Restriction map for
pF1KE0638
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# NO CUT ENZYMES ( 102 enzymes) #
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AatII Acc65I AclI AcsI AflII
AgeI AhdI Aor51HI ApoI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BsaBI
BsaI BsiEI BsiWI BsmBI BspDI
BspHI BspLU11I BspMI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KpnI KspI
MaeI MamI MfeI MluI MseI
MunI NcoI NdeI NheI NotI
NsiI NspV PacI PaeR7I PinAI
PleI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SfiI SfuI SgrAI
SmlI SnaBI SpeI Sse8387I SspI
SwaI TaqI Tru9I TthHB8I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 71 enzymes) #
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AccI 1165 GTCTAC GT^[AC][GT]AC
AccIII 544 TCCGGA T^CCGGA
AcyI 75 GGCGCC G[GA]^CG[CT]C
AflIII 1058 ACACGT A^C[GA][CT]GT
Alw44I 1085 GTGCAC G^TGCAC
AlwNI 809 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 252 CCTGAGG CC^T[ACGT]AGG
ApaI 1332 GGGCCC GGGCC^C
ApaLI 1085 GTGCAC G^TGCAC
AscI 1064 GGCGCGCC GG^CGCGCC
AspI 648 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 943 GGATCC G^GATCC
BbeI 75 GGCGCC GGCGC^C
BbsI 862 GAAGAC GAAGAC(2/6)
BbvCI 108 CCTCAGC CCTCAGC(-5/-2)
BpuAI 862 GAAGAC GAAGAC(2/6)
BsaHI 75 GGCGCC G[GA]^CG[CT]C
BseAI 544 TCCGGA T^CCGGA
BsgI 28 GTGCAG GTGCAG(16/14)
BsmAI 844 GAGAC GTCTC(1/5)
BsmI 128 GCATTC GAATGC(1/-1)
Bsp1407I 572 TGTACA T^GTACA
BspEI 544 TCCGGA T^CCGGA
BsrBI 451 GAGCGG CCGCTC(-3/-3)
BsrDI 1287 CATTGC GCAATG(2/0)
BsrFI 836 GCCGGC [GA]^CCGG[CT]
BsrGI 572 TGTACA T^GTACA
BssHII 1065 GCGCGC G^CGCGC
BstAPI 609 GCAGTGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 684 CATCC GGATG(2/0)
BstYI 943 GGATCC [GA]^GATC[CT]
Bsu36I 252 CCTGAGG CC^T[ACGT]AGG
Cfr10I 836 GCCGGC [GA]^CCGG[CT]
DsaI 704 CCGTGG C^C[GA][CT]GG
EarI 1256 CTCTTC CTCTTC(1/4)
Eco81I 252 CCTGAGG CC^T[ACGT]AGG
EcoT14I 795 CCAAGG C^C[AT][AT]GG
FokI 684 CATCC GGATG(9/13)
HgaI 498 GACGC GACGC(5/10)
Hin1I 75 GGCGCC G[GA]^CG[CT]C
HincII 1153 GTCAAC GT[CT]^[GA]AC
HindII 1153 GTCAAC GT[CT]^[GA]AC
HinfI 170 GAATC G^A[ACGT]TC
KasI 75 GGCGCC G^GCGCC
Ksp632I 1256 CTCTTC CTCTTC(1/4)
MflI 943 GGATCC [GA]^GATC[CT]
MroI 544 TCCGGA T^CCGGA
MspA1I 111 CAGCTG C[AC]G^C[GT]G
NaeI 836 GCCGGC GCC^GGC
NarI 75 GGCGCC GG^CGCC
NgoMIV 836 GCCGGC G^CCGGC
NruI 726 TCGCGA TCG^CGA
NspI 320 GCATGC [GA]CATG^[CT]
PflFI 648 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1055 CCAACACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1268 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 111 CAGCTG CAG^CTG
SapI 1255 GCTCTTC GCTCTTC(1/4)
SexAI 649 ACCTGGT A^CC[AT]GGT
SfaNI 618 GCATC GCATC(5/9)
SfoI 75 GGCGCC GGC^GCC
SphI 320 GCATGC GCATG^C
SspBI 572 TGTACA T^GTACA
StuI 1196 AGGCCT AGG^CCT
StyI 795 CCAAGG C^C[AT][AT]GG
TfiI 170 GAATC G^A[AT]TC
Tsp509I 152 AATT ^AATT
Tth111I 648 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1055 CCAACACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1319 CCATCAGCACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 943 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
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AlwI 944 GATCC GGATC(4/5)
943 GGATC
BalI 161 TGGCCA TGG^CCA
437 TGGCCA
BbrPI 531 CACGTG CAC^GTG
1059 CACGTG
BpmI 448 CTGGAG CTGGAG(16/14)
910 CTGGAG
BseRI 82 CTCCTC GAGGAG(10/8)
277 CTCCTC
BsmFI 142 GGGAC GGGAC(10/14)
237 GGGAC
FauI 636 GCGGG CCCGC(4/6)
800 GCGGG
MaeIII 262 GTCAC ^GT[ACGT]AC
764 GTGAC
MluNI 161 TGGCCA TGG^CCA
437 TGGCCA
MscI 161 TGGCCA TGG^CCA
437 TGGCCA
PmaCI 531 CACGTG CAC^GTG
1059 CACGTG
PmlI 531 CACGTG CAC^GTG
1059 CACGTG
PstI 478 CTGCAG CTGCA^G
607 CTGCAG
SacI 730 GAGCTC GAGCT^C
1241 GAGCTC
SfcI 478 CTGCAG C^T[GA][CT]AG
607 CTGCAG
Tsp45I 262 GTCAC ^GT[GC]AC
764 GTGAC
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# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
AvaI AvaII BanI BanII BbvI
BcnI BlpI BmyI Bpu1102I BsaAI
BsaJI BsaWI BsiHKAI BsiYI BslI
BsoBI Bsp1286I BsrI BssKI BssSI
BstNI BstUI BstXI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII Fnu4HI HaeII
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MaeII MboI MboII
MnlI MslI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SmaI
TseI TspRI XmaCI XmaI