Restriction map for
pF1KE0606
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflIII AgeI AhdI Alw44I ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AvrII BamHI BbsI
BglII BlnI BlpI Bpu1102I BpuAI
BsaWI BseAI BsiEI BsiWI BsmI
BspDI BspEI BspLU11I BsrDI BsrFI
BssHII Bst1107I BstBI BstXI BstYI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco52I EcoRI EcoRV EcoT22I FseI
HincII HindII HindIII HpaI KpnI
KspI MfeI MflI MluI MroI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI XbaI XcmI XhoII
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 688 GTCTAC GT^[AC][GT]AC
AflII 1227 CTTAAG C^TTAAG
AocI 852 CCTGAGG CC^T[ACGT]AGG
Aor51HI 395 AGCGCT AGC^GCT
Asp700I 1218 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 742 GTGCTC G[AT]GC[AT]^C
AviII 1131 TGCGCA TGC^GCA
BalI 74 TGGCCA TGG^CCA
BbrPI 373 CACGTG CAC^GTG
BcgI 1044 GCAGCAGTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 184 GGATAC GTATCC(6/5)
BfaI 1291 CTAG C^TAG
BfrI 1227 CTTAAG C^TTAAG
BsaAI 373 CACGTG [CT]AC^GT[GA]
BsaBI 84 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 973 CTCCTC GAGGAG(10/8)
BsiHKAI 742 GTGCTC G[AT]GC[AT]^C
BsmBI 988 GAGACG CGTCTC(1/5)
Bsp1407I 1028 TGTACA T^GTACA
BspHI 746 TCATGA T^CATGA
BsrGI 1028 TGTACA T^GTACA
BstAPI 23 GCAGTGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 887 GGATG GGATG(2/0)
Bsu36I 852 CCTGAGG CC^T[ACGT]AGG
DraIII 211 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 395 AGCGCT AGC^GCT
Eco81I 852 CCTGAGG CC^T[ACGT]AGG
FokI 887 GGATG GGATG(9/13)
FspI 1131 TGCGCA TGC^GCA
HgaI 1183 GCGTC GACGC(5/10)
MaeI 1291 CTAG C^TAG
MamI 84 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 74 TGGCCA TGG^CCA
MscI 74 TGGCCA TGG^CCA
MseI 1228 TTAA T^TAA
PaeR7I 1282 CTCGAG C^TCGAG
PflMI 641 CCAGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 373 CACGTG CAC^GTG
PmlI 373 CACGTG CAC^GTG
PvuII 793 CAGCTG CAG^CTG
RcaI 746 TCATGA T^CATGA
SapI 1176 GCTCTTC GCTCTTC(1/4)
SmaI 18 CCCGGG CCC^GGG
SphI 257 GCATGC GCATG^C
SspBI 1028 TGTACA T^GTACA
StuI 347 AGGCCT AGG^CCT
Tru9I 1228 TTAA T^TAA
Tsp509I 1321 AATT ^AATT
Van91I 641 CCAGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1282 CTCGAG C^TCGAG
XmaCI 18 CCCGGG C^CCGGG
XmaI 18 CCCGGG C^CCGGG
XmnI 1218 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AcyI 676 GGCGCC G[GA]^CG[CT]C
1190 GGCGCC
AspI 297 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
942 GACGAGGTC
BanI 676 GGCGCC G^G[CT][GA]CC
1190 GGCGCC
BbeI 676 GGCGCC GGCGC^C
1190 GGCGCC
BbvCI 1002 CCTCAGC CCTCAGC(-5/-2)
795 GCTGAGG
BclI 45 TGATCA T^GATCA
113 TGATCA
BpmI 579 CTCCAG CTGGAG(16/14)
639 CTCCAG
BsaHI 676 GGCGCC G[GA]^CG[CT]C
1190 GGCGCC
BsaI 607 GAGACC GGTCTC(1/5)
562 GGTCTC
BspMI 738 GCAGGT ACCTGC(4/8)
1068 GCAGGT
BsrBI 576 CCGCTC CCGCTC(-3/-3)
1021 CCGCTC
BstEII 159 GGTCACC G^GT[ACGT]ACC
480 GGTCACC
BstPI 159 GGTCACC G^GT[ACGT]ACC
480 GGTCACC
DraII 441 GGGACCT [GA]G^G[ACGT]CC[CT]
946 AGGTCCT
DsaI 263 CCATGG C^C[GA][CT]GG
518 CCATGG
EcoNI 339 CCTCTTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1199 CCTCTGCAAGG
EcoO109I 441 GGGACCT [GA]G^G[ACGT]CC[CT]
946 AGGTCCT
EcoO65I 159 GGTCACC G^GT[ACGT]ACC
480 GGTCACC
EcoT14I 263 CCATGG C^C[AT][AT]GG
518 CCATGG
FbaI 45 TGATCA T^GATCA
113 TGATCA
Hin1I 676 GGCGCC G[GA]^CG[CT]C
1190 GGCGCC
KasI 676 GGCGCC G^GCGCC
1190 GGCGCC
MspA1I 645 CCGCTG C[AC]G^C[GT]G
793 CAGCTG
NarI 676 GGCGCC GG^CGCC
1190 GGCGCC
NcoI 263 CCATGG C^CATGG
518 CCATGG
NspI 257 GCATGC [GA]CATG^[CT]
1128 ACATGC
PflFI 297 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
942 GACGAGGTC
PleI 1278 GAGTC GAGTC(4/5)
1285 GAGTC
PpuMI 441 GGGACCT [GA]G^G[AT]CC[CT]
946 AGGTCCT
SfoI 676 GGCGCC GGC^GCC
1190 GGCGCC
SmlI 1227 CTTAAG C^T[CT][GA]AG
1282 CTCGAG
StyI 263 CCATGG C^C[AT][AT]GG
518 CCATGG
TaqI 344 TCGA T^CGA
1283 TCGA
TfiI 288 GAATC G^A[AT]TC
900 GATTC
TspRI 24 CAGTG CA[GC]TG[ACGT][ACGT]^
500 CACTG
Tth111I 297 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
942 GACGAGGTC
TthHB8I 344 TCGA T^CGA
1283 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AvaI AvaII BanII BbvI
BcnI BglI BmyI BsaJI BsgI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BssSI BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII EaeI EarI EcoRII FauI
Fnu4HI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MslI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TseI Tsp45I