Restriction map for
pF1KE0728
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Alw44I AocI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BbsI
BbvCI BclI BfrI BglII BlnI
BpuAI BsaBI BseRI BsiWI BspDI
BspHI BspLU11I BsrBI BssHII Bst1107I
BstEII BstPI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DraII DraIII
DrdI Eam1105I EarI Eco81I EcoNI
EcoO109I EcoO65I EcoRV EcoT14I EcoT22I
FbaI FseI FspI HindIII HpaI
HphI KpnI Ksp632I MamI MfeI
MluI MunI NaeI NcoI NdeI
NgoMIV NheI NruI NsiI PacI
PaeR7I PflFI PflMI PinAI PleI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfiI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI StyI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccI 712 GTCTAC GT^[AC][GT]AC
AccIII 450 TCCGGA T^CCGGA
AcsI 724 GAATTC [GA]^AATT[CT]
AflIII 639 ACGTGT A^C[GA][CT]GT
AluI 60 AGCT AG^CT
AlwNI 101 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 301 AGCGCT AGC^GCT
ApoI 724 GAATTC [GA]^AATT[CT]
AspHI 238 GAGCAC G[AT]GC[AT]^C
AvaI 235 CCCGAG C^[CT]CG[GA]G
BalI 357 TGGCCA TGG^CCA
BamHI 447 GGATCC G^GATCC
BanII 285 GAGCCC G[GA]GC[CT]^C
BbeI 918 GGCGCC GGCGC^C
BbrPI 638 CACGTG CAC^GTG
BglI 405 GCCCAAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 124 GCTCAGC GC^T[ACGT]AGC
BpmI 1103 CTCCAG CTGGAG(16/14)
Bpu1102I 124 GCTCAGC GC^T[ACGT]AGC
BsaAI 638 CACGTG [CT]AC^GT[GA]
BsaI 1189 GGTCTC GGTCTC(1/5)
BsaWI 450 TCCGGA [AT]^CCGG[AT]
BseAI 450 TCCGGA T^CCGGA
BsgI 707 GTGCAG GTGCAG(16/14)
BsiHKAI 238 GAGCAC G[AT]GC[AT]^C
BsmFI 719 GTCCC GGGAC(10/14)
BsmI 1134 GAATGC GAATGC(1/-1)
BsoBI 235 CCCGAG C^[CT]CG[GA]G
BspEI 450 TCCGGA T^CCGGA
BspMI 872 ACCTGC ACCTGC(4/8)
BsrFI 54 ACCGGC [GA]^CCGG[CT]
BssSI 297 CACGAG CACGAG(-5/-1)
BstAPI 100 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 547 TTCGAA TT^CGAA
CelII 124 GCTCAGC GC^T[ACGT]AGC
Cfr10I 54 ACCGGC [GA]^CCGG[CT]
EagI 851 CGGCCG C^GGCCG
EclXI 851 CGGCCG C^GGCCG
Eco47III 301 AGCGCT AGC^GCT
Eco52I 851 CGGCCG C^GGCCG
EcoRI 724 GAATTC G^AATTC
HincII 664 GTCAAC GT[CT]^[GA]AC
HindII 664 GTCAAC GT[CT]^[GA]AC
KasI 918 GGCGCC G^GCGCC
MaeII 639 ACGT A^CGT
MluNI 357 TGGCCA TGG^CCA
MroI 450 TCCGGA T^CCGGA
MscI 357 TGGCCA TGG^CCA
NarI 918 GGCGCC GG^CGCC
NotI 850 GCGGCCGC GC^GGCCGC
NspI 417 GCATGT [GA]CATG^[CT]
NspV 547 TTCGAA TT^CGAA
PmaCI 638 CACGTG CAC^GTG
PmlI 638 CACGTG CAC^GTG
PstI 988 CTGCAG CTGCA^G
SfoI 918 GGCGCC GGC^GCC
SfuI 547 TTCGAA TT^CGAA
SmlI 11 CTCAAG C^T[CT][GA]AG
Tsp45I 1052 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 6 GGCGTC G[GA]^CG[CT]C
918 GGCGCC
AvaII 453 GGACC G^G[AT]CC
741 GGTCC
BcgI 549 CGAAAATAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
723 CGAATTCTATGC
BfaI 1166 CTAG C^TAG
1214 CTAG
BsaHI 6 GGCGTC G[GA]^CG[CT]C
918 GGCGCC
BsiEI 851 CGGCCG CG[GA][CT]^CG
885 CGGTCG
BsmBI 8 CGTCTC CGTCTC(1/5)
312 CGTCTC
Bsp1407I 779 TGTACA T^GTACA
896 TGTACA
BsrDI 1205 CATTGC GCAATG(2/0)
814 GCAATG
BsrGI 779 TGTACA T^GTACA
896 TGTACA
BstYI 447 GGATCC [GA]^GATC[CT]
769 GGATCT
EaeI 357 TGGCCA [CT]^GGCC[GA]
851 CGGCCG
HaeII 301 AGCGCT [GA]GCGC^[CT]
918 GGCGCC
Hin1I 6 GGCGTC G[GA]^CG[CT]C
918 GGCGCC
HinfI 81 GATTC G^A[ACGT]TC
693 GAATC
MaeI 1166 CTAG C^TAG
1214 CTAG
MboII 772 TCTTC GAAGA(8/7)
1201 TCTTC
MflI 447 GGATCC [GA]^GATC[CT]
769 GGATCT
MseI 247 TTAA T^TAA
509 TTAA
MslI 28 CATCCTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1174 CATGCTGATG
SspBI 779 TGTACA T^GTACA
896 TGTACA
TaqI 548 TCGA T^CGA
617 TCGA
TfiI 81 GATTC G^A[AT]TC
693 GAATC
Tru9I 247 TTAA T^TAA
509 TTAA
TthHB8I 548 TCGA T^CGA
617 TCGA
XhoII 447 GGATCC [GA]^GATC[CT]
769 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AfaI AlwI BanI
BbvI BciVI BcnI BmyI BsaJI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HpaII
ItaI KspI MaeIII MboI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI TseI Tsp509I TspRI