Restriction map for
pF1KE0993
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII Acc65I AccI AccIII AclI
AgeI Alw44I Aor51HI ApaI ApaLI
AscI Asp718I AspHI AspI AvaI
AviII AvrII BanII BbeI BbrPI
BcgI BcnI BglI BlnI BlpI
Bpu1102I BsaBI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsoBI BspDI BspEI BspHI BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
CelII CfoI Cfr10I ClaI CpoI
DraII DraIII DrdI EagI EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRI FauI FseI FspI HaeII
HapII HhaI HinP1I HindIII HpaI
HpaII KasI KpnI KspI MamI
MluI MroI MspI NaeI NarI
NciI NdeI NgoMIV NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspI StuI
SwaI Tth111I Van91I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccII 1813 CGCG CG^CG
AcyI 1017 GACGCC G[GA]^CG[CT]C
AflII 178 CTTAAG C^TTAAG
AflIII 1652 ACATGT A^C[GA][CT]GT
AseI 952 ATTAAT AT^TAAT
AsnI 952 ATTAAT AT^TAAT
Asp700I 1226 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 1833 GGTGCC G^G[CT][GA]CC
BbvCI 1038 GCTGAGG CCTCAGC(-5/-2)
BclI 1895 TGATCA T^GATCA
BfrI 178 CTTAAG C^TTAAG
BmyI 992 GGGCAC G[AGT]GC[ACT]^C
BsaAI 85 TACGTG [CT]AC^GT[GA]
BsaHI 1017 GACGCC G[GA]^CG[CT]C
Bsp1286I 992 GGGCAC G[AGT]GC[ACT]^C
Bsp1407I 219 TGTACA T^GTACA
BspLU11I 1652 ACATGT A^CATGT
BsrGI 219 TGTACA T^GTACA
BstUI 1813 CGCG CG^CG
BstXI 1583 CCAGGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
FbaI 1895 TGATCA T^GATCA
HgaI 1017 GACGC GACGC(5/10)
Hin1I 1017 GACGCC G[GA]^CG[CT]C
HincII 436 GTTGAC GT[CT]^[GA]AC
HindII 436 GTTGAC GT[CT]^[GA]AC
MaeII 86 ACGT A^CGT
MfeI 1361 CAATTG C^AATTG
MunI 1361 CAATTG C^AATTG
MvnI 1813 CGCG CG^CG
NheI 1795 GCTAGC G^CTAGC
PshBI 952 ATTAAT AT^TAAT
PstI 979 CTGCAG CTGCA^G
SapI 1882 GAAGAGC GCTCTTC(1/4)
SphI 655 GCATGC GCATG^C
SspBI 219 TGTACA T^GTACA
XbaI 1617 TCTAGA T^CTAGA
XmnI 1226 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AocI 601 CCTGAGG CC^T[ACGT]AGG
1219 CCTGAGG
BciVI 458 GGATAC GTATCC(6/5)
1390 GGATAC
BfaI 1618 CTAG C^TAG
1796 CTAG
BglII 342 AGATCT A^GATCT
1135 AGATCT
BpmI 1048 CTCCAG CTGGAG(16/14)
1921 CTCCAG
BsmAI 470 GAGAC GTCTC(1/5)
1320 GTCTC
BsmFI 1738 GGGAC GGGAC(10/14)
1656 GTCCC
BspMI 977 ACCTGC ACCTGC(4/8)
1310 ACCTGC
BsrI 577 ACTGG ACTGG(1/-1)
651 ACTGG
Bsu36I 601 CCTGAGG CC^T[ACGT]AGG
1219 CCTGAGG
DsaI 1337 CCATGG C^C[GA][CT]GG
1640 CCATGG
Eco81I 601 CCTGAGG CC^T[ACGT]AGG
1219 CCTGAGG
EcoRV 764 GATATC GAT^ATC
1567 GATATC
MaeI 1618 CTAG C^TAG
1796 CTAG
MspA1I 421 CAGCTG C[AC]G^C[GT]G
1153 CAGCTG
MwoI 1038 GCTGAGGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1795 GCTAGCGAAGC
NcoI 1337 CCATGG C^CATGG
1640 CCATGG
PvuII 421 CAGCTG CAG^CTG
1153 CAGCTG
ScaI 326 AGTACT AGT^ACT
1430 AGTACT
SfcI 871 CTACAG C^T[GA][CT]AG
979 CTGCAG
XcmI 1631 CCATCACCACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1724 CCATGCAGAAGCTGG
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# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AcsI AfaI AhdI AluI
AlwI AlwNI ApoI AspEI AvaII
BalI BamHI BbsI BbvI BpuAI
BsaJI BseRI BsiYI BslI BsmI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI EaeI
Eam1105I EarI EcoRII EcoT14I EcoT22I
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MvaI NdeII NlaIII NlaIV
NsiI NspI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII