Restriction map for
pF1KB0959
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AlwI Aor51HI ApaI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AvrII BalI BamHI
BanII BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaI
BseAI BsiEI BsiWI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI Bst1107I BstBI
BstXI BstYI BstZ17I CelII ClaI
CpoI DpnII DraI DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KpnI KspI MaeI MamI
MboI MfeI MflI MluI MluNI
MroI MscI MseI MslI MunI
NcoI NdeI NdeII NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI Sau3AI
ScaI SexAI SfiI SfuI SmaI
SmlI SnaBI SpeI SphI SspBI
SspI StyI SwaI TaqI TfiI
Tru9I Tsp509I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Alw44I 346 GTGCAC G^TGCAC
AocI 261 CCTCAGG CC^T[ACGT]AGG
ApaLI 346 GTGCAC G^TGCAC
BbeI 225 GGCGCC GGCGC^C
BbrPI 445 CACGTG CAC^GTG
BcnI 23 CCGGG CC^[GC]GG
BpmI 466 CTGGAG CTGGAG(16/14)
BsaAI 445 CACGTG [CT]AC^GT[GA]
BsaWI 335 ACCGGA [AT]^CCGG[AT]
BseRI 99 CTCCTC GAGGAG(10/8)
BsgI 232 GTGCAG GTGCAG(16/14)
BsmBI 511 GAGACG CGTCTC(1/5)
BspMI 299 ACCTGC ACCTGC(4/8)
BssHII 328 GCGCGC G^CGCGC
BssSI 38 CACGAG CACGAG(-5/-1)
BstAPI 426 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 354 GGTGACC G^GT[ACGT]ACC
BstF5I 548 GGATG GGATG(2/0)
BstPI 354 GGTGACC G^GT[ACGT]ACC
Bsu36I 261 CCTCAGG CC^T[ACGT]AGG
DraIII 349 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 441 CGGCCA [CT]^GGCC[GA]
EarI 82 CTCTTC CTCTTC(1/4)
Eco81I 261 CCTCAGG CC^T[ACGT]AGG
EcoO65I 354 GGTGACC G^GT[ACGT]ACC
FokI 548 GGATG GGATG(9/13)
HaeII 225 GGCGCC [GA]GCGC^[CT]
KasI 225 GGCGCC G^GCGCC
Ksp632I 82 CTCTTC CTCTTC(1/4)
MaeIII 355 GTGAC ^GT[ACGT]AC
MboII 83 TCTTC GAAGA(8/7)
NaeI 439 GCCGGC GCC^GGC
NarI 225 GGCGCC GG^CGCC
NciI 23 CCGGG CC^[GC]GG
NgoMIV 439 GCCGGC G^CCGGC
NlaIII 275 CATG CATG^
NspI 274 ACATGC [GA]CATG^[CT]
PmaCI 445 CACGTG CAC^GTG
PmlI 445 CACGTG CAC^GTG
PvuII 556 CAGCTG CAG^CTG
SbfI 456 CCTGCAGG CCTGCA^GG
SfaNI 549 GATGC GCATC(5/9)
SfoI 225 GGCGCC GGC^GCC
SgrAI 227 CGCCGGTG C[GA]^CCGG[CT]G
Sse8387I 456 CCTGCAGG CCTGCA^GG
StuI 362 AGGCCT AGG^CCT
Tsp45I 355 GTGAC ^GT[GC]AC
TspRI 344 CAGTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 225 GGCGCC G[GA]^CG[CT]C
513 GACGCC
AfaI 297 GTAC GT^AC
544 GTAC
AspHI 339 GAGCAC G[AT]GC[AT]^C
346 GTGCAC
AvaII 26 GGTCC G^G[AT]CC
267 GGTCC
AviII 302 TGCGCA TGC^GCA
449 TGCGCA
BanI 19 GGCACC G^G[CT][GA]CC
225 GGCGCC
BglI 278 GCCGCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
417 GCCTCGCAGGC
BsaHI 225 GGCGCC G[GA]^CG[CT]C
513 GACGCC
BsiHKAI 339 GAGCAC G[AT]GC[AT]^C
346 GTGCAC
BsmAI 511 GAGAC GTCTC(1/5)
126 GTCTC
BsrFI 228 GCCGGT [GA]^CCGG[CT]
439 GCCGGC
BsrI 171 CCAGT ACTGG(1/-1)
294 CCAGT
Cfr10I 228 GCCGGT [GA]^CCGG[CT]
439 GCCGGC
DdeI 241 CTGAG C^T[ACGT]AG
262 CTCAG
FspI 302 TGCGCA TGC^GCA
449 TGCGCA
Hin1I 225 GGCGCC G[GA]^CG[CT]C
513 GACGCC
HinfI 76 GACTC G^A[ACGT]TC
145 GAGTC
HphI 354 GGTGA GGTGA(8/7)
31 TCACC
MaeII 58 ACGT A^CGT
446 ACGT
PleI 76 GACTC GAGTC(4/5)
145 GAGTC
PpuMI 25 GGGTCCT [GA]G^G[AT]CC[CT]
266 GGGTCCC
PstI 457 CTGCAG CTGCA^G
499 CTGCAG
RsaI 297 GTAC GT^AC
544 GTAC
SfcI 457 CTGCAG C^T[GA][CT]AG
499 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AccII AciI AluI AlwNI BbvI
BmyI BsaJI BsiYI BslI BsmFI
Bsp1286I BssKI BstNI BstUI Cac8I
CfoI Cfr13I DraII EcoO109I EcoRII
FauI Fnu4HI HaeIII HapII HgaI
HhaI HinP1I HpaII ItaI MnlI
MspA1I MspI MvaI MvnI MwoI
NlaIV Sau96I ScrFI TseI