Restriction map for
pF1KB6745
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# NO CUT ENZYMES ( 182 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmAI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DdeI DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FokI FseI FspI HgaI
Hin1I HincII HindII HindIII HinfI
HpaI KasI KpnI Ksp632I KspI
MaeI MaeII MaeIII MamI MfeI
MflI MluI MluNI MroI MscI
MslI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI StyI
SwaI TaqI TfiI Tsp45I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AcsI 242 AAATTT [GA]^AATT[CT]
AfaI 270 GTAC GT^AC
AlwNI 118 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 88 AGCGCT AGC^GCT
ApoI 242 AAATTT [GA]^AATT[CT]
AspHI 3 GAGCTC G[AT]GC[AT]^C
AvaI 34 CCCGGG C^[CT]CG[GA]G
BanII 3 GAGCTC G[GA]GC[CT]^C
BbsI 132 GAAGAC GAAGAC(2/6)
BclI 179 TGATCA T^GATCA
BmyI 3 GAGCTC G[AGT]GC[ACT]^C
BpmI 172 CTGGAG CTGGAG(16/14)
BpuAI 132 GAAGAC GAAGAC(2/6)
BsiHKAI 3 GAGCTC G[AT]GC[AT]^C
BsiYI 8 CCGCAGCCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 8 CCGCAGCCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI 34 CCCGGG C^[CT]CG[GA]G
Bsp1286I 3 GAGCTC G[AGT]GC[ACT]^C
Bsp1407I 269 TGTACA T^GTACA
BsrGI 269 TGTACA T^GTACA
DpnII 180 GATC ^GATC
DraII 110 GGGACCT [GA]G^G[ACGT]CC[CT]
Eco47III 88 AGCGCT AGC^GCT
EcoO109I 110 GGGACCT [GA]G^G[ACGT]CC[CT]
FauI 264 CCCGC CCCGC(4/6)
FbaI 179 TGATCA T^GATCA
HaeII 88 AGCGCT [GA]GCGC^[CT]
HaeIII 186 GGCC GG^CC
MboI 180 GATC ^GATC
MseI 284 TTAA T^TAA
MspA1I 265 CCGCTG C[AC]G^C[GT]G
NdeII 180 GATC ^GATC
PpuMI 110 GGGACCT [GA]G^G[AT]CC[CT]
PstI 115 CTGCAG CTGCA^G
RsaI 270 GTAC GT^AC
SacI 3 GAGCTC GAGCT^C
Sau3AI 180 GATC ^GATC
SfcI 115 CTGCAG C^T[GA][CT]AG
SmaI 34 CCCGGG CCC^GGG
SspBI 269 TGTACA T^GTACA
Tru9I 284 TTAA T^TAA
XmaCI 34 CCCGGG C^CCGGG
XmaI 34 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 11 enzymes) #
###################################
AciI 8 CCGC CCGC(-3/-1)
265 CCGC
AluI 4 AGCT AG^CT
96 AGCT
BsmFI 110 GGGAC GGGAC(10/14)
152 GTCCC
BspMI 113 ACCTGC ACCTGC(4/8)
254 ACCTGC
Cac8I 85 GCCAGC GC[ACGT]^[ACGT]GC
258 GCAAGC
CfoI 23 GCGC GCG^C
89 GCGC
HhaI 23 GCGC GCG^C
89 GCGC
HinP1I 23 GCGC G^CGC
89 GCGC
HphI 177 GGTGA GGTGA(8/7)
164 TCACC
NlaIV 110 GGGACC GG[ACGT]^[ACGT]CC
190 GGACCC
Tsp509I 243 AATT ^AATT
282 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AvaII BbvI BcnI BsaJI BssKI
BstNI Cfr13I EcoRII Fnu4HI HapII
HpaII ItaI MboII MnlI MspI
MvaI MwoI NciI Sau96I ScrFI
TseI TspRI