGCT^C
982 GAGCTC
SfcI 406 CTGCAG C^T[GA][CT]AG
625 CTACAG
StyI 116 CCATGG C^C[AT][AT]GG
899 CCAAGG
TfiI 570 GAATC G^A[AT]TC
950 GAATC
Tsp45I 437 GTCAC ^GT[GC]AC
1189 GTCAC
TspRI 65 CAGTG CA[GC]TG[ACGT][ACGT]^
257 CAGTG
XcmI 116 CCATGGATCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
725 CCATCCTCAAAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AspHI AvaI AvaII BanI BanII
BbeI BbvCI BbvI BcnI BmyI
BsaHI BsaI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrDI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII ItaI KasI MaeIII MboI
MflI MnlI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfoI SmaI TseI XhoII XmaCI
XmaI