Restriction map for
pF1KB6655
##################################
# NO CUT ENZYMES ( 137 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AflII AflIII AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BfrI BlnI BlpI
BmyI Bpu1102I BpuAI BsaBI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstZ17I CelII CfoI ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI FseI FspI
HaeII HgaI HhaI HinP1I HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 58 GACGTC GACGT^C
AcsI 684 GAATTC [GA]^AATT[CT]
AcyI 58 GACGTC G[GA]^CG[CT]C
AfaI 165 GTAC GT^AC
AgeI 1001 ACCGGT A^CCGGT
AocI 678 CCTTAGG CC^T[ACGT]AGG
ApoI 684 GAATTC [GA]^AATT[CT]
BciVI 1153 GTATCC GTATCC(6/5)
BfaI 922 CTAG C^TAG
BglI 1120 GCCAGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 872 AGATCT A^GATCT
BsaAI 166 TACGTG [CT]AC^GT[GA]
BsaHI 58 GACGTC G[GA]^CG[CT]C
BsaWI 1001 ACCGGT [AT]^CCGG[AT]
BsmBI 417 CGTCTC CGTCTC(1/5)
BsmFI 674 GGGAC GGGAC(10/14)
BsmI 547 GCATTC GAATGC(1/-1)
BspMI 116 ACCTGC ACCTGC(4/8)
BsrDI 188 GCAATG GCAATG(2/0)
BsrFI 1001 ACCGGT [GA]^CCGG[CT]
BstEII 997 GGTTACC G^GT[ACGT]ACC
BstPI 997 GGTTACC G^GT[ACGT]ACC
Bsu36I 678 CCTTAGG CC^T[ACGT]AGG
Cfr10I 1001 ACCGGT [GA]^CCGG[CT]
DraIII 691 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 47 CCATGG C^C[GA][CT]GG
Eco81I 678 CCTTAGG CC^T[ACGT]AGG
EcoO65I 997 GGTTACC G^GT[ACGT]ACC
EcoRI 684 GAATTC G^AATTC
EcoT14I 47 CCATGG C^C[AT][AT]GG
FauI 639 GCGGG CCCGC(4/6)
Hin1I 58 GACGTC G[GA]^CG[CT]C
HincII 1045 GTTGAC GT[CT]^[GA]AC
HindII 1045 GTTGAC GT[CT]^[GA]AC
MaeI 922 CTAG C^TAG
NcoI 47 CCATGG C^CATGG
PflMI 690 CCACATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1001 ACCGGT A^CCGGT
PvuII 886 CAGCTG CAG^CTG
RsaI 165 GTAC GT^AC
SapI 1213 GAAGAGC GCTCTTC(1/4)
SfaNI 560 GATGC GCATC(5/9)
StyI 47 CCATGG C^C[AT][AT]GG
Van91I 690 CCACATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 893 CCAAAGAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AciI 826 CCGC CCGC(-3/-1)
639 GCGG
AlwI 987 GATCC GGATC(4/5)
51 GGATC
BalI 2 TGGCCA TGG^CCA
74 TGGCCA
BbvI 888 GCTGC GCAGC(8/12)
1134 GCTGC
BclI 722 TGATCA T^GATCA
830 TGATCA
BcnI 471 CCGGG CC^[GC]GG
666 CCGGG
BstXI 245 CCACATTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1100 CCAAAGAGCTGG
BstYI 872 AGATCT [GA]^GATC[CT]
986 AGATCC
DraII 374 AGGACCC [GA]G^G[ACGT]CC[CT]
674 GGGACCT
EcoO109I 374 AGGACCC [GA]G^G[ACGT]CC[CT]
674 GGGACCT
EcoRV 235 GATATC GAT^ATC
775 GATATC
EcoT22I 545 ATGCAT ATGCA^T
561 ATGCAT
FbaI 722 TGATCA T^GATCA
830 TGATCA
Fnu4HI 888 GCTGC GC^[ACGT]GC
1134 GCTGC
ItaI 888 GCTGC GC^[ACGT]GC
1134 GCTGC
MflI 872 AGATCT [GA]^GATC[CT]
986 AGATCC
MluNI 2 TGGCCA TGG^CCA
74 TGGCCA
MscI 2 TGGCCA TGG^CCA
74 TGGCCA
MseI 514 TTAA T^TAA
848 TTAA
MslI 246 CACATTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
942 CACAGGGATG
MspA1I 826 CCGCTG C[AC]G^C[GT]G
886 CAGCTG
NciI 471 CCGGG CC^[GC]GG
666 CCGGG
NsiI 545 ATGCAT ATGCA^T
561 ATGCAT
PpuMI 374 AGGACCC [GA]G^G[AT]CC[CT]
674 GGGACCT
PshAI 24 GACTAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
603 GACCCTGGTC
Tru9I 514 TTAA T^TAA
848 TTAA
TseI 888 GCTGC G^C[AT]GC
1134 GCTGC
Tsp45I 304 GTGAC ^GT[GC]AC
822 GTCAC
XhoII 872 AGATCT [GA]^GATC[CT]
986 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AluI AvaII BpmI BsaI BsaJI
BsiYI BslI BsmAI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EaeI EarI EcoRII FokI
HaeIII HapII HinfI HpaII HphI
Ksp632I MaeII MaeIII MboI MboII
MnlI MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI Sau3AI
Sau96I ScrFI TaqI TfiI Tsp509I
TspRI TthHB8I