Restriction map for
pF1KB6689
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# NO CUT ENZYMES ( 153 enzymes) #
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Acc65I AccIII AclI AcsI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI Asp700I
Asp718I AspEI AspHI AviII AvrII
BalI BanI BbeI BbrPI BbvCI
BciVI BclI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII CfoI ClaI CpoI
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I FauI FbaI FseI FspI
HaeII HhaI HinP1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MamI MfeI MluI MluNI
MroI MscI MslI MspA1I MunI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
StuI StyI SwaI Van91I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 980 GACGTC GACGT^C
AccI 250 GTAGAC GT^[AC][GT]AC
AccII 786 CGCG CG^CG
AciI 787 GCGG CCGC(-3/-1)
AcyI 980 GACGTC G[GA]^CG[CT]C
AfaI 201 GTAC GT^AC
AlwNI 917 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 4 GGGCCC GGGCC^C
AseI 823 ATTAAT AT^TAAT
AsnI 823 ATTAAT AT^TAAT
AspI 685 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 338 CCCGGG C^[CT]CG[GA]G
BbvI 435 GCTGC GCAGC(8/12)
BcgI 954 CGAAGTCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 392 CTAG C^TAG
BsaHI 980 GACGTC G[GA]^CG[CT]C
BseRI 395 GAGGAG GAGGAG(10/8)
BsgI 24 CTGCAC GTGCAG(16/14)
BsmAI 958 GTCTC GTCTC(1/5)
BsoBI 338 CCCGGG C^[CT]CG[GA]G
BspMI 86 ACCTGC ACCTGC(4/8)
BsrFI 801 ACCGGC [GA]^CCGG[CT]
BssSI 621 CACGAG CACGAG(-5/-1)
BstUI 786 CGCG CG^CG
Cfr10I 801 ACCGGC [GA]^CCGG[CT]
DraI 72 TTTAAA TTT^AAA
EcoT22I 311 ATGCAT ATGCA^T
Fnu4HI 435 GCTGC GC^[ACGT]GC
HgaI 346 GACGC GACGC(5/10)
Hin1I 980 GACGTC G[GA]^CG[CT]C
ItaI 435 GCTGC GC^[ACGT]GC
MaeI 392 CTAG C^TAG
MaeII 981 ACGT A^CGT
MvnI 786 CGCG CG^CG
NdeI 242 CATATG CA^TATG
NsiI 311 ATGCAT ATGCA^T
NspI 674 GCATGC [GA]CATG^[CT]
PflFI 685 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 823 ATTAAT AT^TAAT
RsaI 201 GTAC GT^AC
SmaI 338 CCCGGG CCC^GGG
SmlI 101 CTCAAG C^T[CT][GA]AG
SphI 674 GCATGC GCATG^C
TaqI 953 TCGA T^CGA
TseI 435 GCTGC G^C[AT]GC
Tth111I 685 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 953 TCGA T^CGA
XmaCI 338 CCCGGG C^CCGGG
XmaI 338 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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BamHI 352 GGATCC G^GATCC
599 GGATCC
BanII 4 GGGCCC G[GA]GC[CT]^C
889 GGGCTC
BbsI 343 GAAGAC GAAGAC(2/6)
983 GTCTTC
BmyI 4 GGGCCC G[AGT]GC[ACT]^C
889 GGGCTC
BpuAI 343 GAAGAC GAAGAC(2/6)
983 GTCTTC
BsiYI 41 CCTGGAAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
530 CCTACATTTGG
BslI 41 CCTGGAAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
530 CCTACATTTGG
BsmFI 659 GGGAC GGGAC(10/14)
569 GTCCC
Bsp1286I 4 GGGCCC G[AGT]GC[ACT]^C
889 GGGCTC
BstYI 352 GGATCC [GA]^GATC[CT]
599 GGATCC
HaeIII 5 GGCC GG^CC
275 GGCC
HinfI 180 GAATC G^A[ACGT]TC
790 GAATC
MflI 352 GGATCC [GA]^GATC[CT]
599 GGATCC
NlaIII 675 CATG CATG^
945 CATG
PpuMI 50 GGGTCCT [GA]G^G[AT]CC[CT]
636 AGGACCC
SfaNI 64 GCATC GCATC(5/9)
313 GCATC
TfiI 180 GAATC G^A[AT]TC
790 GAATC
XhoII 352 GGATCC [GA]^GATC[CT]
599 GGATCC
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
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AluI AlwI AvaII BcnI BsaJI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII FokI HapII HpaII HphI
MaeIII MboI MboII MnlI MseI
MspI MvaI MwoI NciI NdeII
NlaIV Sau3AI Sau96I ScrFI Tru9I
Tsp45I Tsp509I TspRI