Restriction map for
pF1KB0947
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# NO CUT ENZYMES ( 186 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BanI BanII BbeI BbrPI BbvCI
BbvI BcgI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI BmyI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI BstBI BstEII
BstPI BstUI BstXI Bsu36I CelII
CfoI ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI Fnu4HI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI ItaI KasI
KpnI KspI MaeI MaeII MaeIII
MamI MfeI MluI MluNI MroI
MscI MslI MspA1I MunI MvnI
NarI NciI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TseI Tsp45I
Tth111I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 285 GTATAC GT^[AC][GT]AC
AlwNI 521 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 438 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 27 GGATCC G^GATCC
BbsI 157 GTCTTC GAAGAC(2/6)
BpmI 317 CTGGAG CTGGAG(16/14)
BpuAI 157 GTCTTC GAAGAC(2/6)
BsaJI 267 CCAAGG C^C[ACGT][ACGT]GG
BspHI 503 TCATGA T^CATGA
BsrFI 3 GCCGGC [GA]^CCGG[CT]
BsrI 418 ACTGG ACTGG(1/-1)
Bst1107I 285 GTATAC GTA^TAC
BstAPI 343 GCAATTTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 126 GGATG GGATG(2/0)
BstYI 27 GGATCC [GA]^GATC[CT]
BstZ17I 285 GTATAC GTA^TAC
Cac8I 3 GCCGGC GC[ACGT]^[ACGT]GC
Cfr10I 3 GCCGGC [GA]^CCGG[CT]
DpnII 28 GATC ^GATC
EcoT14I 267 CCAAGG C^C[AT][AT]GG
FokI 126 GGATG GGATG(9/13)
HaeIII 150 GGCC GG^CC
HapII 4 CCGG C^CGG
HpaII 4 CCGG C^CGG
HphI 271 GGTGA GGTGA(8/7)
MboI 28 GATC ^GATC
MflI 27 GGATCC [GA]^GATC[CT]
MnlI 451 CCTC CCTC(7/6)
MspI 4 CCGG C^CGG
NaeI 3 GCCGGC GCC^GGC
NdeII 28 GATC ^GATC
NgoMIV 3 GCCGGC G^CCGGC
NlaIV 27 GGATCC GG[ACGT]^[ACGT]CC
PflMI 467 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 513 GAGTC GAGTC(4/5)
RcaI 503 TCATGA T^CATGA
Sau3AI 28 GATC ^GATC
StyI 267 CCAAGG C^C[AT][AT]GG
TaqI 446 TCGA T^CGA
TfiI 296 GAATC G^A[AT]TC
TspRI 59 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 446 TCGA T^CGA
Van91I 467 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 27 GGATCC [GA]^GATC[CT]
XmnI 438 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 11 enzymes) #
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AluI 189 AGCT AG^CT
404 AGCT
AlwI 28 GATCC GGATC(4/5)
27 GGATC
BsiYI 310 CCCATTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
467 CCAGCAAGTGG
BslI 310 CCCATTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
467 CCAGCAAGTGG
BspMI 90 ACCTGC ACCTGC(4/8)
357 ACCTGC
EarI 15 CTCTTC CTCTTC(1/4)
492 GAAGAG
HinfI 296 GAATC G^A[ACGT]TC
513 GAGTC
Ksp632I 15 CTCTTC CTCTTC(1/4)
492 GAAGAG
MseI 179 TTAA T^TAA
338 TTAA
MwoI 343 GCAATTTATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
352 GCCAAACCTGC
Tru9I 179 TTAA T^TAA
338 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AvaII BsmAI BssKI BstNI Cfr13I
DdeI DraII EcoO109I EcoRII MboII
MvaI NlaIII PpuMI Sau96I ScrFI
Tsp509I