Restriction map for
pF1KE0673
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# NO CUT ENZYMES ( 170 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvI
BcgI BciVI BcnI BfrI BglI
BlnI BlpI BmyI BpmI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BstAPI BstBI BstEII BstPI BstXI
Cac8I CelII Cfr10I ClaI CpoI
DraI DrdI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FauI FseI
FspI HaeII HapII HincII HindII
HindIII HpaI HpaII KasI KpnI
Ksp632I KspI MamI MfeI MluI
MluNI MroI MscI MslI MspA1I
MspI MunI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI TseI TspRI Tth111I
Van91I XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 51 CGCG CG^CG
AcsI 516 GAATTT [GA]^AATT[CT]
AcyI 55 GACGCC G[GA]^CG[CT]C
AfaI 712 GTAC GT^AC
AluI 613 AGCT AG^CT
AlwI 665 GATCC GGATC(4/5)
AocI 67 CCTGAGG CC^T[ACGT]AGG
ApoI 516 GAATTT [GA]^AATT[CT]
BbsI 641 GAAGAC GAAGAC(2/6)
BbvCI 485 CCTCAGC CCTCAGC(-5/-2)
BclI 321 TGATCA T^GATCA
BglII 751 AGATCT A^GATCT
BpuAI 641 GAAGAC GAAGAC(2/6)
BsaHI 55 GACGCC G[GA]^CG[CT]C
BsaJI 472 CCAAGG C^C[ACGT][ACGT]GG
BsiYI 485 CCTCAGCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 485 CCTCAGCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 726 GCATTC GAATGC(1/-1)
BsrI 444 CCAGT ACTGG(1/-1)
BssSI 12 CACGAG CACGAG(-5/-1)
Bst1107I 690 GTATAC GTA^TAC
BstF5I 304 CATCC GGATG(2/0)
BstUI 51 CGCG CG^CG
BstZ17I 690 GTATAC GTA^TAC
Bsu36I 67 CCTGAGG CC^T[ACGT]AGG
CfoI 78 GCGC GCG^C
DraII 16 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 257 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 231 TGGCCG [CT]^GGCC[GA]
Eco81I 67 CCTGAGG CC^T[ACGT]AGG
EcoNI 485 CCTCAGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 16 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 472 CCAAGG C^C[AT][AT]GG
FbaI 321 TGATCA T^GATCA
Fnu4HI 143 GCCGC GC^[ACGT]GC
FokI 304 CATCC GGATG(9/13)
HgaI 55 GACGC GACGC(5/10)
HhaI 78 GCGC GCG^C
Hin1I 55 GACGCC G[GA]^CG[CT]C
HinP1I 78 GCGC G^CGC
ItaI 143 GCCGC GC^[ACGT]GC
MaeIII 741 GTCAC ^GT[ACGT]AC
MseI 410 TTAA T^TAA
MvnI 51 CGCG CG^CG
MwoI 190 GCTGAAGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 17 GGCCCC GG[ACGT]^[ACGT]CC
RsaI 712 GTAC GT^AC
SfcI 104 CTATAG C^T[GA][CT]AG
StuI 64 AGGCCT AGG^CCT
StyI 472 CCAAGG C^C[AT][AT]GG
Tru9I 410 TTAA T^TAA
Tsp45I 741 GTCAC ^GT[GC]AC
XbaI 526 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AvaII 542 GGTCC G^G[AT]CC
563 GGTCC
BfaI 527 CTAG C^TAG
611 CTAG
BsmAI 3 GTCTC GTCTC(1/5)
417 GTCTC
BssKI 62 CCAGG ^CC[ACGT]GG
277 CCAGG
BstNI 62 CCAGG CC^[AT]GG
277 CCAGG
BstYI 664 AGATCC [GA]^GATC[CT]
751 AGATCT
EcoRII 62 CCAGG ^CC[AT]GG
277 CCAGG
HinfI 415 GAGTC G^A[ACGT]TC
652 GACTC
HphI 262 GGTGA GGTGA(8/7)
595 TCACC
MaeI 527 CTAG C^TAG
611 CTAG
MflI 664 AGATCC [GA]^GATC[CT]
751 AGATCT
MvaI 62 CCAGG CC^[AT]GG
277 CCAGG
NlaIII 377 CATG CATG^
637 CATG
PleI 652 GACTC GAGTC(4/5)
415 GAGTC
ScrFI 62 CCAGG CC^[ACGT]GG
277 CCAGG
SfaNI 187 GATGC GCATC(5/9)
196 GATGC
XhoII 664 AGATCC [GA]^GATC[CT]
751 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AccI AciI Asp700I Cfr13I DdeI
DpnII HaeIII MaeII MboI MboII
MnlI NdeII Sau3AI Sau96I TaqI
Tsp509I TthHB8I XmnI