Restriction map for
pF1KE0989
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# NO CUT ENZYMES ( 137 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Aor51HI ApaI AscI Asp718I
AspEI AspI AviII AvrII BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BclI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsiWI
BsmBI Bsp1407I BspEI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
CelII CfoI Cfr10I CpoI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoT14I FauI FbaI FseI FspI
HaeII HhaI Hin1I HinP1I HindIII
HpaI HphI KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI StyI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AcsI 632 AAATTT [GA]^AATT[CT]
AocI 1334 CCTCAGG CC^T[ACGT]AGG
ApoI 632 AAATTT [GA]^AATT[CT]
AseI 264 ATTAAT AT^TAAT
AsnI 264 ATTAAT AT^TAAT
Asp700I 766 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1116 CCCGAG C^[CT]CG[GA]G
BciVI 679 GGATAC GTATCC(6/5)
BcnI 77 CCGGG CC^[GC]GG
BfaI 1016 CTAG C^TAG
BglII 517 AGATCT A^GATCT
BpmI 1208 CTGGAG CTGGAG(16/14)
BsaAI 39 CACGTA [CT]AC^GT[GA]
BsgI 1663 GTGCAG GTGCAG(16/14)
BsiEI 814 CGACCG CG[GA][CT]^CG
BsmI 130 GAATGC GAATGC(1/-1)
BsoBI 1116 CCCGAG C^[CT]CG[GA]G
BspDI 982 ATCGAT AT^CGAT
BspHI 779 TCATGA T^CATGA
BspMI 1374 ACCTGC ACCTGC(4/8)
BsrDI 1589 GCAATG GCAATG(2/0)
Bsu36I 1334 CCTCAGG CC^T[ACGT]AGG
ClaI 982 ATCGAT AT^CGAT
Eco81I 1334 CCTCAGG CC^T[ACGT]AGG
EcoRV 1454 GATATC GAT^ATC
EcoT22I 132 ATGCAT ATGCA^T
HgaI 1061 GCGTC GACGC(5/10)
HincII 1410 GTCAAC GT[CT]^[GA]AC
HindII 1410 GTCAAC GT[CT]^[GA]AC
MaeI 1016 CTAG C^TAG
NciI 77 CCGGG CC^[GC]GG
NsiI 132 ATGCAT ATGCA^T
NspI 1133 ACATGC [GA]CATG^[CT]
PpuMI 1554 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 264 ATTAAT AT^TAAT
PstI 1030 CTGCAG CTGCA^G
RcaI 779 TCATGA T^CATGA
ScaI 1433 AGTACT AGT^ACT
SmlI 141 CTTGAG C^T[CT][GA]AG
XcmI 17 CCATGAGACAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 766 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AciI 1045 CCGC CCGC(-3/-1)
1099 CCGC
Alw44I 1805 GTGCAC G^TGCAC
1825 GTGCAC
AlwNI 1024 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
1051 CAGAACCTG
ApaLI 1805 GTGCAC G^TGCAC
1825 GTGCAC
AspHI 1805 GTGCAC G[AT]GC[AT]^C
1825 GTGCAC
AvaII 403 GGTCC G^G[AT]CC
1555 GGACC
BamHI 801 GGATCC G^GATCC
1682 GGATCC
BsiHKAI 1805 GTGCAC G[AT]GC[AT]^C
1825 GTGCAC
BsmAI 21 GAGAC GTCTC(1/5)
156 GTCTC
BstAPI 1023 GCAGCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1567 GCAGAACTTGC
BstXI 348 CCATAAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1544 CCAAGTCACTGG
DraII 870 AGGCCCT [GA]G^G[ACGT]CC[CT]
1554 GGGACCC
EarI 105 GAAGAG CTCTTC(1/4)
1222 GAAGAG
EcoO109I 870 AGGCCCT [GA]G^G[ACGT]CC[CT]
1554 GGGACCC
Ksp632I 105 GAAGAG CTCTTC(1/4)
1222 GAAGAG
MaeII 40 ACGT A^CGT
1761 ACGT
SfaNI 691 GCATC GCATC(5/9)
1121 GCATC
SfcI 1030 CTGCAG C^T[GA][CT]AG
1775 CTACAG
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AfaI AluI AlwI BalI BbvI
BmyI BsaJI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI EaeI EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI PvuII RsaI Sau3AI
Sau96I ScrFI SspI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII