Restriction map for
pF1KB7533
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# NO CUT ENZYMES ( 172 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AgeI
AhdI Alw44I AlwNI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaHI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspMI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Cac8I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HindIII HpaI
KasI KpnI KspI MaeI MaeII
MaeIII MfeI MluI MluNI MroI
MscI MseI MslI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI TaqI Tru9I
Tsp45I Tth111I TthHB8I Van91I XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AciI 502 GCGG CCGC(-3/-1)
AcsI 389 AAATTT [GA]^AATT[CT]
AflIII 403 ACATGT A^C[GA][CT]GT
AluI 25 AGCT AG^CT
AocI 142 CCTCAGG CC^T[ACGT]AGG
ApaI 40 GGGCCC GGGCC^C
ApoI 389 AAATTT [GA]^AATT[CT]
Asp700I 9 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 111 CCCGGG C^[CT]CG[GA]G
AvaII 440 GGACC G^G[AT]CC
BanII 40 GGGCCC G[GA]GC[CT]^C
BbsI 226 GAAGAC GAAGAC(2/6)
BglI 108 GCCCCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 40 GGGCCC G[AGT]GC[ACT]^C
BpuAI 226 GAAGAC GAAGAC(2/6)
BsaBI 488 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 421 CTCCTC GAGGAG(10/8)
BsmFI 357 GTCCC GGGAC(10/14)
BsoBI 111 CCCGGG C^[CT]CG[GA]G
Bsp1286I 40 GGGCCC G[AGT]GC[ACT]^C
BspLU11I 403 ACATGT A^CATGT
BsrBI 500 GAGCGG CCGCTC(-3/-3)
BstYI 345 AGATCC [GA]^GATC[CT]
Bsu36I 142 CCTCAGG CC^T[ACGT]AGG
DraII 40 GGGCCCC [GA]G^G[ACGT]CC[CT]
EaeI 317 CGGCCA [CT]^GGCC[GA]
EarI 232 GAAGAG CTCTTC(1/4)
Eco81I 142 CCTCAGG CC^T[ACGT]AGG
EcoO109I 40 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 269 CCAAGG C^C[AT][AT]GG
HapII 112 CCGG C^CGG
HincII 399 GTCAAC GT[CT]^[GA]AC
HindII 399 GTCAAC GT[CT]^[GA]AC
HpaII 112 CCGG C^CGG
HphI 266 TCACC GGTGA(8/7)
Ksp632I 232 GAAGAG CTCTTC(1/4)
MamI 488 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 345 AGATCC [GA]^GATC[CT]
MspA1I 24 CAGCTG C[AC]G^C[GT]G
MspI 112 CCGG C^CGG
MwoI 108 GCCCCCGGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 403 ACATGT [GA]CATG^[CT]
PvuII 24 CAGCTG CAG^CTG
SmaI 111 CCCGGG CCC^GGG
SspI 507 AATATT AAT^ATT
StyI 269 CCAAGG C^C[AT][AT]GG
XhoII 345 AGATCC [GA]^GATC[CT]
XmaCI 111 CCCGGG C^CCGGG
XmaI 111 CCCGGG C^CCGGG
XmnI 9 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 9 enzymes) #
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AlwI 346 GATCC GGATC(4/5)
487 GGATC
BbvI 238 GCAGC GCAGC(8/12)
31 GCTGC
BcnI 111 CCCGG CC^[GC]GG
112 CCGGG
BsgI 193 CTGCAC GTGCAG(16/14)
65 GTGCAG
BsrI 186 ACTGG ACTGG(1/-1)
323 ACTGG
HinfI 472 GAATC G^A[ACGT]TC
493 GAATC
NciI 111 CCCGG CC^[GC]GG
112 CCGGG
TfiI 472 GAATC G^A[AT]TC
493 GAATC
TseI 31 GCTGC G^C[AT]GC
238 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
BsaJI BsiYI BslI BssKI BstF5I
BstNI Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII ItaI MboI
MboII MnlI MvaI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
Tsp509I TspRI