##################################
# NO CUT ENZYMES (  201 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AcyI        AgeI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanII       BbeI        BbsI        BbvCI       
BcgI        BciVI       BclI        BcnI        BfaI        
BglI        BglII       BlnI        BlpI        BmyI        
Bpu1102I    BpuAI       BsaBI       BsaHI       BsaI        
BsaJI       BsaWI       BseAI       BseRI       BsgI        
BsiHKAI     BsiWI       BsiYI       BslI        BsmBI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstXI       BstYI       BstZ17I     Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      ClaI        CpoI        
DdeI        DraI        DraIII      DrdI        DsaI        
EaeI        EagI        EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HinfI       HpaI        HpaII       
HphI        KasI        Ksp632I     KspI        MaeI        
MaeIII      MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MslI        MspA1I      
MspI        MunI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NlaIII      
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmeI        PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfaNI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TfiI        Tsp45I      
Tsp509I     TspRI       Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

Acc65I        106  GGTACC               G^GTACC
AccII          43  CGCG                 CG^CG
AciI           42  CCGC                 CCGC(-3/-1)
AflII          79  CTTAAG               C^TTAAG
AflIII        161  ACGTGT               A^C[GA][CT]GT
AhdI          157  GACCACGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I       106  GGTACC               G^GTACC
AspEI         157  GACCACGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI          106  GGTACC               G^G[CT][GA]CC
BbrPI         160  CACGTG               CAC^GTG
BbvI           94  GCAGC                GCAGC(8/12)
BfrI           79  CTTAAG               C^TTAAG
BpmI          202  CTGGAG               CTGGAG(16/14)
BsaAI         160  CACGTG               [CT]AC^GT[GA]
BsiEI          21  CGACCG               CG[GA][CT]^CG
BsmAI         165  GTCTC                GTCTC(1/5)
BsrI          103  ACTGG                ACTGG(1/-1)
BssKI         201  CCTGG                ^CC[ACGT]GG
BstNI         201  CCTGG                CC^[AT]GG
BstUI          43  CGCG                 CG^CG
DpnII           3  GATC                 ^GATC
DraII          74  GGGACCT              [GA]G^G[ACGT]CC[CT]
Eam1105I      157  GACCACGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I       74  GGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRII        201  CCTGG                ^CC[AT]GG
Fnu4HI         94  GCAGC                GC^[ACGT]GC
ItaI           94  GCAGC                GC^[ACGT]GC
KpnI          106  GGTACC               GGTAC^C
MaeII         161  ACGT                 A^CGT
MboI            3  GATC                 ^GATC
MnlI            7  GAGG                 CCTC(7/6)
MvaI          201  CCTGG                CC^[AT]GG
MvnI           43  CGCG                 CG^CG
MwoI           44  GCGTTAAATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII           3  GATC                 ^GATC
PmaCI         160  CACGTG               CAC^GTG
PmlI          160  CACGTG               CAC^GTG
PpuMI          74  GGGACCT              [GA]G^G[AT]CC[CT]
Sau3AI          3  GATC                 ^GATC
ScrFI         201  CCTGG                CC^[ACGT]GG
SmlI           79  CTTAAG               C^T[CT][GA]AG
TaqI            5  TCGA                 T^CGA
TseI           94  GCAGC                G^C[AT]GC
TthHB8I         5  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

AfaI          107  GTAC                 GT^AC
              141  GTAC                
AluI           86  AGCT                 AG^CT
              206  AGCT                
BsmFI          74  GGGAC                GGGAC(10/14)
              173  GGGAC               
BstF5I         60  GGATG                GGATG(2/0)
              194  GGATG               
FokI           60  GGATG                GGATG(9/13)
              194  GGATG               
MboII          33  GAAGA                GAAGA(8/7)
              132  GAAGA               
NlaIV          74  GGGACC               GG[ACGT]^[ACGT]CC
              106  GGTACC              
RsaI          107  GTAC                 GT^AC
              141  GTAC                


#########################################
# 3 OR MORE CUT ENZYMES (    5 enzymes) #
#########################################

AvaII       Cfr13I      MseI        Sau96I      Tru9I