Restriction map for
pF1KE0986
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AhdI Alw44I AocI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvrII BalI BbeI BbsI BcgI
BciVI BglII BlnI BpuAI BsaHI
BsaI BseAI BsiWI BsmAI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII BssSI Bst1107I BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI Eam1105I EarI Eco81I EcoRI
EcoRV FseI HgaI Hin1I HindIII
KasI KpnI Ksp632I KspI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NgoMIV NheI NotI
NruI PacI PaeR7I PflFI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SnaBI
SpeI Sse8387I SspBI SwaI Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 1135 GTCTAC GT^[AC][GT]AC
AfaI 1143 GTAC GT^AC
AflII 1045 CTTAAG C^TTAAG
AflIII 1139 ACACGT A^C[GA][CT]GT
AgeI 1669 ACCGGT A^CCGGT
AlwNI 1327 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 770 AGCGCT AGC^GCT
AviII 1565 TGCGCA TGC^GCA
BamHI 1554 GGATCC G^GATCC
BbvCI 1602 GCTGAGG CCTCAGC(-5/-2)
BclI 225 TGATCA T^GATCA
BfaI 1709 CTAG C^TAG
BfrI 1045 CTTAAG C^TTAAG
BglI 366 GCCAGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 798 GATCCGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 386 GCATTC GAATGC(1/-1)
BsrDI 475 GCAATG GCAATG(2/0)
BstAPI 483 GCAATACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 508 TTCGAA TT^CGAA
BstEII 1252 GGTTACC G^GT[ACGT]ACC
BstPI 1252 GGTTACC G^GT[ACGT]ACC
BstXI 192 CCACACGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 425 CCATGG C^C[GA][CT]GG
Eco47III 770 AGCGCT AGC^GCT
EcoNI 1598 CCTGGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1252 GGTTACC G^GT[ACGT]ACC
EcoT22I 1340 ATGCAT ATGCA^T
FbaI 225 TGATCA T^GATCA
FspI 1565 TGCGCA TGC^GCA
HincII 3 GTTAAC GT[CT]^[GA]AC
HindII 3 GTTAAC GT[CT]^[GA]AC
HpaI 3 GTTAAC GTT^AAC
MaeI 1709 CTAG C^TAG
MamI 798 GATCCGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 425 CCATGG C^CATGG
NsiI 1340 ATGCAT ATGCA^T
NspV 508 TTCGAA TT^CGAA
PinAI 1669 ACCGGT A^CCGGT
PleI 1404 GACTC GAGTC(4/5)
PpuMI 668 AGGTCCT [GA]G^G[AT]CC[CT]
RsaI 1143 GTAC GT^AC
SfuI 508 TTCGAA TT^CGAA
SgrAI 1668 CACCGGTG C[GA]^CCGG[CT]G
SmaI 90 CCCGGG CCC^GGG
SspI 1765 AATATT AAT^ATT
StuI 713 AGGCCT AGG^CCT
XcmI 425 CCATGGAAGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 90 CCCGGG C^CCGGG
XmaI 90 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccII 1154 CGCG CG^CG
1494 CGCG
ApaI 589 GGGCCC GGGCC^C
1464 GGGCCC
Asp700I 768 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
942 GAATAAATTC
AvaI 90 CCCGGG C^[CT]CG[GA]G
1051 CCCGAG
BbrPI 649 CACGTG CAC^GTG
739 CACGTG
BlpI 909 GCTGAGC GC^T[ACGT]AGC
1712 GCTCAGC
Bpu1102I 909 GCTGAGC GC^T[ACGT]AGC
1712 GCTCAGC
BsaWI 1470 ACCGGA [AT]^CCGG[AT]
1669 ACCGGT
BseRI 793 GAGGAG GAGGAG(10/8)
1605 GAGGAG
BsoBI 90 CCCGGG C^[CT]CG[GA]G
1051 CCCGAG
BstUI 1154 CGCG CG^CG
1494 CGCG
CelII 909 GCTGAGC GC^T[ACGT]AGC
1712 GCTCAGC
DraII 668 AGGTCCT [GA]G^G[ACGT]CC[CT]
1463 GGGGCCC
EaeI 20 CGGCCG [CT]^GGCC[GA]
48 CGGCCG
EagI 20 CGGCCG C^GGCCG
48 CGGCCG
EclXI 20 CGGCCG C^GGCCG
48 CGGCCG
Eco52I 20 CGGCCG C^GGCCG
48 CGGCCG
EcoO109I 668 AGGTCCT [GA]G^G[ACGT]CC[CT]
1463 GGGGCCC
FauI 31 CCCGC CCCGC(4/6)
135 CCCGC
MboII 939 GAAGA GAAGA(8/7)
1273 GAAGA
MvnI 1154 CGCG CG^CG
1494 CGCG
NdeI 626 CATATG CA^TATG
856 CATATG
PflMI 735 CCAGCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
855 CCATATGCTGG
PmaCI 649 CACGTG CAC^GTG
739 CACGTG
PmlI 649 CACGTG CAC^GTG
739 CACGTG
PstI 997 CTGCAG CTGCA^G
1559 CTGCAG
PvuII 376 CAGCTG CAG^CTG
1288 CAGCTG
SfcI 997 CTGCAG C^T[GA][CT]AG
1559 CTGCAG
SmlI 673 CTCAAG C^T[CT][GA]AG
1045 CTTAAG
SphI 1199 GCATGC GCATG^C
1316 GCATGC
TaqI 509 TCGA T^CGA
991 TCGA
TfiI 803 GAATC G^A[AT]TC
993 GATTC
Tsp45I 1040 GTGAC ^GT[GC]AC
1399 GTCAC
TthHB8I 509 TCGA T^CGA
991 TCGA
Van91I 735 CCAGCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
855 CCATATGCTGG
XmnI 768 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
942 GAATAAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AspHI AvaII BanI BanII BbvI
BcnI BmyI BpmI BsaAI BsaJI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
SacI Sau3AI Sau96I ScrFI SfaNI
StyI Tru9I TseI Tsp509I TspRI
XhoII