Restriction map for
pF1KE0685
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AvrII BalI BamHI BbeI BbrPI
BbsI BclI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaHI
BsaI BsaWI BseAI BsiWI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BssSI
Bst1107I BstAPI BstBI BstYI BstZ17I
CelII ClaI CpoI DraI DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoRI EcoRV
EcoT22I FbaI FseI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MflI MluI MluNI MroI MscI
MseI MunI NarI NcoI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I Tth111I Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AcsI 826 AAATTC [GA]^AATT[CT]
AfaI 837 GTAC GT^AC
AflIII 521 ACACGT A^C[GA][CT]GT
AlwNI 479 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 826 AAATTC [GA]^AATT[CT]
AviII 770 TGCGCA TGC^GCA
BanI 148 GGCACC G^G[CT][GA]CC
BbvCI 553 GCTGAGG CCTCAGC(-5/-2)
BcgI 773 GCAAGAAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 47 GTATCC GTATCC(6/5)
BfaI 760 CTAG C^TAG
BglI 56 GCCCACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 676 CTGGAG CTGGAG(16/14)
BsaAI 358 TACGTG [CT]AC^GT[GA]
BsgI 207 CTGCAC GTGCAG(16/14)
BsiEI 832 CGACCG CG[GA][CT]^CG
BsmAI 687 GAGAC GTCTC(1/5)
BsmBI 687 GAGACG CGTCTC(1/5)
BsmI 452 GCATTC GAATGC(1/-1)
BsrFI 629 GCCGGC [GA]^CCGG[CT]
BssHII 7 GCGCGC G^CGCGC
BstEII 615 GGTGACC G^GT[ACGT]ACC
BstPI 615 GGTGACC G^GT[ACGT]ACC
BstXI 53 CCAGCCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 629 GCCGGC [GA]^CCGG[CT]
DraIII 436 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 615 GGTGACC G^GT[ACGT]ACC
FspI 770 TGCGCA TGC^GCA
MaeI 760 CTAG C^TAG
MslI 522 CACGTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 629 GCCGGC GCC^GGC
NdeI 536 CATATG CA^TATG
NgoMIV 629 GCCGGC G^CCGGC
NlaIII 528 CATG CATG^
PvuII 766 CAGCTG CAG^CTG
RsaI 837 GTAC GT^AC
SfcI 260 CTGTAG C^T[GA][CT]AG
Tsp509I 827 AATT ^AATT
XcmI 369 CCAGAAGCCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwI 464 GGATC GGATC(4/5)
800 GGATC
AocI 496 CCTGAGG CC^T[ACGT]AGG
504 CCTGAGG
ApaI 408 GGGCCC GGGCC^C
815 GGGCCC
AspHI 128 GAGCAC G[AT]GC[AT]^C
640 GTGCTC
BsiHKAI 128 GAGCAC G[AT]GC[AT]^C
640 GTGCTC
BstF5I 252 CATCC GGATG(2/0)
325 CATCC
Bsu36I 496 CCTGAGG CC^T[ACGT]AGG
504 CCTGAGG
EarI 171 GAAGAG CTCTTC(1/4)
444 GAAGAG
Eco81I 496 CCTGAGG CC^T[ACGT]AGG
504 CCTGAGG
EcoT14I 395 CCTTGG C^C[AT][AT]GG
572 CCAAGG
FokI 252 CATCC GGATG(9/13)
325 CATCC
HphI 609 GGTGA GGTGA(8/7)
615 GGTGA
Ksp632I 171 GAAGAG CTCTTC(1/4)
444 GAAGAG
MaeII 359 ACGT A^CGT
523 ACGT
MaeIII 470 GTGAC ^GT[ACGT]AC
616 GTGAC
MspA1I 389 CAGCGG C[AC]G^C[GT]G
766 CAGCTG
PpuMI 32 GGGACCC [GA]G^G[AT]CC[CT]
587 GGGTCCC
SfaNI 735 GCATC GCATC(5/9)
756 GCATC
StyI 395 CCTTGG C^C[AT][AT]GG
572 CCAAGG
Tsp45I 470 GTGAC ^GT[GC]AC
616 GTGAC
TspRI 59 CACTG CA[GC]TG[ACGT][ACGT]^
468 CAGTG
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AluI AvaII BanII
BbvI BcnI BmyI BsaJI BseRI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII FauI Fnu4HI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MboI MboII MnlI MspI MvaI
MvnI MwoI NciI NdeII NlaIV
PleI Sau3AI Sau96I ScrFI TaqI
TseI TthHB8I