Restriction map for
pF1KB5856
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BcgI BfrI BglI BglII BlnI
BsaAI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsiYI BslI
BsmI BspDI BspEI BspMI BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CfoI Cfr10I ClaI CpoI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoT14I FauI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI MluI MluNI MroI MscI
MwoI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PstI
PvuI RsrII SalI SbfI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI Sse8387I StuI
StyI SwaI Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AccII 63 CGCG CG^CG
AseI 817 ATTAAT AT^TAAT
AsnI 817 ATTAAT AT^TAAT
AspHI 1330 GAGCTC G[AT]GC[AT]^C
AspI 1090 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1098 CCCGGG C^[CT]CG[GA]G
BanI 58 GGCACC G^G[CT][GA]CC
BanII 1330 GAGCTC G[GA]GC[CT]^C
BbsI 1009 GAAGAC GAAGAC(2/6)
BciVI 616 GTATCC GTATCC(6/5)
BlpI 780 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 780 GCTTAGC GC^T[ACGT]AGC
BpuAI 1009 GAAGAC GAAGAC(2/6)
BsaBI 478 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 1330 GAGCTC G[AT]GC[AT]^C
BsmBI 461 GAGACG CGTCTC(1/5)
BsoBI 1098 CCCGGG C^[CT]CG[GA]G
Bsp1407I 512 TGTACA T^GTACA
BspHI 422 TCATGA T^CATGA
BsrGI 512 TGTACA T^GTACA
BstF5I 1404 GGATG GGATG(2/0)
BstUI 63 CGCG CG^CG
BstXI 1247 CCAGCTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 780 GCTTAGC GC^T[ACGT]AGC
DraI 1179 TTTAAA TTT^AAA
DraII 164 AGGTCCC [GA]G^G[ACGT]CC[CT]
DsaI 62 CCGCGG C^C[GA][CT]GG
EarI 967 GAAGAG CTCTTC(1/4)
EcoO109I 164 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRV 397 GATATC GAT^ATC
EcoT22I 1000 ATGCAT ATGCA^T
FokI 1404 GGATG GGATG(9/13)
HaeIII 74 GGCC GG^CC
HapII 1099 CCGG C^CGG
HpaII 1099 CCGG C^CGG
Ksp632I 967 GAAGAG CTCTTC(1/4)
KspI 62 CCGCGG CCGC^GG
MamI 478 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 107 CAATTG C^AATTG
MspI 1099 CCGG C^CGG
MunI 107 CAATTG C^AATTG
MvnI 63 CGCG CG^CG
NsiI 1000 ATGCAT ATGCA^T
PflFI 1090 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 164 AGGTCCC [GA]G^G[AT]CC[CT]
PshBI 817 ATTAAT AT^TAAT
PvuII 1248 CAGCTG CAG^CTG
RcaI 422 TCATGA T^CATGA
SacI 1330 GAGCTC GAGCT^C
SacII 62 CCGCGG CCGC^GG
SapI 967 GAAGAGC GCTCTTC(1/4)
ScaI 635 AGTACT AGT^ACT
SmaI 1098 CCCGGG CCC^GGG
SphI 998 GCATGC GCATG^C
SspBI 512 TGTACA T^GTACA
TaqI 476 TCGA T^CGA
Tth111I 1090 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 476 TCGA T^CGA
XcmI 1155 CCAGTCTGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1098 CCCGGG C^CCGGG
XmaI 1098 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AflIII 663 ACATGT A^C[GA][CT]GT
1380 ACATGT
Asp700I 1078 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1199 GAAATTGTTC
BclI 264 TGATCA T^GATCA
1215 TGATCA
BcnI 1098 CCCGG CC^[GC]GG
1099 CCGGG
BmyI 57 GGGCAC G[AGT]GC[ACT]^C
1330 GAGCTC
BseRI 7 CTCCTC GAGGAG(10/8)
409 GAGGAG
BsmFI 166 GTCCC GGGAC(10/14)
1096 GTCCC
Bsp1286I 57 GGGCAC G[AGT]GC[ACT]^C
1330 GAGCTC
BspLU11I 663 ACATGT A^CATGT
1380 ACATGT
Cac8I 226 GCCTGC GC[ACGT]^[ACGT]GC
998 GCATGC
EcoRI 207 GAATTC G^AATTC
627 GAATTC
FbaI 264 TGATCA T^GATCA
1215 TGATCA
MaeII 925 ACGT A^CGT
1091 ACGT
MslI 268 CATGTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
504 CATTAGGGTG
MspA1I 62 CCGCGG C[AC]G^C[GT]G
1248 CAGCTG
NciI 1098 CCCGG CC^[GC]GG
1099 CCGGG
Tsp45I 584 GTGAC ^GT[GC]AC
1066 GTGAC
XmnI 1078 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1199 GAAATTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbvI BfaI BpmI
BsaJI BsmAI BsrI BssKI BstNI
BstYI Cfr13I DdeI DpnII EcoRII
Fnu4HI HinfI HphI ItaI MaeI
MaeIII MboI MboII MflI MnlI
MseI MvaI NdeII NlaIII NlaIV
NspI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SspI TfiI Tru9I
TseI Tsp509I TspRI XhoII