Restriction map for
pF1KE0723
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AlwI
AocI Aor51HI ApaI ApoI AscI
AseI AsnI Asp700I Asp718I AspI
AviII AvrII BalI BamHI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BsaAI
BsaBI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmI Bsp1407I BspEI
BspHI BspLU11I BspMI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CpoI
DraI DraIII DrdI DsaI EagI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HpaI KpnI
KspI MaeI MamI MfeI MflI
MluI MluNI MroI MscI MunI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI SexAI SfiI SfuI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Tsp509I Tth111I Van91I
XbaI XcmI XhoII XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 897 CGCG CG^CG
AcyI 685 GGCGCC G[GA]^CG[CT]C
Alw44I 157 GTGCAC G^TGCAC
ApaLI 157 GTGCAC G^TGCAC
AspHI 157 GTGCAC G[AT]GC[AT]^C
BanI 685 GGCGCC G^G[CT][GA]CC
BanII 847 GGGCTC G[GA]GC[CT]^C
BbeI 685 GGCGCC GGCGC^C
BbsI 67 GTCTTC GAAGAC(2/6)
BpmI 269 CTGGAG CTGGAG(16/14)
BpuAI 67 GTCTTC GAAGAC(2/6)
BsaHI 685 GGCGCC G[GA]^CG[CT]C
BsaI 115 GAGACC GGTCTC(1/5)
BsiHKAI 157 GTGCAC G[AT]GC[AT]^C
BsmBI 827 GAGACG CGTCTC(1/5)
BspDI 601 ATCGAT AT^CGAT
BsrBI 504 CCGCTC CCGCTC(-3/-3)
BsrDI 23 GCAATG GCAATG(2/0)
BsrI 412 CCAGT ACTGG(1/-1)
BstAPI 310 GCAGCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 900 GGATG GGATG(2/0)
BstUI 897 CGCG CG^CG
ClaI 601 ATCGAT AT^CGAT
DraII 907 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoNI 801 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 907 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 293 CCAAGG C^C[AT][AT]GG
FokI 900 GGATG GGATG(9/13)
HgaI 895 GACGC GACGC(5/10)
Hin1I 685 GGCGCC G[GA]^CG[CT]C
HindIII 10 AAGCTT A^AGCTT
HinfI 65 GAGTC G^A[ACGT]TC
KasI 685 GGCGCC G^GCGCC
MaeII 482 ACGT A^CGT
MseI 544 TTAA T^TAA
MslI 258 CATCAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 897 CGCG CG^CG
NarI 685 GGCGCC GG^CGCC
PaeR7I 62 CTCGAG C^TCGAG
PleI 65 GAGTC GAGTC(4/5)
PstI 559 CTGCAG CTGCA^G
ScaI 182 AGTACT AGT^ACT
SfcI 559 CTGCAG C^T[GA][CT]AG
SfoI 685 GGCGCC GGC^GCC
SgrAI 687 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 62 CTCGAG C^T[CT][GA]AG
StuI 798 AGGCCT AGG^CCT
StyI 293 CCAAGG C^C[AT][AT]GG
Tru9I 544 TTAA T^TAA
XhoI 62 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 183 GTAC GT^AC
484 GTAC
AhdI 43 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
440 GACCCCGGGTC
AlwNI 247 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
311 CAGCATCTG
AspEI 43 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
440 GACCCCGGGTC
BlpI 527 GCTCAGC GC^T[ACGT]AGC
612 GCTGAGC
Bpu1102I 527 GCTCAGC GC^T[ACGT]AGC
612 GCTGAGC
BsiYI 198 CCATGCCCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
801 CCTGCCCCAGG
BslI 198 CCATGCCCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
801 CCTGCCCCAGG
BsmAI 115 GAGAC GTCTC(1/5)
827 GAGAC
CelII 527 GCTCAGC GC^T[ACGT]AGC
612 GCTGAGC
CfoI 195 GCGC GCG^C
686 GCGC
Eam1105I 43 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
440 GACCCCGGGTC
EarI 379 CTCTTC CTCTTC(1/4)
868 GAAGAG
FauI 503 CCCGC CCCGC(4/6)
231 GCGGG
HaeII 194 AGCGCC [GA]GCGC^[CT]
685 GGCGCC
HhaI 195 GCGC GCG^C
686 GCGC
HinP1I 195 GCGC G^CGC
686 GCGC
Ksp632I 379 CTCTTC CTCTTC(1/4)
868 GAAGAG
NlaIII 199 CATG CATG^
264 CATG
PvuII 209 CAGCTG CAG^CTG
512 CAGCTG
RsaI 183 GTAC GT^AC
484 GTAC
SapI 868 GAAGAGC GCTCTTC(1/4)
378 GCTCTTC
SfaNI 604 GATGC GCATC(5/9)
313 GCATC
SmaI 203 CCCGGG CCC^GGG
443 CCCGGG
Tsp45I 448 GTCAC ^GT[GC]AC
517 GTCAC
XmaCI 203 CCCGGG C^CCGGG
443 CCCGGG
XmaI 203 CCCGGG C^CCGGG
443 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI AvaI AvaII BbvI
BcnI BmyI BsaJI BsmFI BsoBI
Bsp1286I BsrFI BssKI BstNI Cac8I
Cfr10I Cfr13I DdeI DpnII EaeI
EcoRII Fnu4HI HaeIII HapII HpaII
HphI ItaI MaeIII MboI MboII
MnlI MspA1I MspI MvaI MwoI
NaeI NciI NdeII NgoMIV NlaIV
Sau3AI Sau96I ScrFI TaqI TseI
TspRI TthHB8I