Restriction map for
pF1KE0706
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvrII BamHI BbsI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BpuAI BsaBI BsaI
BsaWI BseAI BsgI BsiWI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I ClaI CpoI
DsaI Eam1105I Eco47III EcoO65I EcoRV
EcoT14I FbaI FseI HindIII HpaI
KpnI KspI MamI MflI MluI
MroI MvnI NcoI NdeI NheI
NotI NruI NspV PacI PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI PvuII RsaI RsrII SacII
SalI ScaI SexAI SfiI SfuI
SnaBI SpeI SphI SspBI SspI
StuI StyI SwaI TfiI Tth111I
XbaI XcmI XhoII
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# ONE CUT ENZYMES ( 55 enzymes) #
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AocI 841 CCTCAGG CC^T[ACGT]AGG
ApaI 1146 GGGCCC GGGCC^C
Asp700I 30 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 77 TGCGCA TGC^GCA
BalI 203 TGGCCA TGG^CCA
BbeI 13 GGCGCC GGCGC^C
BbrPI 672 CACGTG CAC^GTG
BfaI 223 CTAG C^TAG
BglI 669 GCCCACGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 672 CACGTG [CT]AC^GT[GA]
BsiEI 286 CGGCCG CG[GA][CT]^CG
BsmBI 980 CGTCTC CGTCTC(1/5)
BspMI 1048 GCAGGT ACCTGC(4/8)
Bsu36I 841 CCTCAGG CC^T[ACGT]AGG
DraI 172 TTTAAA TTT^AAA
DraIII 1189 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 502 GACCAGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 286 CGGCCG C^GGCCG
EclXI 286 CGGCCG C^GGCCG
Eco52I 286 CGGCCG C^GGCCG
Eco81I 841 CCTCAGG CC^T[ACGT]AGG
EcoNI 808 CCTCCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 356 GAATTC G^AATTC
EcoT22I 517 ATGCAT ATGCA^T
FspI 77 TGCGCA TGC^GCA
HincII 511 GTCAAC GT[CT]^[GA]AC
HindII 511 GTCAAC GT[CT]^[GA]AC
HinfI 72 GACTC G^A[ACGT]TC
KasI 13 GGCGCC G^GCGCC
MaeI 223 CTAG C^TAG
MfeI 366 CAATTG C^AATTG
MluNI 203 TGGCCA TGG^CCA
MscI 203 TGGCCA TGG^CCA
MslI 364 CACAATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1164 CAGCGG C[AC]G^C[GT]G
MunI 366 CAATTG C^AATTG
NarI 13 GGCGCC GG^CGCC
NsiI 517 ATGCAT ATGCA^T
NspI 515 ACATGC [GA]CATG^[CT]
PaeR7I 149 CTCGAG C^TCGAG
PleI 72 GACTC GAGTC(4/5)
PmaCI 672 CACGTG CAC^GTG
PmlI 672 CACGTG CAC^GTG
PstI 749 CTGCAG CTGCA^G
SacI 382 GAGCTC GAGCT^C
SapI 33 GCTCTTC GCTCTTC(1/4)
SbfI 748 CCTGCAGG CCTGCA^GG
SfaNI 267 GATGC GCATC(5/9)
SfcI 749 CTGCAG C^T[GA][CT]AG
SfoI 13 GGCGCC GGC^GCC
SgrAI 1155 CGCCGGCG C[GA]^CCGG[CT]G
Sse8387I 748 CCTGCAGG CCTGCA^GG
Tsp45I 415 GTCAC ^GT[GC]AC
XhoI 149 CTCGAG C^TCGAG
XmnI 30 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AcsI 169 AAATTT [GA]^AATT[CT]
356 GAATTC
AcyI 13 GGCGCC G[GA]^CG[CT]C
439 GACGCC
ApoI 169 AAATTT [GA]^AATT[CT]
356 GAATTC
AspHI 57 GAGCAC G[AT]GC[AT]^C
382 GAGCTC
BlpI 562 GCTGAGC GC^T[ACGT]AGC
1018 GCTCAGC
BpmI 54 CTGGAG CTGGAG(16/14)
812 CTGGAG
Bpu1102I 562 GCTGAGC GC^T[ACGT]AGC
1018 GCTCAGC
BsaHI 13 GGCGCC G[GA]^CG[CT]C
439 GACGCC
BseRI 929 CTCCTC GAGGAG(10/8)
758 GAGGAG
BsiHKAI 57 GAGCAC G[AT]GC[AT]^C
382 GAGCTC
BspHI 122 TCATGA T^CATGA
428 TCATGA
BsrFI 711 GCCGGC [GA]^CCGG[CT]
1156 GCCGGC
BstF5I 266 GGATG GGATG(2/0)
298 GGATG
CelII 562 GCTGAGC GC^T[ACGT]AGC
1018 GCTCAGC
Cfr10I 711 GCCGGC [GA]^CCGG[CT]
1156 GCCGGC
EarI 34 CTCTTC CTCTTC(1/4)
755 GAAGAG
FokI 266 GGATG GGATG(9/13)
298 GGATG
HaeII 13 GGCGCC [GA]GCGC^[CT]
588 AGCGCC
HgaI 439 GACGC GACGC(5/10)
1217 GACGC
Hin1I 13 GGCGCC G[GA]^CG[CT]C
439 GACGCC
HphI 373 GGTGA GGTGA(8/7)
785 TCACC
Ksp632I 34 CTCTTC CTCTTC(1/4)
755 GAAGAG
MaeII 673 ACGT A^CGT
1193 ACGT
MaeIII 415 GTCAC ^GT[ACGT]AC
1052 GTTAC
MboII 755 GAAGA GAAGA(8/7)
35 TCTTC
MseI 173 TTAA T^TAA
554 TTAA
NaeI 711 GCCGGC GCC^GGC
1156 GCCGGC
NgoMIV 711 GCCGGC G^CCGGC
1156 GCCGGC
PflMI 608 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
644 CCAATGGCTGG
PpuMI 309 AGGACCC [GA]G^G[AT]CC[CT]
1122 GGGTCCC
RcaI 122 TCATGA T^CATGA
428 TCATGA
SmaI 260 CCCGGG CCC^GGG
946 CCCGGG
SmlI 149 CTCGAG C^T[CT][GA]AG
1065 CTTGAG
Tru9I 173 TTAA T^TAA
554 TTAA
TspRI 497 CAGTG CA[GC]TG[ACGT][ACGT]^
613 CAGTG
Van91I 608 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
644 CCAATGGCTGG
XmaCI 260 CCCGGG C^CCGGG
946 CCCGGG
XmaI 260 CCCGGG C^CCGGG
946 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AluI AvaI AvaII BanI
BanII BbvI BcnI BmyI BsaJI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII FauI Fnu4HI
HaeIII HapII HhaI HinP1I HpaII
ItaI MboI MnlI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI TaqI TseI
Tsp509I TthHB8I