Restriction map for
pF1KE0719
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# NO CUT ENZYMES ( 146 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BalI BamHI
BbeI BbrPI BcgI BciVI BclI
BfaI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
BsmI BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstBI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRI
EcoT14I EcoT22I FbaI FseI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MluNI MroI
MscI MseI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI StuI StyI SwaI
Tru9I Tsp509I Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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Alw44I 208 GTGCAC G^TGCAC
AlwI 821 GGATC GGATC(4/5)
ApaLI 208 GTGCAC G^TGCAC
AviII 467 TGCGCA TGC^GCA
BbsI 670 GAAGAC GAAGAC(2/6)
BbvCI 246 CCTCAGC CCTCAGC(-5/-2)
BglII 152 AGATCT A^GATCT
BpuAI 670 GAAGAC GAAGAC(2/6)
BsaI 377 GAGACC GGTCTC(1/5)
BsgI 301 CTGCAC GTGCAG(16/14)
Bsp1407I 53 TGTACA T^GTACA
BsrGI 53 TGTACA T^GTACA
BstEII 687 GGTCACC G^GT[ACGT]ACC
BstPI 687 GGTCACC G^GT[ACGT]ACC
BstYI 152 AGATCT [GA]^GATC[CT]
EcoNI 246 CCTCAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 687 GGTCACC G^GT[ACGT]ACC
EcoRV 553 GATATC GAT^ATC
FauI 884 CCCGC CCCGC(4/6)
FspI 467 TGCGCA TGC^GCA
HapII 683 CCGG C^CGG
HpaII 683 CCGG C^CGG
MaeII 203 ACGT A^CGT
MflI 152 AGATCT [GA]^GATC[CT]
MslI 543 CACAGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 683 CCGG C^CGG
PflMI 480 CCATGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 292 GAGCTC GAGCT^C
SfaNI 572 GCATC GCATC(5/9)
SmaI 682 CCCGGG CCC^GGG
SmlI 163 CTCAAG C^T[CT][GA]AG
SspBI 53 TGTACA T^GTACA
TaqI 594 TCGA T^CGA
TthHB8I 594 TCGA T^CGA
Van91I 480 CCATGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 152 AGATCT [GA]^GATC[CT]
XmaCI 682 CCCGGG C^CCGGG
XmaI 682 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AccII 448 CGCG CG^CG
505 CGCG
AspHI 208 GTGCAC G[AT]GC[AT]^C
292 GAGCTC
AvaI 511 CCCGAG C^[CT]CG[GA]G
682 CCCGGG
BanII 292 GAGCTC G[GA]GC[CT]^C
868 GAGCCC
BcnI 682 CCCGG CC^[GC]GG
683 CCGGG
BpmI 558 CTGGAG CTGGAG(16/14)
763 CTGGAG
BsiHKAI 208 GTGCAC G[AT]GC[AT]^C
292 GAGCTC
BsmAI 73 GAGAC GTCTC(1/5)
377 GAGAC
BsoBI 511 CCCGAG C^[CT]CG[GA]G
682 CCCGGG
BspMI 395 ACCTGC ACCTGC(4/8)
782 ACCTGC
BstAPI 563 GCATTGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
792 GCAACTCCTGC
BstF5I 369 CATCC GGATG(2/0)
860 CATCC
BstUI 448 CGCG CG^CG
505 CGCG
BstXI 419 CCATCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
542 CCACAGCTGTGG
EarI 322 CTCTTC CTCTTC(1/4)
745 GAAGAG
FokI 369 CATCC GGATG(9/13)
860 CATCC
Ksp632I 322 CTCTTC CTCTTC(1/4)
745 GAAGAG
MvnI 448 CGCG CG^CG
505 CGCG
NciI 682 CCCGG CC^[GC]GG
683 CCGGG
TfiI 516 GATTC G^A[AT]TC
905 GATTC
TspRI 138 CACTG CA[GC]TG[ACGT][ACGT]^
494 CACTG
XcmI 731 CCAGGAAGGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
830 CCAAGACTGCCCTGG
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaII
BanI BbvI BmyI BsaJI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII EcoO109I EcoRII Fnu4HI
HaeIII HhaI HinP1I HinfI HphI
ItaI MaeIII MboI MboII MnlI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfcI
TseI Tsp45I