Restriction map for
pF1KB7547
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# NO CUT ENZYMES ( 180 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcyI AfaI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BmyI
BpmI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI Bsp1286I
Bsp1407I BspEI BspHI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstEII BstF5I BstPI BstUI
BstXI BstYI BstZ17I Bsu36I Cfr10I
CpoI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FokI FseI FspI HapII
HgaI Hin1I HincII HindII HindIII
HpaI HpaII KasI KpnI Ksp632I
KspI MaeII MamI MflI MluI
MluNI MroI MscI MslI MspA1I
MspI MvnI MwoI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfoI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tsp45I Tth111I Van91I XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 176 GTCTAC GT^[AC][GT]AC
AcsI 531 AAATTT [GA]^AATT[CT]
AflIII 180 ACATGT A^C[GA][CT]GT
AlwI 225 GGATC GGATC(4/5)
AlwNI 126 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 531 AAATTT [GA]^AATT[CT]
AseI 111 ATTAAT AT^TAAT
AsnI 111 ATTAAT AT^TAAT
AvaI 31 CTCGGG C^[CT]CG[GA]G
BbvI 146 GCAGC GCAGC(8/12)
BlpI 25 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 25 GCTCAGC GC^T[ACGT]AGC
BsmAI 3 GTCTC GTCTC(1/5)
BsoBI 31 CTCGGG C^[CT]CG[GA]G
BspDI 403 ATCGAT AT^CGAT
BspLU11I 180 ACATGT A^CATGT
BspMI 142 ACCTGC ACCTGC(4/8)
BstBI 252 TTCGAA TT^CGAA
Cac8I 477 GCGTGC GC[ACGT]^[ACGT]GC
CelII 25 GCTCAGC GC^T[ACGT]AGC
CfoI 23 GCGC GCG^C
ClaI 403 ATCGAT AT^CGAT
DpnII 226 GATC ^GATC
Fnu4HI 146 GCAGC GC^[ACGT]GC
HaeII 22 GGCGCT [GA]GCGC^[CT]
HhaI 23 GCGC GCG^C
HinP1I 23 GCGC G^CGC
HphI 228 TCACC GGTGA(8/7)
ItaI 146 GCAGC GC^[ACGT]GC
MaeIII 167 GTAAC ^GT[ACGT]AC
MboI 226 GATC ^GATC
MfeI 65 CAATTG C^AATTG
MseI 112 TTAA T^TAA
MunI 65 CAATTG C^AATTG
NdeII 226 GATC ^GATC
NlaIII 181 CATG CATG^
NlaIV 494 GGTCCC GG[ACGT]^[ACGT]CC
NspI 180 ACATGT [GA]CATG^[CT]
NspV 252 TTCGAA TT^CGAA
PpuMI 493 AGGTCCC [GA]G^G[AT]CC[CT]
PshBI 111 ATTAAT AT^TAAT
PstI 144 CTGCAG CTGCA^G
Sau3AI 226 GATC ^GATC
SfcI 144 CTGCAG C^T[GA][CT]AG
SfuI 252 TTCGAA TT^CGAA
Tru9I 112 TTAA T^TAA
TseI 146 GCAGC G^C[AT]GC
TspRI 198 CAGTG CA[GC]TG[ACGT][ACGT]^
XbaI 438 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AvaII 87 GGACC G^G[AT]CC
494 GGTCC
BfaI 215 CTAG C^TAG
439 CTAG
BsaJI 130 CCCTGG C^C[ACGT][ACGT]GG
131 CCTGGG
BsiYI 193 CCTCCCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
213 CCCTAGCGTGG
BslI 193 CCTCCCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
213 CCCTAGCGTGG
BsmFI 184 GTCCC GGGAC(10/14)
495 GTCCC
BsrI 84 ACTGG ACTGG(1/-1)
197 CCAGT
BssKI 131 CCTGG ^CC[ACGT]GG
397 CCTGG
BstNI 131 CCTGG CC^[AT]GG
397 CCTGG
DraII 127 AGGCCCT [GA]G^G[ACGT]CC[CT]
493 AGGTCCC
EcoO109I 127 AGGCCCT [GA]G^G[ACGT]CC[CT]
493 AGGTCCC
EcoRII 131 CCTGG ^CC[AT]GG
397 CCTGG
HaeIII 128 GGCC GG^CC
322 GGCC
MaeI 215 CTAG C^TAG
439 CTAG
MvaI 131 CCTGG CC^[AT]GG
397 CCTGG
ScrFI 131 CCTGG CC^[ACGT]GG
397 CCTGG
TaqI 253 TCGA T^CGA
404 TCGA
TthHB8I 253 TCGA T^CGA
404 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
AluI BbsI BpuAI Cfr13I DdeI
HinfI MboII MnlI Sau96I TfiI
Tsp509I