Restriction map for
pF1KB5796
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# NO CUT ENZYMES ( 96 enzymes) #
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AatII Acc65I AccI AclI AflII
AgeI AhdI Alw44I ApaLI AscI
AseI AsnI Asp718I AspEI AvaI
AviII AvrII BbvCI BcgI BciVI
BfaI BfrI BglII BlnI BsaBI
BsgI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspLU11I BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I CpoI DraI DrdI
Eam1105I EcoO65I EcoRI EcoT22I FseI
FspI HincII HindII HindIII HpaI
KpnI MaeI MamI MfeI MluI
MseI MunI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmeI PshBI Psp1406I
PvuI RsrII SalI SapI SbfI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AcsI 471 GAATTT [GA]^AATT[CT]
AflIII 1116 ACGTGT A^C[GA][CT]GT
AocI 1659 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1832 AGCGCT AGC^GCT
ApaI 1655 GGGCCC GGGCC^C
ApoI 471 GAATTT [GA]^AATT[CT]
Asp700I 1842 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1556 TGGCCA TGG^CCA
BbrPI 1115 CACGTG CAC^GTG
BclI 810 TGATCA T^GATCA
BlpI 1686 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1686 GCTCAGC GC^T[ACGT]AGC
BseRI 719 CTCCTC GAGGAG(10/8)
BsiEI 1218 CGGCCG CG[GA][CT]^CG
BspDI 2287 ATCGAT AT^CGAT
Bsu36I 1659 CCTGAGG CC^T[ACGT]AGG
CelII 1686 GCTCAGC GC^T[ACGT]AGC
ClaI 2287 ATCGAT AT^CGAT
DraIII 142 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1218 CGGCCG C^GGCCG
EclXI 1218 CGGCCG C^GGCCG
Eco47III 1832 AGCGCT AGC^GCT
Eco52I 1218 CGGCCG C^GGCCG
Eco81I 1659 CCTGAGG CC^T[ACGT]AGG
EcoNI 1293 CCTCATTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 810 TGATCA T^GATCA
HgaI 1703 GCGTC GACGC(5/10)
KspI 1793 CCGCGG CCGC^GG
MluNI 1556 TGGCCA TGG^CCA
MscI 1556 TGGCCA TGG^CCA
NaeI 1220 GCCGGC GCC^GGC
NgoMIV 1220 GCCGGC G^CCGGC
PflMI 1332 CCAATCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1115 CACGTG CAC^GTG
PmlI 1115 CACGTG CAC^GTG
PshAI 1698 GACCAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 2230 CAGCTG CAG^CTG
SacII 1793 CCGCGG CCGC^GG
ScaI 1973 AGTACT AGT^ACT
SexAI 1991 ACCTGGT A^CC[AT]GGT
StuI 2040 AGGCCT AGG^CCT
Van91I 1332 CCAATCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1842 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 921 CGCG CG^CG
1794 CGCG
AcyI 307 GGCGCC G[GA]^CG[CT]C
1927 GGCGCC
AlwNI 14 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1267 CAGTGGCTG
AspI 949 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1089 GACTCGGTC
BamHI 1628 GGATCC G^GATCC
2075 GGATCC
BbeI 307 GGCGCC GGCGC^C
1927 GGCGCC
BglI 275 GCCCCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1657 GCCCTGAGGGC
BsaHI 307 GGCGCC G[GA]^CG[CT]C
1927 GGCGCC
BsaI 733 GAGACC GGTCTC(1/5)
437 GGTCTC
BspHI 378 TCATGA T^CATGA
693 TCATGA
BsrBI 1110 CCGCTC CCGCTC(-3/-3)
2268 CCGCTC
BstUI 921 CGCG CG^CG
1794 CGCG
EcoRV 777 GATATC GAT^ATC
1153 GATATC
Hin1I 307 GGCGCC G[GA]^CG[CT]C
1927 GGCGCC
KasI 307 GGCGCC G^GCGCC
1927 GGCGCC
MvnI 921 CGCG CG^CG
1794 CGCG
NarI 307 GGCGCC GG^CGCC
1927 GGCGCC
PflFI 949 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1089 GACTCGGTC
PstI 2120 CTGCAG CTGCA^G
2227 CTGCAG
RcaI 378 TCATGA T^CATGA
693 TCATGA
SfoI 307 GGCGCC GGC^GCC
1927 GGCGCC
Tth111I 949 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
1089 GACTCGGTC
XcmI 480 CCACTATTCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1559 CCATGGCCGTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccIII AciI AfaI AluI AlwI
AspHI AvaII BanI BanII BbsI
BbvI BcnI BmyI BpmI BpuAI
BsaAI BsaJI BsaWI BseAI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspEI BspMI BsrFI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MroI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI PpuMI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII