##################################
# NO CUT ENZYMES (  218 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        AluI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspHI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BbvI        BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BlnI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaJI       BsaWI       BseAI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsiYI       
BslI        BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrGI       BsrI        
BssHII      BssKI       BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstNI       BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      Cac8I       
CelII       CfoI        ClaI        CpoI        DdeI        
DraI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRII      EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        Fnu4HI      FokI        FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        HphI        
ItaI        KasI        KpnI        KspI        MaeII       
MamI        MfeI        MluI        MluNI       MroI        
MscI        MseI        MslI        MspA1I      MunI        
MvaI        MvnI        MwoI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NlaIV       NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScrFI       SexAI       SfaNI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   27 enzymes) #
###################################

AhdI          134  GACTTGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI         134  GACTTGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BfaI          154  CTAG                 C^TAG
BglII          14  AGATCT               A^GATCT
BsmAI         132  GAGAC                GTCTC(1/5)
BspHI         177  TCATGA               T^CATGA
BsrFI          56  ACCGGC               [GA]^CCGG[CT]
BstYI          14  AGATCT               [GA]^GATC[CT]
Cfr10I         56  ACCGGC               [GA]^CCGG[CT]
Cfr13I          3  GGCCC                G^G[ACGT]CC
Eam1105I      134  GACTTGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HaeIII          3  GGCC                 GG^CC
HapII          57  CCGG                 C^CGG
HpaII          57  CCGG                 C^CGG
MaeI          154  CTAG                 C^TAG
MaeIII         69  GTTAC                ^GT[ACGT]AC
MflI           14  AGATCT               [GA]^GATC[CT]
MspI           57  CCGG                 C^CGG
NspI           60  GCATGT               [GA]CATG^[CT]
PleI          150  GAGTC                GAGTC(4/5)
RcaI          177  TCATGA               T^CATGA
Sau96I          3  GGCCC                G^G[ACGT]CC
ScaI           32  AGTACT               AGT^ACT
TaqI           38  TCGA                 T^CGA
TfiI          174  GATTC                G^A[AT]TC
TthHB8I        38  TCGA                 T^CGA
XhoII          14  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

BseRI         121  GAGGAG               GAGGAG(10/8)
              129  GAGGAG              
DpnII          15  GATC                 ^GATC
              226  GATC                
EarI          215  CTCTTC               CTCTTC(1/4)
              118  GAAGAG              
HinfI         150  GAGTC                G^A[ACGT]TC
              174  GATTC               
Ksp632I       215  CTCTTC               CTCTTC(1/4)
              118  GAAGAG              
MboI           15  GATC                 ^GATC
              226  GATC                
NdeII          15  GATC                 ^GATC
              226  GATC                
Sau3AI         15  GATC                 ^GATC
              226  GATC                


#########################################
# 3 OR MORE CUT ENZYMES (    5 enzymes) #
#########################################

AfaI        MboII       MnlI        NlaIII      RsaI