Restriction map for
pF1KE0778
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# NO CUT ENZYMES ( 153 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BalI BamHI BanII BbrPI
BbsI BbvCI BciVI BclI BcnI
BfrI BglI BlnI BpuAI BsaBI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI BspDI BspEI
BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT22I FauI FbaI FseI FspI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI MvnI
MwoI NaeI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StuI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 1344 GTCTAC GT^[AC][GT]AC
AciI 805 GCGG CCGC(-3/-1)
BanI 1513 GGCGCC G^G[CT][GA]CC
BbeI 1513 GGCGCC GGCGC^C
BcgI 923 GCATAGGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 711 AGATCT A^GATCT
BlpI 800 GCTTAGC GC^T[ACGT]AGC
BmyI 887 GTGCCC G[AGT]GC[ACT]^C
Bpu1102I 800 GCTTAGC GC^T[ACGT]AGC
BsaAI 950 TACGTG [CT]AC^GT[GA]
BsaI 1167 GAGACC GGTCTC(1/5)
BseRI 1330 GAGGAG GAGGAG(10/8)
Bsp1286I 887 GTGCCC G[AGT]GC[ACT]^C
BstF5I 588 GGATG GGATG(2/0)
CelII 800 GCTTAGC GC^T[ACGT]AGC
CfoI 1514 GCGC GCG^C
Cfr13I 870 GGCCC G^G[ACGT]CC
EcoRI 1041 GAATTC G^AATTC
EcoT14I 1479 CCAAGG C^C[AT][AT]GG
FokI 588 GGATG GGATG(9/13)
HaeII 1513 GGCGCC [GA]GCGC^[CT]
HapII 1511 CCGG C^CGG
HgaI 929 GCGTC GACGC(5/10)
HhaI 1514 GCGC GCG^C
HinP1I 1514 GCGC G^CGC
HincII 1140 GTTAAC GT[CT]^[GA]AC
HindII 1140 GTTAAC GT[CT]^[GA]AC
HindIII 988 AAGCTT A^AGCTT
HpaI 1140 GTTAAC GTT^AAC
HpaII 1511 CCGG C^CGG
KasI 1513 GGCGCC G^GCGCC
MaeII 951 ACGT A^CGT
MspA1I 1433 CAGCTG C[AC]G^C[GT]G
MspI 1511 CCGG C^CGG
NarI 1513 GGCGCC GG^CGCC
PstI 838 CTGCAG CTGCA^G
PvuII 1433 CAGCTG CAG^CTG
Sau96I 870 GGCCC G^G[ACGT]CC
SfoI 1513 GGCGCC GGC^GCC
SmlI 228 CTCAAG C^T[CT][GA]AG
SspI 360 AATATT AAT^ATT
StyI 1479 CCAAGG C^C[AT][AT]GG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcsI 605 AAATTC [GA]^AATT[CT]
1041 GAATTC
AcyI 928 GGCGTC G[GA]^CG[CT]C
1513 GGCGCC
AflIII 847 ACATGT A^C[GA][CT]GT
941 ACATGT
AlwI 1093 GATCC GGATC(4/5)
650 GGATC
ApoI 605 AAATTC [GA]^AATT[CT]
1041 GAATTC
BbvI 328 GCTGC GCAGC(8/12)
837 GCTGC
BfaI 1126 CTAG C^TAG
1418 CTAG
BsaHI 928 GGCGTC G[GA]^CG[CT]C
1513 GGCGCC
BsgI 149 GTGCAG GTGCAG(16/14)
1398 GTGCAG
BsmFI 853 GGGAC GGGAC(10/14)
1300 GTCCC
Bsp1407I 702 TGTACA T^GTACA
938 TGTACA
BspLU11I 847 ACATGT A^CATGT
941 ACATGT
BsrGI 702 TGTACA T^GTACA
938 TGTACA
BssKI 212 CCAGG ^CC[ACGT]GG
1289 CCTGG
BstNI 212 CCAGG CC^[AT]GG
1289 CCTGG
BstYI 711 AGATCT [GA]^GATC[CT]
1092 AGATCC
EcoRII 212 CCAGG ^CC[AT]GG
1289 CCTGG
Fnu4HI 328 GCTGC GC^[ACGT]GC
837 GCTGC
HaeIII 843 GGCC GG^CC
870 GGCC
Hin1I 928 GGCGTC G[GA]^CG[CT]C
1513 GGCGCC
ItaI 328 GCTGC GC^[ACGT]GC
837 GCTGC
MaeI 1126 CTAG C^TAG
1418 CTAG
MflI 711 AGATCT [GA]^GATC[CT]
1092 AGATCC
MslI 688 CACAATAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
780 CATAAAGGTG
MvaI 212 CCAGG CC^[AT]GG
1289 CCTGG
NspI 847 ACATGT [GA]CATG^[CT]
941 ACATGT
PleI 855 GACTC GAGTC(4/5)
1470 GACTC
ScrFI 212 CCAGG CC^[ACGT]GG
1289 CCTGG
SspBI 702 TGTACA T^GTACA
938 TGTACA
TfiI 154 GAATC G^A[AT]TC
1106 GAATC
TseI 328 GCTGC G^C[AT]GC
837 GCTGC
XhoII 711 AGATCT [GA]^GATC[CT]
1092 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AfaI AluI BpmI BsaJI BsiYI
BslI BsmAI BsrI Cac8I DdeI
DpnII EarI HinfI HphI Ksp632I
MaeIII MboI MboII MnlI MseI
NdeII NlaIII NlaIV RsaI Sau3AI
SfaNI SfcI Tru9I Tsp45I Tsp509I
TspRI