Restriction map for
pF1KE0988
##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AgeI AhdI Aor51HI ApoI
AscI AseI AsnI AspEI AspI
AvrII BamHI BbeI BbrPI BcgI
BclI BfaI BfrI BglII BlnI
BsaBI BsaI BsaWI BseAI BsiWI
Bsp1407I BspDI BspEI BspLU11I BsrGI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DraI DrdI Eam1105I Eco47III
EcoRI EcoRV FbaI FseI HindIII
KasI MaeI MamI MfeI MroI
MunI NarI NdeI NheI NotI
NruI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RsrII SalI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tsp509I
Tth111I Van91I XbaI XcmI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 488 GGTACC G^GTACC
AflIII 505 ACGCGT A^C[GA][CT]GT
AocI 785 CCTCAGG CC^T[ACGT]AGG
ApaI 1816 GGGCCC GGGCC^C
Asp700I 984 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 488 GGTACC G^GTACC
AvaII 235 GGACC G^G[AT]CC
BciVI 871 GGATAC GTATCC(6/5)
BcnI 334 CCGGG CC^[GC]GG
BglI 784 GCCTCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1487 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1487 GCTCAGC GC^T[ACGT]AGC
BsaAI 376 TACGTG [CT]AC^GT[GA]
BspHI 1712 TCATGA T^CATGA
BstEII 1578 GGTCACC G^GT[ACGT]ACC
BstPI 1578 GGTCACC G^GT[ACGT]ACC
Bsu36I 785 CCTCAGG CC^T[ACGT]AGG
CelII 1487 GCTCAGC GC^T[ACGT]AGC
Eco81I 785 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1578 GGTCACC G^GT[ACGT]ACC
EcoT22I 1527 ATGCAT ATGCA^T
HaeII 185 AGCGCC [GA]GCGC^[CT]
HpaI 1408 GTTAAC GTT^AAC
HphI 1580 TCACC GGTGA(8/7)
KpnI 488 GGTACC GGTAC^C
MluI 505 ACGCGT A^CGCGT
MseI 1409 TTAA T^TAA
NaeI 214 GCCGGC GCC^GGC
NciI 334 CCGGG CC^[GC]GG
NgoMIV 214 GCCGGC G^CCGGC
NsiI 1527 ATGCAT ATGCA^T
PaeR7I 322 CTCGAG C^TCGAG
PleI 831 GACTC GAGTC(4/5)
RcaI 1712 TCATGA T^CATGA
ScaI 659 AGTACT AGT^ACT
SexAI 1328 ACCAGGT A^CC[AT]GGT
TfiI 848 GAATC G^A[AT]TC
Tru9I 1409 TTAA T^TAA
XhoI 322 CTCGAG C^TCGAG
XmnI 984 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AcyI 358 GACGCC G[GA]^CG[CT]C
1207 GGCGTC
Alw44I 151 GTGCAC G^TGCAC
1372 GTGCAC
AlwNI 112 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
788 CAGGGGCTG
ApaLI 151 GTGCAC G^TGCAC
1372 GTGCAC
AvaI 322 CTCGAG C^[CT]CG[GA]G
670 CCCGAG
AviII 293 TGCGCA TGC^GCA
1076 TGCGCA
BalI 1097 TGGCCA TGG^CCA
1799 TGGCCA
BbsI 615 GAAGAC GAAGAC(2/6)
340 GTCTTC
BbvCI 564 GCTGAGG CCTCAGC(-5/-2)
1840 GCTGAGG
BpuAI 615 GAAGAC GAAGAC(2/6)
340 GTCTTC
BsaHI 358 GACGCC G[GA]^CG[CT]C
1207 GGCGTC
BsgI 1264 CTGCAC GTGCAG(16/14)
1324 CTGCAC
BsiEI 310 CGGCCG CG[GA][CT]^CG
916 CGGCCG
BsmAI 356 GAGAC GTCTC(1/5)
843 GAGAC
BsmBI 356 GAGACG CGTCTC(1/5)
843 GAGACG
BsmFI 600 GTCCC GGGAC(10/14)
900 GTCCC
BsmI 1525 GAATGC GAATGC(1/-1)
665 GCATTC
BsoBI 322 CTCGAG C^[CT]CG[GA]G
670 CCCGAG
BsrI 1455 ACTGG ACTGG(1/-1)
111 CCAGT
BstXI 1448 CCAAAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1802 CCAGCAAGGTGG
BstYI 1295 AGATCC [GA]^GATC[CT]
1727 GGATCT
EagI 310 CGGCCG C^GGCCG
916 CGGCCG
EclXI 310 CGGCCG C^GGCCG
916 CGGCCG
Eco52I 310 CGGCCG C^GGCCG
916 CGGCCG
EcoNI 411 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
747 CCTGAAGGAGG
FspI 293 TGCGCA TGC^GCA
1076 TGCGCA
Hin1I 358 GACGCC G[GA]^CG[CT]C
1207 GGCGTC
HincII 828 GTTGAC GT[CT]^[GA]AC
1408 GTTAAC
HindII 828 GTTGAC GT[CT]^[GA]AC
1408 GTTAAC
HinfI 831 GACTC G^A[ACGT]TC
848 GAATC
MflI 1295 AGATCC [GA]^GATC[CT]
1727 GGATCT
MluNI 1097 TGGCCA TGG^CCA
1799 TGGCCA
MscI 1097 TGGCCA TGG^CCA
1799 TGGCCA
NspI 1073 GCATGC [GA]CATG^[CT]
1775 GCATGC
PvuII 700 CAGCTG CAG^CTG
1174 CAGCTG
SacI 244 GAGCTC GAGCT^C
970 GAGCTC
SapI 96 GCTCTTC GCTCTTC(1/4)
987 GCTCTTC
SfcI 954 CTACAG C^T[GA][CT]AG
1791 CTACAG
SphI 1073 GCATGC GCATG^C
1775 GCATGC
StuI 755 AGGCCT AGG^CCT
1592 AGGCCT
XhoII 1295 AGATCC [GA]^GATC[CT]
1727 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI BanI BanII BbvI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI Bsp1286I BspMI BsrBI BsrDI
BsrFI BssKI BssSI BstAPI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HpaII
ItaI Ksp632I KspI MaeII MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NcoI
NdeII NlaIII NlaIV RsaI SacII
Sau3AI Sau96I ScrFI SfaNI SmlI
StyI TaqI TseI Tsp45I TspRI
TthHB8I