Restriction map for
pF1KB6841
##################################
# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI Alw44I AlwI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspHI
AspI AvrII BalI BamHI BbrPI
BbsI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaWI BseAI BsgI
BsiHKAI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BsrI
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstYI BstZ17I CelII ClaI
CpoI DpnII DraI DraIII DrdI
Eco47III EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI HphI KpnI MaeI
MaeII MaeIII MamI MboI MfeI
MflI MluNI MroI MscI MseI
MunI NcoI NdeI NdeII NheI
NlaIII NotI NruI NsiI NspI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SalI SapI Sau3AI SexAI
SfaNI SfiI SfuI SgrAI SnaBI
SpeI SphI SspBI SspI SwaI
TfiI Tru9I Tsp45I Tsp509I TspRI
Tth111I Van91I XbaI XcmI XhoII
XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AfaI 177 GTAC GT^AC
AflIII 7 ACGCGT A^C[GA][CT]GT
AhdI 454 GACGGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 105 CCTCAGG CC^T[ACGT]AGG
AspEI 454 GACGGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI 321 CCTCAGC CCTCAGC(-5/-2)
BcgI 267 CGAGGGCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 469 GCCTCTTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 382 CTGGAG CTGGAG(16/14)
BsaI 287 GAGACC GGTCTC(1/5)
BseRI 502 GAGGAG GAGGAG(10/8)
BsiEI 413 CGGCCG CG[GA][CT]^CG
BsmI 442 GAATGC GAATGC(1/-1)
BsrBI 241 GAGCGG CCGCTC(-3/-3)
BstF5I 169 GGATG GGATG(2/0)
Bsu36I 105 CCTCAGG CC^T[ACGT]AGG
DsaI 91 CCGCGG C^C[GA][CT]GG
EaeI 413 CGGCCG [CT]^GGCC[GA]
EagI 413 CGGCCG C^GGCCG
Eam1105I 454 GACGGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 471 CTCTTC CTCTTC(1/4)
EclXI 413 CGGCCG C^GGCCG
Eco52I 413 CGGCCG C^GGCCG
Eco81I 105 CCTCAGG CC^T[ACGT]AGG
EcoT14I 464 CCAAGG C^C[AT][AT]GG
FokI 169 GGATG GGATG(9/13)
Ksp632I 471 CTCTTC CTCTTC(1/4)
KspI 91 CCGCGG CCGC^GG
MboII 472 TCTTC GAAGA(8/7)
MluI 7 ACGCGT A^CGCGT
MslI 164 CACGGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 415 GCCGGC GCC^GGC
NgoMIV 415 GCCGGC G^CCGGC
PaeR7I 403 CTCGAG C^TCGAG
PstI 370 CTGCAG CTGCA^G
RsaI 177 GTAC GT^AC
SacII 91 CCGCGG CCGC^GG
SbfI 369 CCTGCAGG CCTGCA^GG
ScaI 176 AGTACT AGT^ACT
SfcI 370 CTGCAG C^T[GA][CT]AG
SmaI 299 CCCGGG CCC^GGG
SmlI 403 CTCGAG C^T[CT][GA]AG
Sse8387I 369 CCTGCAGG CCTGCA^GG
StyI 464 CCAAGG C^C[AT][AT]GG
XhoI 403 CTCGAG C^TCGAG
XmaCI 299 CCCGGG C^CCGGG
XmaI 299 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AluI 305 AGCT AG^CT
377 AGCT
AlwNI 153 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
373 CAGGAGCTG
AvaII 74 GGACC G^G[AT]CC
99 GGACC
AviII 47 TGCGCA TGC^GCA
160 TGCGCA
BanI 418 GGCGCC G^G[CT][GA]CC
477 GGCGCC
BbeI 418 GGCGCC GGCGC^C
477 GGCGCC
BsmBI 4 GAGACG CGTCTC(1/5)
334 GAGACG
BspMI 293 ACCTGC ACCTGC(4/8)
368 ACCTGC
BsrFI 15 ACCGGC [GA]^CCGG[CT]
415 GCCGGC
BssHII 148 GCGCGC G^CGCGC
277 GCGCGC
Cfr10I 15 ACCGGC [GA]^CCGG[CT]
415 GCCGGC
Cfr13I 74 GGACC G^G[ACGT]CC
99 GGACC
DdeI 106 CTCAG C^T[ACGT]AG
322 CTCAG
DraII 73 GGGACCC [GA]G^G[ACGT]CC[CT]
98 GGGACCC
EcoO109I 73 GGGACCC [GA]G^G[ACGT]CC[CT]
98 GGGACCC
FspI 47 TGCGCA TGC^GCA
160 TGCGCA
HinfI 86 GACTC G^A[ACGT]TC
460 GAGTC
KasI 418 GGCGCC G^GCGCC
477 GGCGCC
NarI 418 GGCGCC GG^CGCC
477 GGCGCC
PleI 86 GACTC GAGTC(4/5)
460 GAGTC
PpuMI 73 GGGACCC [GA]G^G[AT]CC[CT]
98 GGGACCC
Sau96I 74 GGACC G^G[ACGT]CC
99 GGACC
SfoI 418 GGCGCC GGC^GCC
477 GGCGCC
StuI 392 AGGCCT AGG^CCT
467 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AccII AciI AcyI AvaI BanII
BbvI BcnI BmyI BsaHI BsaJI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BssKI BstAPI BstNI BstUI
Cac8I CfoI EcoRII FauI Fnu4HI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII ItaI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIV ScrFI TaqI TseI
TthHB8I