Restriction map for
pF1KB5961
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# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmI BsoBI Bsp1407I
BspEI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I CpoI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HaeII HgaI
Hin1I HincII HindII HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI StyI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 23 enzymes) #
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Asp700I 700 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 338 GTGCTC G[AT]GC[AT]^C
BanI 64 GGTGCC G^G[CT][GA]CC
BanII 78 GGGCTC G[GA]GC[CT]^C
BsiHKAI 338 GTGCTC G[AT]GC[AT]^C
BspDI 1277 ATCGAT AT^CGAT
BspHI 767 TCATGA T^CATGA
BspMI 486 GCAGGT ACCTGC(4/8)
BstNI 68 CCAGG CC^[AT]GG
ClaI 1277 ATCGAT AT^CGAT
DdeI 754 CTCAG C^T[ACGT]AG
DsaI 120 CCACGG C^C[GA][CT]GG
EarI 1080 CTCTTC CTCTTC(1/4)
EcoRII 68 CCAGG ^CC[AT]GG
HindIII 1198 AAGCTT A^AGCTT
Ksp632I 1080 CTCTTC CTCTTC(1/4)
MaeII 1368 ACGT A^CGT
MslI 727 CACCAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 68 CCAGG CC^[AT]GG
RcaI 767 TCATGA T^CATGA
SmlI 1145 CTCAAG C^T[CT][GA]AG
SspI 436 AATATT AAT^ATT
XmnI 700 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
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AfaI 275 GTAC GT^AC
1063 GTAC
AvaII 170 GGTCC G^G[AT]CC
662 GGACC
BcnI 18 CCGGG CC^[GC]GG
86 CCCGG
BglI 49 GCCCAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
547 GCCATTTTGGC
BmyI 78 GGGCTC G[AGT]GC[ACT]^C
338 GTGCTC
BpmI 101 CTGGAG CTGGAG(16/14)
448 CTGGAG
BsaJI 68 CCAGGG C^C[ACGT][ACGT]GG
120 CCACGG
BsmBI 104 GAGACG CGTCTC(1/5)
199 GAGACG
BsmFI 224 GGGAC GGGAC(10/14)
661 GGGAC
Bsp1286I 78 GGGCTC G[AGT]GC[ACT]^C
338 GTGCTC
BstF5I 210 GGATG GGATG(2/0)
845 GGATG
BstYI 877 GGATCT [GA]^GATC[CT]
1324 GGATCT
DraI 745 TTTAAA TTT^AAA
882 TTTAAA
FokI 210 GGATG GGATG(9/13)
845 GGATG
MflI 877 GGATCT [GA]^GATC[CT]
1324 GGATCT
MspA1I 165 CAGCGG C[AC]G^C[GT]G
235 CAGCGG
NciI 18 CCGGG CC^[GC]GG
86 CCCGG
PleI 193 GACTC GAGTC(4/5)
723 GACTC
PpuMI 169 GGGTCCT [GA]G^G[AT]CC[CT]
661 GGGACCT
PstI 484 CTGCAG CTGCA^G
571 CTGCAG
RsaI 275 GTAC GT^AC
1063 GTAC
Tsp45I 185 GTCAC ^GT[GC]AC
323 GTGAC
XhoII 877 GGATCT [GA]^GATC[CT]
1324 GGATCT
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AluI AlwI BbvI BfaI
BseRI BsiYI BslI BsmAI BsrI
BssKI Cac8I CfoI Cfr13I DpnII
DraII EcoO109I FauI Fnu4HI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MnlI MseI MspI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I