Restriction map for
pF1KB0409
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AgeI Alw44I AlwI ApaI
ApaLI ApoI AseI AsnI AspI
AvrII BalI BamHI BcgI BciVI
BfrI BglI BlnI BlpI Bpu1102I
BsaBI BsaWI BseAI BsmI BspDI
BspEI BspHI BsrBI BsrDI Bst1107I
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
EarI EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI HincII HindII HindIII
HpaI Ksp632I MamI MfeI MluNI
MroI MscI MseI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SapI SbfI SexAI
SfiI SfuI SnaBI SpeI SphI
Sse8387I SspI SwaI TfiI Tru9I
Tth111I XhoI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 1247 GGTACC G^GTACC
AhdI 547 GACGGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 506 AGCGCT AGC^GCT
AscI 176 GGCGCGCC GG^CGCGCC
Asp700I 291 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1247 GGTACC G^GTACC
AspEI 547 GACGGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1282 TGCGCA TGC^GCA
BbrPI 1242 CACGTG CAC^GTG
BbsI 130 GTCTTC GAAGAC(2/6)
BbvCI 816 CCTCAGC CCTCAGC(-5/-2)
BclI 725 TGATCA T^GATCA
BfaI 601 CTAG C^TAG
BglII 266 AGATCT A^GATCT
BpuAI 130 GTCTTC GAAGAC(2/6)
BsiEI 205 CGGCCG CG[GA][CT]^CG
BsiWI 878 CGTACG C^GTACG
BsmBI 424 GAGACG CGTCTC(1/5)
Bsp1407I 1307 TGTACA T^GTACA
BspLU11I 1157 ACATGT A^CATGT
BsrGI 1307 TGTACA T^GTACA
BssHII 177 GCGCGC G^CGCGC
BssSI 523 CACGAG CACGAG(-5/-1)
BstAPI 647 GCAAGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 266 AGATCT [GA]^GATC[CT]
EaeI 205 CGGCCG [CT]^GGCC[GA]
EagI 205 CGGCCG C^GGCCG
Eam1105I 547 GACGGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 205 CGGCCG C^GGCCG
Eco47III 506 AGCGCT AGC^GCT
Eco52I 205 CGGCCG C^GGCCG
FbaI 725 TGATCA T^GATCA
FspI 1282 TGCGCA TGC^GCA
HphI 693 GGTGA GGTGA(8/7)
KpnI 1247 GGTACC GGTAC^C
KspI 749 CCGCGG CCGC^GG
MaeI 601 CTAG C^TAG
MflI 266 AGATCT [GA]^GATC[CT]
MluI 185 ACGCGT A^CGCGT
NcoI 673 CCATGG C^CATGG
PmaCI 1242 CACGTG CAC^GTG
PmlI 1242 CACGTG CAC^GTG
SacII 749 CCGCGG CCGC^GG
ScaI 479 AGTACT AGT^ACT
SfaNI 1259 GCATC GCATC(5/9)
SmlI 333 CTCAAG C^T[CT][GA]AG
SspBI 1307 TGTACA T^GTACA
StuI 77 AGGCCT AGG^CCT
Tsp509I 636 AATT ^AATT
XbaI 600 TCTAGA T^CTAGA
XcmI 182 CCAACGCGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 266 AGATCT [GA]^GATC[CT]
XmnI 291 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflIII 185 ACGCGT A^C[GA][CT]GT
1157 ACATGT
AocI 15 CCTCAGG CC^T[ACGT]AGG
80 CCTCAGG
AvaI 260 CCCGGG C^[CT]CG[GA]G
885 CCCGGG
BbeI 170 GGCGCC GGCGC^C
1051 GGCGCC
BsaAI 876 CACGTA [CT]AC^GT[GA]
1242 CACGTG
BsoBI 260 CCCGGG C^[CT]CG[GA]G
885 CCCGGG
BspMI 678 GCAGGT ACCTGC(4/8)
858 GCAGGT
BstXI 734 CCATCTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1055 CCACGGCCCTGG
Bsu36I 15 CCTCAGG CC^T[ACGT]AGG
80 CCTCAGG
Eco81I 15 CCTCAGG CC^T[ACGT]AGG
80 CCTCAGG
EcoT14I 627 CCAAGG C^C[AT][AT]GG
673 CCATGG
FauI 65 GCGGG CCCGC(4/6)
849 GCGGG
KasI 170 GGCGCC G^GCGCC
1051 GGCGCC
MboII 131 TCTTC GAAGA(8/7)
269 TCTTC
MslI 430 CACAAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
735 CATCTGGGTG
NarI 170 GGCGCC GG^CGCC
1051 GGCGCC
PflMI 927 CCAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1035 CCAGCTGCTGG
PpuMI 680 AGGTCCT [GA]G^G[AT]CC[CT]
718 GGGACCC
SacI 701 GAGCTC GAGCT^C
1066 GAGCTC
SfoI 170 GGCGCC GGC^GCC
1051 GGCGCC
SgrAI 166 CACCGGCG C[GA]^CCGG[CT]G
172 CGCCGGCG
SmaI 260 CCCGGG CCC^GGG
885 CCCGGG
StyI 627 CCAAGG C^C[AT][AT]GG
673 CCATGG
Tsp45I 488 GTGAC ^GT[GC]AC
1119 GTCAC
Van91I 927 CCAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1035 CCAGCTGCTGG
XmaCI 260 CCCGGG C^CCGGG
885 CCCGGG
XmaI 260 CCCGGG C^CCGGG
885 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AspHI AvaII BanI BanII
BbvI BcnI BmyI BpmI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EcoO109I
EcoRII Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII ItaI MaeII MaeIII
MboI MnlI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfcI TaqI TseI TspRI
TthHB8I