Restriction map for
pF1KB6714
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# NO CUT ENZYMES ( 199 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AviII
AvrII BalI BamHI BanII BbeI
BbrPI BbsI BbvCI BbvI BcgI
BcnI BfrI BglI BglII BlnI
BlpI BmyI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmFI BsmI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
Fnu4HI FokI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaI
HpaII ItaI KasI KpnI Ksp632I
KspI MaeII MamI MfeI MflI
MluI MluNI MroI MscI MslI
MspA1I MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI TseI TspRI Tth111I Van91I
XcmI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AciI 20 CCGC CCGC(-3/-1)
AcsI 281 AAATTC [GA]^AATT[CT]
AfaI 396 GTAC GT^AC
AluI 317 AGCT AG^CT
AlwNI 343 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 281 AAATTC [GA]^AATT[CT]
Asp700I 277 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 16 GGCACC G^G[CT][GA]CC
BciVI 268 GTATCC GTATCC(6/5)
BclI 105 TGATCA T^GATCA
BpmI 259 CTCCAG CTGGAG(16/14)
BsaJI 357 CCGAGG C^C[ACGT][ACGT]GG
BseRI 200 GAGGAG GAGGAG(10/8)
BsgI 340 GTGCAG GTGCAG(16/14)
BsiYI 27 CCTCATCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 27 CCTCATCGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BssKI 13 CCTGG ^CC[ACGT]GG
BstNI 13 CCTGG CC^[AT]GG
DdeI 120 CTTAG C^T[ACGT]AG
DpnII 106 GATC ^GATC
DraII 344 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoNI 27 CCTCATCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 344 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRII 13 CCTGG ^CC[AT]GG
FbaI 105 TGATCA T^GATCA
HaeIII 345 GGCC GG^CC
HphI 169 GGTGA GGTGA(8/7)
MaeIII 248 GTGAC ^GT[ACGT]AC
MboI 106 GATC ^GATC
MseI 94 TTAA T^TAA
MvaI 13 CCTGG CC^[AT]GG
NdeII 106 GATC ^GATC
NlaIII 151 CATG CATG^
NlaIV 16 GGCACC GG[ACGT]^[ACGT]CC
PshAI 355 GACCGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 396 GTAC GT^AC
Sau3AI 106 GATC ^GATC
ScrFI 13 CCTGG CC^[ACGT]GG
Tru9I 94 TTAA T^TAA
Tsp45I 248 GTGAC ^GT[GC]AC
XbaI 285 TCTAGA T^CTAGA
XmnI 277 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AvaI 179 CTCGAG C^[CT]CG[GA]G
374 CTCGAG
AvaII 213 GGTCC G^G[AT]CC
354 GGACC
BfaI 220 CTAG C^TAG
286 CTAG
BsoBI 179 CTCGAG C^[CT]CG[GA]G
374 CTCGAG
Cac8I 22 GCCAGC GC[ACGT]^[ACGT]GC
342 GCAGGC
MaeI 220 CTAG C^TAG
286 CTAG
MboII 277 GAAGA GAAGA(8/7)
289 GAAGA
MwoI 17 GCACCGCCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
309 GCAGACCAAGC
PaeR7I 179 CTCGAG C^TCGAG
374 CTCGAG
SmlI 179 CTCGAG C^T[CT][GA]AG
374 CTCGAG
Tsp509I 110 AATT ^AATT
282 AATT
XhoI 179 CTCGAG C^TCGAG
374 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 5 enzymes) #
#########################################
Cfr13I MnlI Sau96I TaqI TthHB8I